Obstructive sleep apnea syndrome promotes the progression of aortic dissection via a ROS- HIF-1α-MMPs associated pathway

Abstract

Aims: Obstructive sleep apnea syndrome (OSAS) has been increasingly recognized as an independent risk factor for aortic dissection (AD) and it is strongly associated with the extent of intermittent hypoxia and re-oxygenation (IH). This study aimed to clarify role of ROS- HIF-1α-MMPs pathway in the pathogenesis of AD and whether the HIF-1α inhibitor attenuates AD formation.Methods and results: 8-week-old male ApoE-/- mice were given β-aminopropionitrile at a concentration of 0.1 % for 3 weeks and infused via osmotic mini pumps with either saline or 2,500 ng/min/kg angiotensin II (Ang II) for 2 weeks. To mimic the OSAS, one group was exposed to IH, which consisted of alternating cycles of 20.9% O2/8% O2 FiO2 (30 episodes per hour) with 20 s at the nadir FiO2 during the 12-h light phase, 2 weeks before Ang II infusion. After Ang II infusion, we assessed remodeling in the aorta by echocardiography, histological and immunohistochemical analysis. IH treatment resulted in significant enlargement of the luminal area, destruction of the media, marked thickening of the adventitia, higher incidence of AD formation and lower survival rate in compared with the Ang II only group. Moreover, IH exposure markedly increased the aortic ROS production and subsequent HIF-1α expression, which in turn promoted the expressions of VEGF, MMP2 and MMP9 and finally leading to the progression of AD. Besides, in vitro study confirmed that IH induced HIF-1α expression plays an important role in the induction of MMPs and that is regulated by the PI3K/AKT/FRAP pathway. Intriguingly, a selective HIF-1α inhibitor KC7F2 could significantly ameliorate IH exposure induced aforementioned deleterious effects in vitro and in vivo.Conclusion: OSAS induced IH can promote the occurrence and progression of AD via a ROS- HIF-1α-MMPs associated pathway. The selective HIF-1α inhibitor KC7F2 could be a novel therapeutic agent for AD patient with OSAS.

Oxidative Stress and Inflammation Biomarker Expression in Obstructive Sleep Apnea Patients

“…However, despite obesity and hypertension are comorbidities associated with augmented chemoreflex patients, the CPAP treatment has not demonstrated significant results on the sympathetic system activity [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ,…”

“…The pathway consisting of reactive oxygen species induced by intermittent hypoxia (ROS) and the inducible transcription factor of hypoxia-1 (HIF-1) is responsible for deleterious cardiovascular outcomes, including aortic dissection development [ 21 ].…”

Oxidative Stress Markers among Obstructive Sleep Apnea Patients

“…It was postulated that aortic dissection may relate to ROS production induced by intermittent hypoxia and hypoxia-inducible factor-1 (HIF-1). Moreover, the progression of aortic dissection is also affected by this pathway, which promotes the expression of vascular endothelial growth factor (VEGF), as well as matrix metalloproteinases 2 and 9 in the aortic wall [19].…”

Extracellular Vesicles in Premature Aging and Diseases in Adulthood Due to Developmental Exposures

“…Altogether, this suggests that hypoxic conditions may result in a predisposition for osteoporosis and osteoarthritis. Developmental hypoxia comes in many forms: preeclampsia [ 184 ], placental insufficiency [ 185 ], gestational diabetes [ 186 ], childhood respiratory diseases [ 187 ], sleep apnea [ 188 ], etc.…”