Observing the devastating coffee berry borer (Hypothenemus hampei) inside the coffee berry using micro-computed tomography

Abstract

The coffee berry borer is the most devastating insect pest of coffee throughout the world. The insect spends most of its life cycle inside the coffee berry, which makes it quite difficult to observe its behaviour. Micro-computed tomography (micro-CT) was used to observe all developmental stages of the coffee berry borer inside coffee berries (Coffea canephora). An interesting oviposition pattern involving a sequential placement of eggs starting in the periphery of the seed and moving inwards was observed. Micro-CT should be useful in elucidating unknown life history aspects of other seed-feeding bark beetles as well as of bark and ambrosia beetles in general.

Revealing the respiratory system of the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae: Scolytinae) using micro-computed tomography

“…For a previous paper in which we first observed the coffee berry borer inside the coffee berry 40 , J.A.T. collected coffee berries ( Coffea canephora Pierre ex.…”

“…The coffee berry borer is the most devastating insect pest of coffee globally and poses a threat to coffee production due to its cryptic life habit inside the coffee berry, which makes it difficult to manage 39 . We have used modern micro-CT techniques to study this species and revealed various aspects of its biology inside coffee berries 40 , and the internal anatomy in adults 41 . In this paper, we present results from a detailed anatomical study of the tubular respiratory system of the coffee berry borer, including its spiracles.…”

3D visualisation of voids in grapevine flowers and berries using X‐ray micro computed tomography

“…3D X‐ray micro computed tomography (micro‐CT) imaging of plant components began in the late 1990s (Heeraman et al 1997) and has been expanding rapidly since. Micro‐CT has been applied to plants for visualising whole organ structure (Wang et al 2015), undisturbed root architecture (Tracy et al 2010), seed composition (Gargiulo et al 2019), inflorescence architecture (Li et al 2019), vascular tissue anatomy (Brodersen et al 2011, Wason et al 2017), xylem embolism (Cochard et al 2015), mineral and crystal deposition (Pierantoni et al 2017), cell volume (Herremans et al 2015), tissue porosity (Verboven et al 2015, Xiao et al 2018b, Signorelli et al 2020) and insect pest invasion (Alba‐Alejandre et al 2018). Generally, an X‐ray source is rotated around the specimen and the numerous shadow images on a rotating X‐ray detector at different angles can be reconstructed into a 3D data set made up of voxels (i.e.…”

Anatomical study of the female reproductive system and bacteriome of Diaphorina citri Kuwayama, (Insecta: Hemiptera, Liviidae) using micro-computed tomography

“…and behavioural/anatomical adaptations (e.g. [78][79][80][81][82]. Synchrotron X-ray tomography has been used to elucidate the general 3D configuration of the bacteriome of Orosius albicinctus (Hemiptera: Cicadellidae) 33 , and micro-CT has been used to locate and study the mycangia (cuticular cavities where symbiotic fungi proliferate 26,83 ) of ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) 65,73 .…”