Obtention par gynogenèse in vitro de plantes haploïdes chlorophyliennes chez le blé dur

Saldi, Najia; Chlyah, Averil; Chlyah, Hassan

doi:10.1016/s0764-4469(97)89622-9

Cited by 8 publications

(8 citation statements)

References 6 publications

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“…They are based on Sibi et al (2001) for M1 media (Induction medium: Ind M1; differentiation medium: Diff M1) and on modified Mdarhri-Alaoui et al (1998) for M2 media (Ind M2 based on (MS/2) Murashige and Skoog (1962); and Diff M2 based on MS without growth regulators). The development medium (Dev M) has the same composition as Diff M1, but lacks growth regulators.…”

Section: Culture Mediamentioning

confidence: 99%

“…A total of 12,000 unpollinated ovaries were used for this study. Cultures were sealed and kept in incubator under the dark condition (Sibi and Fakiri 1994;Mdarhri-Alaoui et al 1998) at 27°C for 5-6 weeks. Calli obtained were transferred to a differentiation medium (Diff M1 or Diff M2) ( Table 1) for 6 weeks at 25°C with a 16 h photoperiod at light intensity of 80-100 lE m -2 s -1 .…”

Section: Ovary Culture and Plant Regenerationmentioning

confidence: 99%

“…Gynogenesis has been showed to be an efficient way to ovoid this problem plants and was used to produce green double haploid plants in barley and wheat (San Noeum 1976;Mdarhri-Alaoui et al 1998;Sibi and Fakiri 1994;Sibi et al 2001). In sugar beet, it has been the most successful method for the production of doubled haploid plants (Gürel et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, in androgenesis, cold or mannitol pretreatment can influence the embryogenic development of the male gametophyte of wheat and induce the sporophytic development of embryos and callus (Hu and Kasha 1999). In gynogenesis, only cold treatment was used on durum wheat (Mdarhri-Alaoui et al 1998;Sibi et al 2001). In our present work, a 14-day cold pretreatment at 4°C or a 7-day cold mannitol pretreatment was applied to the spikes.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the successful use of unpollinated ovaries for such application requires optimized induction procedures to achieve a high regeneration frequency and refinements for each species. In durum wheat, two studies have been directed towards the cultivation of unpollinated ovaries: Mdarhri-Alaoui et al (1998) and Sibi et al (2001). The induction media component of these two studies are considerably different in the concentration of macro component, 2,4-D, vitamins, amino acids and carbohydrate source.…”

Section: Introductionmentioning

confidence: 99%

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Production of doubled haploids in durum wheat (Triticum durum Desf.) through culture of unpollinated ovaries

Slama-Ayed

Amara

2007

Plant Cell Tiss Organ Cult

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The objective of this work was to produce doubled haploid plants from durum wheat through gynogenesis using unpollinated ovary culture of three local Tunisian genotypes (Jenah Khotifa, Hmira, Azizi) and three improved cultivars (Karim, Khiar, Razzek). A total of 12,000 unpollinated ovaries were cultured in this study. Spikes were either pretreated at 4°C for 14 days or at 4°C in a mannitol solution (0.3 M) for 7 days. Induction was performed using two media. We showed that ovary development, callus and plantlet regeneration was influenced significantly by genotype and growth conditions. The highest regeneration frequency was obtained when the microspore population was in the late mononucleate to binucleate stage. Our results suggested that the cold pretreatment for 14 days was more efficient than the cold treatment in a mannitol solution. Furthermore, the addition of 2,4-D, vitamins and glutamine, and the use of maltose as sugar source in media improved the ovary culture. When the unpollinated ovaries were cultured under the conditions found to be optimal in the present study, a total of 84 plants were produced, all green and haploid.

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Section: Culture Mediamentioning

confidence: 99%

Section: Ovary Culture and Plant Regenerationmentioning

confidence: 99%