Observations on the social behaviour of cells in tissue culture

Abercrombie, M.; Heaysman, Joan E.M.

doi:10.1016/0014-4827(54)90176-7

Cited by 594 publications

(199 citation statements)

References 6 publications

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“…Hence, a change in direction of movement results from collision between two different cells, the net effect being that the cells migrate away from each other and the two cell populations fail to mix [6]. The degree of contact inhibition between two cell populations was originally studied using confronted explants [1].…”

Section: Introductionmentioning

confidence: 99%

The effects of modifying RhoA and Rac1 activities on heterotypic contact inhibition of locomotion

Anear

Parish

2012

FEBS Letters

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a b s t r a c tContact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or RhoA activities on heterotypic CIL between NIH3T3 and chicken heart fibroblasts. Both dominant active (L61) and dominant negative (N17) Rac1 expressed in NIH3T3 cells resulted in loss of heterotypic CIL. N17Rac1 expression caused RhoA activation. Increasing RhoA activity directly (V14RhoA) or indirectly (downregulation of N-cadherin or p120-catenin) also resulted in loss of CIL. High RhoA activity has been associated with tumour invasion and our results are consistent with loss of heterotypic CIL playing a role.

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Section: Introductionmentioning

confidence: 99%

The effects of modifying RhoA and Rac1 activities on heterotypic contact inhibition of locomotion

Anear

Parish

2012

FEBS Letters

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“…Studies of this type have shown that malignant transformation is often associated with changes in the surface properties of cells (1,2). Loss of postconfluence inhibition of cell division (3,4), loss of contact inhibition of cell movement (5), and changes in surface antigenicity (6,7) are thought to be related to such in vivo properties of tumors as loss of growth control, invasiveness, metastasis, and escape from immunological surveillance. In this regard, in vitro studies of cell adhesion have been of interest.…”

mentioning

confidence: 99%

Rates of aggregation, loss of anchorage dependence, and tumorigenicity of cultured cells.

Wright¹,

Ukena²,

Campbell³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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ABSTACr Ihe net rate of spontaneous aggregation of cells suspended with EDTA was measured for various cell types including spontaneous transformants and cells transformed with DNA and RNA viruses. The anchorage dependence as determined by growth in methyl cellulose and the tumorigenicity in vivo were also determined. All cells that had lost their anchorage dependency and were tumorigenic showed a high net rate of spontaneous adhesion. A31 was the only nontransformed cell line to have a high net rate of adhesion. Ihe net rate of spontaneous aggregation of cells is a quick and reliable index of tumorigenicity and offers a new approach to understanding the mechanisms of cell surface changes associated with transformation.One important approach to understanding the altered biology of malignant cells is comparison of the properties of cells grown in tissue culture with the characteristics of the same cells injected into experimental animals. Studies of this type have shown that malignant transformation is often associated with changes in the surface properties of cells (1, 2). Loss of postconfluence inhibition of cell division (3, 4), loss of contact inhibition of cell movement (5), and changes in surface antigenicity (6, 7) are thought to be related to such in vivo properties of tumors as loss of growth control, invasiveness, metastasis, and escape from immunological surveillance. In this regard, in vitro studies of cell adhesion have been of interest. It has been shown that transformation is associated with a decrease in cell adhesion to surfaces such as glass or plastic (8, 9). The effects of transformation on cell-to-cell adhesion are less clear, and different studies have produced conflicting results (10-13).Another surface property of cells that is affected by transformation is the ability to be agglutinated by plant lectins such as concanavalin A (Con A). Cultures used in these experiments were free of contamination by mycoplasma as judged by the uridine phosphorylase assay (24) and direct observation of cells by scanning electron microscopy.Aggregation Assay. The rate of aggregation of suspended cells was measured by the method described by Skehan and Friedman (25)

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“…In this regard, it is interesting to note that numerous close junctions, as well as occasional focal tight junctions, are observed between the mesoblast and ectoderm or endoderm during the period of migration of the mesoblast from the primitive streak. It is tempting to think that the adjoined areas guide the movement of the mesoblast and represent areas of contact inhibition (11,14). These tissues have not yet been studied for electrical coupling (mesoderm-ectoderm, mesoderm-endoderm).…”

Section: Discussionmentioning

confidence: 99%

Tight Junctions Between Cells in the Early Chick Embryo as Visualized With the Electron Microscope

Trelstad

Revel

Hay

1966

The Journal of Cell Biology

107

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Observations on the social behaviour of cells in tissue culture

Cited by 594 publications

References 6 publications

The effects of modifying RhoA and Rac1 activities on heterotypic contact inhibition of locomotion

The effects of modifying RhoA and Rac1 activities on heterotypic contact inhibition of locomotion

Rates of aggregation, loss of anchorage dependence, and tumorigenicity of cultured cells.

Tight Junctions Between Cells in the Early Chick Embryo as Visualized With the Electron Microscope

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