Observations on the pathogenesis of experimental Salmonella typhimurium infection in chickens

Barrow, Paul; Huggins, M. B.; Lovell, M. A.; Simpson, J. M.

doi:10.1016/s0034-5288(18)30685-4

Cited by 180 publications

(132 citation statements)

References 9 publications

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“…It is probable therefore that this type 13a strain of S. enteritidis multiplies poorly in these organs. This was one of the characteristics identified by Barrow et al (1987) that determine the virulence of S. typhimurium for young chicks. Despite the high frequency of isolation of phage type 4 strains from poultry and cases of food-poisoning the phage type 4, 6 and 8 strains tested in these experiments differed little in their virulence.…”

Section: Discussionmentioning

confidence: 92%

“…Rapid multiplication in the sterile intestine can result in shedding of very large numbers of Salmonella organisms within a few hours. The RIRs were more resistant to infection even though they are one of the more susceptible outbred breeds maintained at this laboratory (Barrow et al, 1987). These experimental infections in which chicks less than 24 h of age are inoculated orally with 10 s organisms thus represent a reasonable model with which to carry out future studies on virulence and disease control.…”

Section: Discussionmentioning

confidence: 94%

“…The results contrast with those of Hinton et al (1989) where no lesions were seen when broilers were infected with small numbers of organisms via the feed. The small inoculum administered over several days during which the chicks become increasingly resistant to Salmonella infection (Barrow et al, 1987) may have accounted for this. Where infection is transmitted vertically chicks may ingest organisms before, at or just after hatching.…”

Section: Discussionmentioning

confidence: 99%

“…(1938) as described by Barrow et al (1987). Viable counts were made on Brilliant Green agar containing 20 sodium nalidixate and 1 jUg/ml novobiocin.…”

Section: Bacterial Enumeration In Tissuesmentioning

confidence: 99%

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Experimental infection of chickens withSalmonella enteritidis

Barrow¹

1991

Avian Pathology

112

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SUMMARYFollowing oral inoculation of newly hatched commercial broilers, four phage types of Salmonella enteritidis showed considerable variation in their virulence. The percentage mortality varied from 96%, produced by a phage type 4 strain, to 20% (phage type 13a). When high mortality was observed, chickens that died showed a polyserositis with pericarditis similar to that observed in naturally occurring cases. Strains of phage types 4, 6 and 8 were more virulent than a phage type 13a strain when inoculated by the intramuscular route. Similar variations in virulence were observed in newly hatched Rhode Island Red chickens although the overall mortality observed was much reduced. Polyserositis was again observed.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

“…(1938) as described by Barrow et al (1987). Viable counts were made on Brilliant Green agar containing 20 sodium nalidixate and 1 jUg/ml novobiocin.…”

Section: Bacterial Enumeration In Tissuesmentioning

confidence: 99%

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Experimental infection of chickens withSalmonella enteritidis

Barrow¹

1991

Avian Pathology

112

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“…The association was greatest with strains of S. typhimurium. This serotype has been shown to be more virulent for chickens than other food-poisoning serotypes (Smith and Tucker, 1980a;Barrow et al, 1987a). The crop epithelium association may therefore be a reflection of the greater invasiveness of this serotype.…”

Section: Discussionmentioning

confidence: 96%

Intestinal colonisation in the chicken by food‐poisoningsalmonellaserotypes; Microbial characteristics associated with faecal excretion

Barrow¹,

Simpson²,

Lovell³

1988

Avian Pathology

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178

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SUMMARYFollowing oral inoculation of newly-hatched or three-week-old chickens, Salmonella organisms persisted longest in the caeca and they were also present for a shorter time in the crop. S. cholerae-suis, E. coli K12 and Saccharomyces cerevisiae did not colonise the alimentary tract when inoculated orally, but they persisted longer in the caeca than in other regions of the gut. This suggested that preferential localisation of Salmonella in the caeca may be the result of non-specific or host factors. This may include the slow rate of flow of contents through this organ which would allow greater microbial multiplication and persistence. Some Salmonella strains, particularly S. typhimurium, inoculated orally into three-week-old chickens were isolated in greater numbers from homogenates of the crop wall and, much less frequently, from the caecal wall, than from the luminal contents of these organs. None of the strains associated with the crop wall to the same extent as did lactobacilli.After oral inoculation of food-poisoning Salmonella serotypes the number of chickens excreting Salmonellae in their faeces gradually declined over a period of at least 4 weeks. In contrast, host adapted serotypes such as S. cholerae-suis and S. abortus-ovis were not excreted for longer than a few days. By testing mutants of S. typhimurium and S. infantis which were devoid of easily recognised characteristics it was concluded that neither flagellar (H) antigens, somatic (O) antigens, mannose-sensitive haemagglutinins nor the possession of the virulenceassociated 85 kilobase plasmid of S. typhimurium, were essential for colonisation (persistent faecal excretion). However, a mutant of S. infantis which was created by nitroso-guanidine treatment still possessed flagellar and somatic antigens and haemagglutinins and yet did not colonise the alimentary tract, indicating that an unidentified microbial factor was essential for colonisation.

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