Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms

Liu, Tsung‐Li; Upadhyayula, Srigokul; Milkie, Daniel E.; Singh, Ved; Wang, Kai; Swinburne, Ian A.; Mosaliganti, Kishore; Collins, Zachary; Hiscock, Tom W.; Shea, Jamien; Kohrman, Abraham; Medwig, Taylor N; Dambournet, Daphné; Forster, Ryan; Cunniff, Brian; Ruan, Yuan; Yashiro, Hanako; Scholpp, Steffen; Meyerowitz, Elliot M.; Hockemeyer, Dirk; Drubin, David G.; Martin, Benjamin L.; Matus, David Q.; Koyama, Minoru; Megason, Sean G.; Kirchhausen, Tom; Betzig, Eric

doi:10.1101/243352

Cited by 27 publications

(26 citation statements)

References 37 publications

(39 reference statements)

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“…The encouragement of GBM cells to migrate was part of certain biomaterial‐based paradigms to reduce brain tumor volume in vivo. [ 6 ] To enhance spatio‐temporal resolution of the cellular processes, a combined approach using automation of the matrix dispensation in the radial device together with real‐time measurements to study the migratory capability of the cells [ 30 ] and a quantitative approach to investigate changes in gene expression profiles are needed to better understand tumor cell dynamics. Besides dissecting cell–matrix interaction, the radial device is further suitable to investigate the migratory behavior of cells upon co‐culturing with other cell types present in the in vivo situation.…”

Section: Figurementioning

confidence: 99%

Melt Electrowritten In Vitro Radial Device to Study Cell Growth and Migration

et al. 2020

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of these scenarios. [1] Migration is influenced by chemotactic, topographical, and mechanotransductive cues from the extracellular matrix. [2] Moreover, cell migration in the context of cancer metastasis is a complex and important factor for understanding tumor progression. [3] The most aggressive form of a primary brain tumor is glioblastoma multiforme (GBM) which are highly invasive heterogenous tumors with a very low survival rate. [4] Surgical resection and chemo-or radiotherapy is commonly used for patient treatment, however, tumor recurrence is very frequent. Importantly, GBM cells invade and migrate along white matter tracts and brain blood vessels which promote tumor dissemination. [5] Hence, it is critical to understand the basic process of tumor migration and progression in order to develop new therapeutic drugs and treatment regimens. While therapeutic approaches that minimize GBM migration are logical, another approach focuses on guiding these cells away from the tumor into biomaterial reservoirs with the goal to reduce tumor size. [6] This diversional approach is based on the placement of a tube filled with a matrix and oriented substrate that provides topographical guidance cues at the tumor site. This results in the attraction and guidance of migration of GBM cells into the tube, effectively reducing overall tumor size. Therefore, in line with this study and the fact that GBM cells have an affinity for white brain matter and blood vessels, it is important to develop new 3D in vitro cell culture models to determine the optimal matrix composition that drives GBM migration. Novel research methods and tools provide an opportunity to study cell migration during cancer metastasis [7] where loss of cell adhesion from the primary tumor along with increased cell motility and invasion occurs. There is evidence from 3D microfluidic devices and microchips that matrix stiffness influences the migratory and invasive capabilities of tumor cells through the structure characteristics. [8] There have been significant advances to understand the process of cell migration using in vitro models, which are cost effective and easier to use compared to in vivo studies. With existing in vitro assays, cell migration conditions are welldefined with many based on the traditional 2D cell culture methods. [9] While simple to use, they are challenged to recapitulate the 3D in vivo microenvironment.

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Section: Figurementioning

confidence: 99%

Melt Electrowritten In Vitro Radial Device to Study Cell Growth and Migration

et al. 2020

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“…Although high-resolution optical microscopy [13][14][15] overcomes the drawbacks of diffraction-limited microscopy, it relies on the selectivity of labels or nanoparticles and does not resolve all ultrastructural features of the biological materials. So X-ray microscopy (SXM), exploiting recent advances in X-ray optics and synchrotron sources has led to unprecedented possibilities to investigate the 3D volumes of whole hydrated cells.…”

Section: Introductionmentioning

confidence: 99%

X-ray tomography shows the varying three-dimensional morphology of gold nanoaggregates in the cellular ultrastructure

et al. 2019

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“…Earlier this year research published in Science detailed how a team of scientists, led by Betzig, has created a microscope fast enough to track and record cells in living systems in 3D [3]. The research has accounted for many of the issues associated with super-resolution microscopy.…”

Section: Cells: the Stars Of Their Own 3d Moviesmentioning

confidence: 99%