Observing a Fictitious Stressful Event: Haematological Changes, Including Circulating Leukocyte Activation

Mian, Rubina; Shelton-Rayner, Graham K.; Harkin, Brendan; Williams, Paul A.

doi:10.1080/1025389031000101349

Cited by 20 publications

(12 citation statements)

References 26 publications

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“…Stressful stimuli also induce activation of neutrophils (Ellard et al 2001, Mian et al 2003, Keresztes 2013. The result is a rapid release of granule proteins but also de novo synthesis of cytokines by neutrophils themselves leading to amplification of the inflammatory response.…”

Section: Glucocorticoid Effects On Neutrophil Activationmentioning

confidence: 99%

Stress-induced release of matrix metalloproteinase-9 in patients with coronary artery disease: The possible influence of cortisol

Lundberg

Jonsson

Stenmark

et al. 2016

Psychoneuroendocrinology

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Stress-induced release of matrix metalloproteinase-9 in patients with coronary artery disease: The possible influence of cortisol, Psychoneuroendocrinology, 2016. 73() AbstractStress and inflammation are both important risk factors for coronary artery disease (CAD). However, the susceptibility to stress-induced inflammation and its determinants have been little explored in patients with CAD. Here, our aim was to study the stress-induced inflammatory response, more precisely the early release of matrix metalloproteinase (MMP)-9, and its association with cortisol response in patients with CAD. Sixty-four patients underwent a standardized laboratory stress test.The stress-induced release of MMP-9 was closely associated with the release of other neutrophilassociated proteins, MMP-8 and myeloperoxidase (MPO). It also showed a large variation among patients, as did cortisol. Twenty minutes after stress, a negative association between changes in MMP-9 and cortisol was seen (p<0.01). In vitro, dexamethasone reduced the IL-8-mediated release of MMP-9 from neutrophils, indicating that glucocorticoids may exert rapid effects on neutrophil activation. Further characterization of patients revealed that stress-induced release of MMP-9 was related to leukocyte telomere shortening and increased ultrasound-assessed plaque occurrence in the carotid arteries, but not to other characteristics such as age, gender or psychological background factors. The susceptibility to stress-induced release of MMP-9 may thus have impact on disease phenotype. Stress tests can be useful to identify CAD patients in need of novel prevention and treatment strategies.

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Section: Glucocorticoid Effects On Neutrophil Activationmentioning

confidence: 99%

Stress-induced release of matrix metalloproteinase-9 in patients with coronary artery disease: The possible influence of cortisol

Lundberg

Jonsson

Stenmark

et al. 2016

Psychoneuroendocrinology

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“…Stress has been shown to influence the number, distribution and activation state of neutrophils in the blood in a rapid and reversible manner (Dhabhar et al, 1995;Goebel and Mills, 2000;McLaren et al, 2003;Mian et al, 2003;Shelton-Rayner et al, 2011. When compared to the stressor paradigm utilised during this research, all previous examples utilised stressors that were of far greater duration and/or intensity.…”

