Observations on the ultrastructure of lymphocystis virus in the Atlantic croaker, Micropogon undulatus (Linneaus)

Howse, Harold D.; Christmas, J. Y.

doi:10.1016/0042-6822(71)90166-8

Cited by 19 publications

(8 citation statements)

References 6 publications

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“…The presence of virus is confirmed by electron microscopy. Although virus has not yet been isolated, the disease has been experimentally transmitted by injection of affected erythrocytes or of filtrates of homogenized kidneys and spleen (Evelyn and Traxler, 1978; L. Nicholson, personal communication (Howse and Christmas, 1971;Walker, 1962;Walker and Sherburne, 1977;Wolf et at., 1966;Yamamoto et at., 1976). The capsid has icosahedral symmetry with lamellar construction and is separated from the nucleoid by a translucent zone (Fig.…”

Section: Piscine Erythrocytic Necrosis (Pen): Viral Erythrocytic Necrmentioning

confidence: 97%

Fish Viruses and Viral Infections

McAllister

1979

Comprehensive Virology

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Section: Piscine Erythrocytic Necrosis (Pen): Viral Erythrocytic Necrmentioning

confidence: 97%

Fish Viruses and Viral Infections

McAllister

1979

Comprehensive Virology

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“…Other ICDVs range from 130 nm to 300 nm; the largest and the one with the greatest reported size differences is lymphocystis virus. The sizes include 130-150 nm (Walker and Weissenberg, 1965),200 nm (Midlige and Malsberger, 1968), 240-260 nm (Howse and Christmas, 1971), and 300 nm (Zwillenberg and Wolf, 1968). This wide variation in size has several explanations: (1) the viruses measured were isolated from lymphocystis tumors of different species of fish and hence may represent different ICDVs associated with lymphocystis tumors; (2) dif- ferent methods of negative staining or of measuring thin sections were used, complicating the interpretation of results; and (3) lymphocystis virus may be inherently variable in size.…”

Section: Sizementioning

confidence: 98%

Icosahedral Cytoplasmic Deoxyriboviruses

Goorha

Granoff

1979

Comprehensive Virology

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“…Fragility of lymphocystis capsids was detected in comparison with frog virus 3 (FV3) and chilo iridescent virus (CIV) by Heppell & Berthiaume (1992). Thin capsids are also evident in images of previously reported lymphocystis virions from gourami (Paperna et al 1987) and other fish (Howse & Christmas 1971, Howse 1972, Lawler et al 1974; they are less evident in lymphocystis infecting Sparus aurata (Paperna et al 1982). Aubertin (1991) proposed placing visceral iridoviruses and lymphocystis in separate genera within the family Iridoviridae.…”

mentioning

confidence: 96%

Iridovirus infections in farm-reared tropical ornamental fish

Paperna¹,

Vilenkin²,

Matos³

2001

Dis. Aquat. Org.

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A systemic viral infection in both gourami Trichogaster spp. and swordtail Xiphophorus hellerii and an outbreak of lymphocystis in scalare Pterophyllum scalarae and gourami are reported to have occurred in fish reared in ornamental fish farms in Israel. The systemic infection developed in endothelial cells that became hypertrophic and their contents were modified. The presence of such cells in light-microscopically examined stained smears and sections provides an initial indication for this systemic viral infection. Infection in gourami caused hemorrhagic dropsy. Transmission electron microscopic (TEM) images of iridovirus-like particles recovered from gouramies showed them to be 138 to 201 nm from vertex to vertex (v-v); those from swordtails were 170 to 188 nm v-v. TEM images of lymphocystis virions from scalare were 312 to 342 nm v-v and from gourami 292 to 341 nm v-v. Lymphocystis cells from the gourami were joined by a solid hyaline plate, which was lacking in the infection in scalare where the intercellular spaces between the lymphocystis cells consisted of loose connective tissue.KEY WORDS: Trichogaster spp. · Xiphophorus spp. · Pterophyllum scalarae · Iridovirus-like particles · Systemic infection · Endothelium · Hypertrophy · Lymphocystis Resale or republication not permitted without written consent of the publisherDis Aquat Org 48: [17][18][19][20][21][22][23][24][25] 2001 findings together with a demonstration of viral particles by electron microscopy prove to be useful in the differential diagnosis of causes of mortality in the farmed fish. MATERIALS AND METHODSSmears and touch preparations were air-dried, fixed with absolute methanol and stained with Giemsa (in pH 7.4 buffer, for 1 h). For light microscopic histology, tissues were fixed in neutral-buffered formalin (exceptionally with aqueous Bouin's), and after dehydration in graded ethanols were embedded in glycol-methacrylate medium (Agar Scientific, Ltd, Stansted, UK). Sections, 2 to 3 µm, were cut with a glass knife on a Sorval JB4 microtome, and were stained with either Meyer's hemalum-eosin or, after a 20 min incubation in Bouin's solution and a water and 70% ethanol rinse, with 10% Giemsa in phosphate buffer, pH 7.4.For transmission electron microscopy (TEM), fragments from tissue with conspicuous cytopathological changes were fixed in 2.5% glutaraldehyde in cacodylate buffer (0.1 M, pH 7.4) for 24 h at 4°C, rinsed repeatedly in the same buffer, post-fixed in 1.0% osmium tetroxide in the same buffer for 1 h and, after rinsing in the buffer, dehydrated in graded ethyl alcohols and embedded in Agar 100 medium (Agar Scientific, Ltd). Thin sections, cut on a Reichert Ultracut Microtome with a diamond knife, were stained on grid with uranyl acetate and lead citrate, and examined with a Jeol 100CX TEM. Semi-thin sections cut with a glass knife were stained with Toluidine blue. RESULTS Viral hemorrhagic dropsy (VHD) in gouramiAffected fish, observed on several occasions in stocks of farmed gourami of the species Trichogaster trichopter...

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Observations on the ultrastructure of lymphocystis virus in the Atlantic croaker, Micropogon undulatus (Linneaus)

Cited by 19 publications

References 6 publications

Fish Viruses and Viral Infections

Fish Viruses and Viral Infections

Icosahedral Cytoplasmic Deoxyriboviruses

Iridovirus infections in farm-reared tropical ornamental fish

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