Occidiofungin is an important component responsible for the antifungal activity of <i>Burkholderia pyrrocinia</i>
 strain Lyc2

Wang, Xiaoqiang; Liu, Anxin; Guerrero, Adam; Liu, J.; Yu, Xianglong; Deng, Peng; Ma, Lin; Baird, Sonya M.; Smith, Linda L.; Li, X.D.; Lu, Shi‐En

doi:10.1111/jam.13036

Cited by 30 publications

(13 citation statements)

References 42 publications

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“…Burkholderia contaminans MS14 was shown previously to produce a potent antifungal named occidiofungin (17). In this study, zone-ofinhibition plate assays of strain MS14 grown on nutrient broth-yeast extract (NBY) demonstrated significant bactericidal activity against a broad array of plant bacterial pathogens ( Fig.…”

Section: Resultsmentioning

confidence: 99%

The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

Deng

Foxfire

et al. 2017

Appl Environ Microbiol

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Burkholderia contaminans MS14 was isolated from soil in Mississippi. When it is cultivated on nutrient broth-yeast extract agar, the colonies exhibit bactericidal activity against a wide range of plant-pathogenic bacteria. A bacteriostatic compound with siderophore activity was successfully purified and was determined by nuclear magnetic resonance spectroscopy to be ornibactin. Isolation of the bactericidal compound has not yet been achieved; therefore, the exact nature of the bactericidal compound is still unknown. During an attempt to isolate the bactericidal compound, an interesting relationship between the production of ornibactin and the bactericidal activity of MS14 was characterized. Transposon mutagenesis resulted in two strains that lost bactericidal activity, with insertional mutations in a nonribosomal peptide synthetase (NRPS) gene for ornibactin biosynthesis and a luxR family transcriptional regulatory gene. Coculture of these two mutant strains resulted in restoration of the bactericidal activity. Furthermore, the addition of ornibactin to the NRPS mutant restored the bactericidal phenotype. It has been demonstrated that, in MS14, ornibactin has an alternative function, aside from iron sequestration. Comparison of the ornibactin biosynthesis genes in Burkholderia species shows diversity among the regulatory elements, while the gene products for ornibactin synthesis are conserved. This is an interesting observation, given that ornibactin is thought to have the same defined function within Burkholderia species. Ornibactin is produced by most Burkholderia species, and its role in regulating the production of secondary metabolites should be investigated.IMPORTANCE Identification of the antibacterial product from strain MS14 is not the key feature of this study. We present a series of experiments that demonstrate that ornibactin is directly involved in the bactericidal phenotype of MS14. This observation provides evidence for an alternative function for ornibactin, aside from iron sequestration. Ornibactin should be further evaluated for its role in regulating the biosynthesis of secondary metabolites in other Burkholderia species.

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Section: Resultsmentioning

confidence: 99%

The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

Deng

Foxfire

et al. 2017

Appl Environ Microbiol

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“…The 16S rRNA gene is frequently used for phylogenetic classification of microorganism species (Woo et al ., 2008 ; Wang et al ., 2016 ). However, the resolving power of rDNA gene is often limited, particularly at the species level within Bacillus genus.…”

Section: Resultsmentioning

confidence: 99%

“…The antibacterial activity of the candidate strain was evaluated using a nutrient broth yeast (NBY) plate bioassay (Wang et al ., 2016 ). Escherichia coli FM3 , Pectobacterium carotovorum EC101, Burkholderia glumae 291, Pseudomonas syringae B301D, Xanthomonas campestris MSCT1 and R. solanacearum RS10 were used as indicator strains to test the antibacterial activity.…”

Section: Methodsmentioning

confidence: 99%

Genomic insights on fighting bacterial wilt by a novel Bacillus amyloliquefaciens strain Cas02

