Occlusion of Dentinal Tubules Using a Novel Dentifrice with Fluoro-Hydroxyapatite Nanoparticles Derived From A Biologic Source. (an in vitro study)

Abstract

Introduction: Dentin hypersensitivity is a widespread issue with high prevalence. Blocking the open dentinal tubules is one way to reduce dentin hypersensitivity. Aim: The current study aimed at evaluating the in vitro potential of a novel experimental dentifrice made of nano-fluoro hydroxyapatite (n-FHAP) produced from a biogenic source (cuttlefishbones) for occlusion of dentinal tubules. Material and Methods: 30 dentin discs (n = 30) were classified into 3 categories (n = 10) based on the dentifrices they utilized for brushing: 1 st category = artificial saliva (AS; control); 2 nd category = fluoride dentifrice (Sensodyne, UK); 3 rd category = experimental nano-FHAP dentifrice. Then, after brushing, discs were subjected to an acidic challenge with 6 percent wt citric acid (pH = 4.0) for 1 minute. By utilizing a SEM (scanning electron microscope) and EDX (energy-dispersive X-ray) spectroscopy before and after the citric acid challenge, the tubule occlusion was measured. Results: Group 1 (AS) SE micrographs revealed no tubule blockage. The tubules in Groups 2 and 3 were mostly blocked, according to SE micrographs. Data before and after the citric acid challenge data were statistically significant (p= 0.05) for all groups. Conclusion: In terms of tubule blockage, the findings of the new experimental dentifrice were equivalent to those of Sensodyne. Dentifrice containing FHAP nano-particles might be used to treat dentin sensitivity as an alternative.

Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care

“…Research investigations into the effectiveness of nanohydroxyapatite (nHAP) crystals within toothpaste formulations have revealed notable enhancements in the microhardness properties of human enamel following the application of toothpaste containing nHAPs [46]. Additionally, there is evidence of the proficient occlusion of dentinal tubules by nHAP particles, which adhere to the tooth surface and impede mineral dissolution processes [47]. Oral health as preventive and therapeutic agent, anti-inflammatory antioxidant, bactericidal effect, natural surfactants.…”