Section: Introductionmentioning

confidence: 99%

Leukocyte responsiveness, a quantitative assay for subjective mental workload

Shelton-Rayner

Mian²,

Chandler

et al. 2012

International Journal of Industrial Ergonomics

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Assessing psychological stress and mental workload within work-based scenarios relies heavily upon qualitative, subjective, self-assessment techniques, many of which were originally intended for identifying specific pathological disorders and have reduced sensitivity when evaluating everyday stressors. Quantitative measures involve monitoring changes in the cardiopulmonary system and stress hormone concentration. Although these (e.g. heart rate and blood pressure) provide a basic, reactive indication of the presence of a psychological stressor, many are subject to influence by other bio-mechanisms, or are unable to provide rapid results due to complex laboratory analysis. This study demonstrates how immune responsiveness, known to be influenced by psychological stress, can be used to assess changes in mental workload. Healthy male and female subjects (aged between 26 and 55 years) provided capillary blood samples before and after completing the same, basic, driver-related tasks followed by a simple manoeuvre in two unfamiliar motor vehicles. Using a chemiluminescent technique termed Leukocyte Coping Capacity (LCC), the ability of leukocytes to produce reactive oxygen species in vitro was assessed. Significant post-stressor changes in leukocyte activity were demonstrated between treatment groups. These findings add weight to the proposition that leukocyte activation is a useful quantitative measure of psychological stress and mental loading in humans. This study demonstrates the diagnostic ability of LCC for use during ergonomic evaluation, however the potential industrial applications for this technique are numerous and diverse. KEY WORDSChemiluminescence; Ergonomics; Leukocytes; Mental Workload; Psychological Stress; Reactive Oxygen Species. 3 ABBREVIATIONSHmax-RLU adj , maximum control adjusted leukocyte activity; LCC, leukocyte coping capacity; RLU adj , control adjusted relative light units; T-max, time taken (minutes) to reach maximum control adjusted leukocyte activity; T=5, 10, and 15minutes, control adjusted leukocyte activity at 5, 10, and 15 minutes, respectively, into the 45 minute chemiluminescence sampling protocol. INTRODUCTIONEvaluation of psychological stress -a threat which would not require a physiological response which elicits physiological consequences (Segerstrom and Miller, 2004) relies heavily upon qualitative psychometric measures. Examples include: Present State Examination (Baker et al., 2003), the Brief Symptom Inventory (Brosig et al., 2007;Cuculi et al., 2006;Fellinger et al., 2007;Mansbach et al., 2005), and the BussDurkee Hostility Inventory (Bag et al., 2005). All were primarily designed to identify specific pathologic disorders, and validated using dysfunctional clinical populations with abnormal statistical distributions. Consequently these proved to have limited sensitivity when used to test below intended critical diagnostic thresholds (Lemyre and Tessier, 1988) -such as when investigating psychological and physiological transient laboratory stressors.As psychological...

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“…Psychological stress reduces the effectiveness of the immune system, thus leading to an increased risk of infection or disease [13,14]. Even shortterm psychological stressors such as academic examinations [15,16] can produce demonstrable physiological changes in the reactivity of specific classes of leukocyte, notably neutrophils [17].…”

Section: Introductionmentioning

confidence: 99%

Leukocyte reactivity as an objective means of quantifying mental loading during ergonomic evaluation

Shelton-Rayner

Macdonald

Chandler

et al. 2010

Cellular Immunology

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Psychological stress evokes rapid changes to the cardiovascular and neuroendocrine systems, responses that can become habituated following repeated exposure. This study, comprising of 2 phases, suggests that the immune system follows a similar trend. Phase 1: 15 healthy subjects (aged between 26 and 56 years) provided capillary blood samples before and after completing 3 basic tasks using, in turn, two automotive touch screen interfaces (Interface 1 -antecedent version, Interface 2 -improved version). Using a chemiluminescent technique termed Leukocyte Coping Capacity (LCC), the ability of leukocytes to produce reactive oxygen species in vitro was assessed. Significant differences in leukocyte activity were shown between treatment groups, where the greatest post-test decrease occurred after using Interface 1. Phase 2: a randomly selected sub-group (n=4) underwent weekly repeat testing using both interfaces. Significant differences in post-test leukocyte reactivity were exhibited between test weeks for each interface -the magnitude of response decreasing with successive exposure.

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Observing a Fictitious Stressful Event: Haematological Changes, Including Circulating Leukocyte Activation

Cited by 20 publications

References 26 publications

Stress-induced release of matrix metalloproteinase-9 in patients with coronary artery disease: The possible influence of cortisol

Stress-induced release of matrix metalloproteinase-9 in patients with coronary artery disease: The possible influence of cortisol

Leukocyte responsiveness, a quantitative assay for subjective mental workload

Leukocyte reactivity as an objective means of quantifying mental loading during ergonomic evaluation

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