Chu

Ilyas

Peng

et al. 2021

Microbial Biotechnology

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Bacterial wilt, caused by the Ralstonia solanacearum, can infect several economically important crops. However, the management strategies available to control this disease are limited. Plant growth-promoting rhizobacteria (PGPR) have been considered promising biocontrol agents. In this study, Bacillus amyloliquefaciens strain Cas02 was isolated from the rhizosphere soil of healthy tobacco plants and evaluated for its effect on plant growth promotion and bacterial wilt suppression. Strain Cas02 exhibited several growth-promoting-related features including siderophore production, cellulase activity, protease activity, ammonia production and catalase activity. Moreover, strain Cas02 showed a significant inhibitory growth effect on R. solanacearum, and its active substances were separated and identified to be macrolactin A and macrolactin W by HPLC-DAD-ESI-MS/MS. Both greenhouse and field experiments demonstrated a good performance of Cas02 in plant growth promotion and bacterial wilt suppression. To explore the underlying genetic mechanisms, complete genome sequencing was performed and the gene clusters responsible for antibacterial metabolites expression were identified. Overall, these findings suggest that the strain Cas02 could be a potential biocontrol agent in bacterial wilt management and a source of antimicrobial compounds for further exploitation.

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“…Bakteri dari genus Burkholderia banyak dilaporkan memiliki peran dalam meningkatkan pertumbuhan tanaman, terutama karena aktivitas antifungi yang dihasilkannya. Selain enzim hidrolisis, senyawa lain seperti pirolnitrin (Sultan et al 2008), 1-metil-4-(1metiletenil)-sikloheksana (Elshafie et al 2012 burkholdin (Lin et al 2012), dan ocidiofungin (Wang et al 2016) yang dihasilkan oleh genus tersebut diketahui mampu menghambat pertumbuhan cendawan patogen tanaman, di antaranya Rhizoctonia solani dan F. oxysporum. Ocidiofungin yang diproduksi oleh B. contaminans juga dilaporkan sangat aktif terhadap cendawan patogen Pythium spinosum dan Pythium ultimum.…”

Section: Identifikasi Molekuler Bakteri Penghambat Fusariumunclassified

Penapisan Bakteri Penghambat Fusarium yang Diisolasi dari Cairan Kantung Semar (Nepenthes sp.)

Meliah

Hardiyanti

Haida

et al. 2020

J. Ilmu. Pertan. Indones.

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The genus Fusarium sp. is a pathogenic fungal for many cultivated plants. The bacteria isolated from monkey cup (Nepenthes sp.) fluid possess the ability to produce hydrolytic enzyme, such as chitinase which can be utilized to inhibit the growth of mycelia of pathogenic fungi. The aims of this study are to isolate bacteria from monkey cup liquid, to test their abilities to produce protease, chitinase, and cellulase, as well as their abilities to inhibit Fusarium. The bacteria were isolated using serial dilution method on Reasoner’s 2A agar medium. Enzymatic activities of bacterial isolates were determined by inoculating them on tested medium supplemented with casein protein, chitin, and cellulose, whereas their antifungal activities were assayed using a direct confrontation method between tested bacterial isolates and pathogenic fungal on Malt Extract Agar medium. Molecular identification of bacteria with antifungal activity was performed by analyzing the 16S rRNA gene sequences. Isolation process of bacteria from monkey cup fluid resulted in 99 bacterial isolates with the ability to produce either protease, chitinase, and/or cellulose enzymes. A total of 37 bacterial isolates were capable of producing at least two hydrolytic enzymes. Antifungal assay of those bacteria showed that as many as 25 isolates have the ability to inhibit the growth of Fusarium sp. Based on the analysis of 16S rRNA gene sequences revealed that those isolates were closely related to three Burkholderia species, namely B. arboris, B. contaminans, and B. rijonensis. Keywords: antifungal, Burkholderia, chitinase, cellulaseN, epenthes, protease

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Occidiofungin is an important component responsible for the antifungal activity of Burkholderia pyrrocinia strain Lyc2

Abstract: Understanding the mechanisms of antifungal activities of strain Lyc2 has contributed to discovery of new antibiotics and expand our knowledge of production of occidiofungin in the bacteria Burkholderia.

Cited by 30 publications

References 42 publications

The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

Genomic insights on fighting bacterial wilt by a novel Bacillus amyloliquefaciens strain Cas02

Penapisan Bakteri Penghambat Fusarium yang Diisolasi dari Cairan Kantung Semar (Nepenthes sp.)

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