Observations upon the structure of the nephron in the common eel

Grafflin, Allan L.

doi:10.1002/aja.1000610103

Cited by 20 publications

(7 citation statements)

References 22 publications

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“…In these animals, the arterial supply is feeble or absent, while the tubules are bathed almost entirely by portal venous blood. In the eel, the renal tubule is cytologically differentiated into regions consisting of proximal and distal tubules and a short collecting duct (Grafflin, 1937). Furthermore, there are anastomoses which divert part of the blood from the renal portal to the hepatic portal system (Mott, 1950 Grasse, 1958).…”

Section: Discussionsupporting

confidence: 39%

Renal Function in the European Eel (Anguilla Anguilla L.): Changes in Blood Pressure and Renal Function of the Freshwater Eel Transferred to Sea-Water

Jones¹,

Chan²,

Rankin³

1969

Journal of Endocrinology

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A method for the study of renal function and measurement of mean ventral and dorsal aortic blood pressure for the freshwater and seawater\x=req-\ adapted eel, and during transfer of the animal from fresh water to sea-water, is described.Freshwater eels have higher resting blood pressure, p-aminohippuric acid (PAH) and inulin clearance rates and urine flow than seawater eels. Urine from freshwater animals has low Na, K, Ca, Mg and Cl concentrations, while the clearance rate of inorganic phosphate exceeded that of inulin. Urine from seawater animals has high Na, Ca, Mg and Cl concentrations while that of inorganic phosphate was low. Clearance rates for Ca and Mg greatly exceeded those of inulin.During transfer from fresh water to sea-water there was an initial fluctuation in blood pressure, urine flow and PAH and inulin clearance rates which lasted about 2 hr. Thereafter these gradually declined to values observed for the seawater-adapted animal.The significance of PAH and inulin clearance rates in the study of renal function in the eel and in teleosts in general is discussed.

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Section: Discussionsupporting

confidence: 39%

Renal Function in the European Eel (Anguilla Anguilla L.): Changes in Blood Pressure and Renal Function of the Freshwater Eel Transferred to Sea-Water

Jones¹,

Chan²,

Rankin³

1969

Journal of Endocrinology

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“…Additionally, seasonal histological alteration has been reported in the American eel, Anguilla rostrata, caught in either salt or brackish water. Granular cells can be found in the proximal convoluted segment in kidney in winter A. rostrata, but not in summer animals (Grafflin 1937).…”

Section: In Our Light-microscopic Preparationsmentioning

confidence: 46%

Distinct Na+/K+/2Cl- cotransporter localization in kidneys and gills of two euryhaline species, rainbow trout and killifish

et al. 2008

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The kidney is an organ playing an important role in ion regulation in both freshwater (FW) and seawater (SW) fish. The mechanisms of ion regulation in the fish kidney are less well studied than that of their gills, especially at the level of transporter proteins. We have found striking differences in the pattern of Na(+)/K(+)/2Cl(-) cotransporter (NKCC) expression between species. In the killifish kidney, NKCC is apically localized in the distal and collecting tubules and basolaterally localized in the proximal tubules. However, in the SW killifish gill, NKCC is basolaterally co-localized with Na(+)/K(+)-ATPase, whereas in FW, NKCC immunoreactivity is primarily apical, although still colocalized within the same mitochondria-rich cell with basolateral Na(+)/K(+)-ATPase. Rainbow trout kidney has NKCC only in the apical membrane of the distal and collecting tubules in both environments, with no signal being detected in the proximal tubule. On the other hand, in the trout gill, NKCC is found basolaterally in both FW and SW environments. An important observation is that, in the gills of rainbow trout, the trailing edge of the filament possesses mostly Na(+)/K(+)-ATPase-positive but NKCC-negative mitochondria-rich cells, whereas in the region between and at the roots of the gill lamellae, most mitochondria-rich cells exhibit both Na(+)/K(+)-ATPase- and NKCC-positive immunoreactivity. These results suggest that the differential localization of transporters between the two species represents differences in function between these two euryhaline fishes with different life histories and strategies.

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“…Transition from one tubule segment to another was distinct with an immediate change from one cell type to another. Following the nomenclature established by Grafflin (1937), these segments were designated as: glomerulus, ciliated neck segment, proximal tubule 1 (P1), proximal tubule 2 (P2) and a distal tubule leading into a collecting tubule, which joined onto the branched collecting duct system. The ciliated neck segment was variable in length and comprised of a short columnar ciliated epithelium with predominantly oval-shaped nuclei that occupied a large proportion of the cytoplasm (Fig.…”

Section: Histologymentioning

confidence: 46%

“…A series of sections from each animal was kept for histological staining to determine the structure of the A. japonica nephron. Haematoxylin and eosin, periodic-acidSchiff and Masson's trichrome staining were used in conjunction with the description of Grafflin (1937) of the nephrons of A. rostrata and A. anguilla (formerly A. vulgaris) to identify the segments of the nephron that possessed immunopositive staining. A sample of slides used for immunohistochemistry was counter-stained with modified Masson's trichrome stain to confirm that staining surrounding the collecting ducts was indeed on the smooth muscle and not non-specific staining on the connective tissue surrounding these tubules.…”

Section: Immunohistochemistrymentioning

confidence: 49%

Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica

et al. 2005

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Natriuretic peptides are linked to osmoregulation, cardiovascular and volume regulation in fishes. The peptides bind to two guanylyl-cyclase-linked receptors, natriuretic peptide receptor-A (NPR-A) and NPR-B, to elicit their effects. Atrial natriuretic peptide (ANP) binds principally to NPR-A, whereas C-type natriuretic peptide (CNP) binds to NPR-B. The teleost kidney has an important role in the maintenance of fluid and electrolyte balance; therefore, the location of NPR-A and NPR-B in the kidney could provide insights into the functions of natriuretic peptides. This study used homologous, affinity purified, polyclonal antibodies to NPR-A and NPR-B to determine their location in the kidney of the Japanese eel, Anguilla japonica. Kidneys from freshwater and seawater acclimated animals were fixed overnight in 4% paraformaldehyde before being paraffin-embedded and immunostained. NPR-A immunoreactivity was found on the apical membrane of proximal tubule 1 and the vascular endothelium including the glomerular capillaries. In contrast, NPR-B immunoreactivity was located on the smooth muscle of blood vessels including the glomerular afferent and efferent arterioles, and on smooth muscle tissue surrounding the collecting ducts. No difference in the distribution of NPR-A and NPR-B was observed between freshwater and seawater kidneys. Immunoreactivity was not observed in any tissue in which the antibodies had been preabsorbed. In addition, there was no difference in NPR-A and NPR-B mRNA expression between freshwater-acclimated and seawater-acclimated eels. These results suggest that, although utilizing the same second messenger system, ANP and CNP act on different targets within the kidney and presumably elicit different effects.

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Observations upon the structure of the nephron in the common eel

Cited by 20 publications

References 22 publications

Renal Function in the European Eel (Anguilla Anguilla L.): Changes in Blood Pressure and Renal Function of the Freshwater Eel Transferred to Sea-Water

Renal Function in the European Eel (Anguilla Anguilla L.): Changes in Blood Pressure and Renal Function of the Freshwater Eel Transferred to Sea-Water

Distinct Na+/K+/2Cl- cotransporter localization in kidneys and gills of two euryhaline species, rainbow trout and killifish

Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica

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