Observations on the ultrastructure of large cells associated with putative neoplastic disorders of mussels, Mytilus edulis, from Yaquina Bay, Oregon

Mix, Michael C.; Hawkes, Joyce W.; Sparks, Albert K.

doi:10.1016/0022-2011(79)90052-1

Cited by 27 publications

(21 citation statements)

References 16 publications

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“…obs.). We therefore suggest that this group of viruses may prove to be common shellfish pathogens and that the neoplastic granulocytes reported by Lowe & Moore (1978) and Mix et al (1979) deserve further ultrastructural examination. Further work on the detailed properties and occurrence of this virus is under way.…”

Section: Discussionmentioning

confidence: 82%

“…Hine et al (1992) described culus is typical of the digestive gland described for a herpes-like virus associated with hatchery-reared several species of bivalve mollusc (Pal 1971(Pal , 1972 larval Pacific oysters Crassostrea gigas. An apparently Thompson et al 1974, Mix et al 1979, Henry 1984a, b, identical herpes-like virus also occurs in hatchery- Henry et al 1991). At the ultrastructural level, 3 types reared Tiostrea lutaria spat raised at elevated temper-atures (J B. Jones pers.…”

Section: Discussionmentioning

confidence: 99%

“…The strong positive staining with lead citrate is also characteristic of RNA (Hayat 1975). Mix et al (1979) studied hypertrophied Mytilus edulis granulocytes from Oregon, USA, which characterised a putative neoplastic disease. They reported that the large cells (15 pm diameter) present throughout the vesicular connective tissue were associated with pleomorphic, polymorphic nuclei, altered Golgi, mitochondrial crystalline inclusions and abundant free 'ribosomes'.…”

Section: Discussionmentioning

confidence: 99%

“…Lowe & Moore (1978) described similar large cells from M. edulis at Plymouth, U K . Mix et al (1979) concluded that though the cells possessed many properties characteristic of certain malignant vertebrate cells, the cause of the presumptive neoplastic disorder was unknown. A picorna-like virus has been found associated with granulocytomas in Danish M. edulis.…”

Section: Discussionmentioning

confidence: 99%

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Virus-like particles associated with marine mussel mortalities in New Zealand

Jones¹,

Scotti²,

Dearing³

et al. 1996

Dis. Aquat. Org.

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ABSTRACT. Green-lip mussel Perna canaliculus spat (15 to 30 m m length) in the outer Marlborough Sounds, New Zealand, suffered 50 to 100% mortal~t)! during January to April 1994 (summer/autumn) following thinning and reseeding of the mussel lines by farmers Adult mussel ~nortalities of 2 to 5 % continued from February to early h4ay 1994 Histolog~cal examination showed extensive haemocytosis and multifocal liquefact~on necrosls of i n t e r s t~t~a l cells, basal cells and digest~ve tubule ep~thelial cells. Sloughed pyknot~c 01-karyolyt~c digestive tubule epithelia1 cells formed charactenst~c rounded granular b o d~e s 10 to 15 pm diameter both In digestive tubules and free in lesions. No v~r a l inclusion bodies were observed Ultrastructural examination showed highly mod~fied rough endoplasn~ic reticulum assoc~ated with small, 25 to 45 nm diameter, electron-dense uncoated virus-like particles Identical cell damage and virus-like particles were subsequently found In moribund adult (75 to 110 mm length) P canallculus and stunted (25 to 47 mm length) subtidal 12-li'tilus galloprov~ncial~s from the same area.Following purification of extracts of the moribund spat b}! isopycnlc centl-ifugation in CsCI, large numbers of 25 nm diameter, unenveloped, virus pal-t~cles were scvn by electron mlcroscopy These part~cles had a density of 1.364 g cm-3 A broad band at a d e n s~t y c~xpected for enveloped particles (1 21 to 1 24 g cm ') was also observed but contained few v~rus-like particles Cell damage a n d mussel mortalit~es are thus likely due to a small unenveloped virus.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Virus-like particles associated with marine mussel mortalities in New Zealand

Jones¹,

Scotti²,

Dearing³

et al. 1996

Dis. Aquat. Org.

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“…Oprandy et al (1981) observed what they interpreted to be a type B retrovirus in a negatively stained sample of a sucrose gradient preparation from DN positive Mya arenaria. As reviewed in Elston et al (1992), several other electron microscopy studies have failed to detect the presence of viral particles associated with DN in M. arenaria (Farley 1976, Appeldoorn et al 1984, Mytilus edulis (Mix et al 1979, Moore 1993, or Cerastoderma edulis (Auffret & Poder 1986). The samples that were examined by negative staining techniques in this study were likewise negative for virus-like particles.…”

Section: Discussionmentioning

confidence: 99%

Soft shell clams Mya arenaria with disseminated neoplasia demonstrate reverse transcriptase activity

House

Kim²,

Reno³

1998

Dis. Aquat. Org.

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Disseminated neoplasia (DN), a proliferative cell disorder of the circulatory system of bivalves, was first reported in oysters in 1969. Since that time, the disease has been determined to be transmissible through water-borne exposure, but the etiological agent has not been unequivocally identified. In order to determine if a viral agent, possibly a retrovirus, could be the causative agent of DN, transmission experiments were performed, using both a cell-free filtrate and a sucrose gradientpurified preparation of a cell-free filtrate of DN positive materials. Additionally, a PCR-enhanced reverse transcriptase assay was used to determine if reverse transcriptase was present in tissues or hemolyrnph from DN positive soft shell clams Mya arenana. DN was transmitted to healthy clams by injection with whole DN cells, but not with cell-free filtrates prepared from either tissues from DN positive clams, or DN cells. The cell-free preparations from DN-positive tissues and hemolymph having high levels of DN cells in circulation exhibited positive reactions in the PCR-enhanced reverse transcriptase assay. Cell-free preparations of hemolymph from clams having low levels of DN (<0.1% of cells abnormal), hemocytes from normal soft shell clams, and normal soft shell clam tissues did not produce a positive reaction in the PCR enhanced reverse transcriptase assay.

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Cellular Defense Systems of the Mollusca

Fletcher

Cooper-Willis

1982

Phylogeny and Ontogeny

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Observations on the ultrastructure of large cells associated with putative neoplastic disorders of mussels, Mytilus edulis, from Yaquina Bay, Oregon

Cited by 27 publications

References 16 publications

Virus-like particles associated with marine mussel mortalities in New Zealand

Virus-like particles associated with marine mussel mortalities in New Zealand

Soft shell clams Mya arenaria with disseminated neoplasia demonstrate reverse transcriptase activity

Cellular Defense Systems of the Mollusca

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