Occult Bacteremia in Young Children with Very High Fever Without a Source: A Multicenter Study

Gangoiti, Iker; Zubizarreta, Ane; Elgoibar, Borja; Mintegi, Santiago

doi:10.1097/inf.0000000000002891

Cited by 1 publication

(1 citation statement)

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“…Culturing pathogens has many benefits. It confirms a diagnosis, suggests potential infection sources, permits susceptibility testing, and in some cases, directing additional investigations or treatment durations [ 6 , 7 ]. There are also system benefits, including surveillance of antimicrobial resistance, central-line care, and assessing empiric antimicrobial guidance appropriateness [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Pediatric blood cultures—turning up the volume: a before and after intervention study

Whelan,

Mulrooney,

Moriarty

et al. 2024

Eur J Pediatr

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The major determinant of blood culture (BC) diagnostic performance is blood volume, and pediatric sample volumes are frequently low. We aimed to assess BC volumes in our institution, design an intervention to increase volumes, and assess its impact. All pediatric BCs submitted over a 7-month period to the microbiology laboratory in a university hospital (including emergency department, pediatric ward, and neonatal unit) were included. A pre-intervention period assessed current practice. A multi-faceted intervention (education, guideline introduction, active feedback strategies) was collaboratively designed by all stakeholders. Impact was assessed in a post-intervention period. The main outcome measures included the percentage of samples adequately filled using three measures of sample adequacy (1) manufacturer-recommended minimum validated volume—> 0.5 ml, (2) manufacturer-recommended optimal minimum volume—> 1.0 ml, (3) newly introduced age-specific recommendations. Three hundred ninety-eight pre-intervention and 388 post-intervention samples were included. Initial volumes were low but increased significantly post-intervention (median 0.77 ml vs. 1.52 ml), with multivariable regression analysis estimating volumes increased 89% post-intervention. There were significant increases in all measures of volume adequacy, including an increase in age-appropriate filling (20.4–53.1%), with less improvement in those aged > 3 years. Overall, 68.4% of pathogens were from adequately filled cultures, while 76% of contaminants were from inadequately filled cultures. A pathogen was detected in a higher proportion of adequately filled than inadequately filled cultures (9.4% vs. 2.2%, p < 0.001). Conclusion: Blood volume impacts BC sensitivity, with lower volumes yielding fewer pathogens and more contaminants. Focused intervention can significantly improve volumes to improve diagnostic performance. What is Known:• Blood volume is the major determinant of blood culture positivity, and yet pediatric blood culture volumes are frequently low, resulting in missed pathogens and increased contamination. What is New:• Adequately filled (for age) blood cultures have a pathogen detection rate three times higher than inadequately filled blood cultures.• This interventional study shows that collaboratively designed multi-modal interventions including focus on accurate volume measurement can lead to significant increases in blood volumes and improve blood culture diagnostic performance.

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Section: Introductionmentioning

confidence: 99%