Observations on the Morphology and Physiology of Marsilea Sperm

Rice, Harbert V.; Laetsch, W. M.

doi:10.1002/j.1537-2197.1967.tb10709.x

Cited by 26 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, several important observations were made particularly with respect to a band of tubular or fibrous structures close to the coiled nucleus, the manchette microtubules. These observations were confirmed recently in sperm of Marsilea and Zamia (Norstog 1967;Rice and Laetsch 1967), and a liverwort, Sphaerocarpos (Diers 1967). …”

Section: Introductionsupporting

confidence: 67%

“…Fritsch (1935, p. 458) says: "The liberated sperm appears prominently elongated, the accompanying cytoplasm being clearly marked at the anterior and posterior extremities ... ". Rice and Laetsch (1967) also found starch-containing plastids in Marsilea sperm. Some of the bodies seen at this region of the cell may be concerned with fertilization, but no data has yet been obtained on this point.…”

Section: Discussionmentioning

confidence: 82%

“…Manton and Clarke (1956) and Manton (1957Manton ( , 1959 showed a fibrous band in the coiled spermatozoids of Pteridium, Fucus (the "proboscis fibres"), and Sphagnum, which they concluded was significant in maintaining structural stability. Diers (1967) and Rice and Laetsch (1967) reported such microtubules in Sphaerocarpus and Marsilea, while Norstog (1967) associated these microtubules with "euglenoid" movement in Zamia sperm. Such tubules may also be involved in nuclear condensation with the concurrent elimination of some nuclear component inside a vesicle (Kessell 1966;Anderson, Weissman, and Ellis 1967).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrastructure and Differentiation in Chara (Fibrosa) IV. Spermatogenesis

Pickett‐Heaps¹

1968

Aust. Jnl. Of Bio. Sci.

103

View full text Add to dashboard Cite

SummarySpermatogenesis in Ohara is described at the ultrastructural level. A large nwnber of mitoses form spermatogenous threads, derived from the capitula inside the antheridiwn. Centrioles appear in the spermatogenous cells early as indistinct organelles that quickly become conspicuous. There is some evidence for the existence of a procentriole. Centrioles are subsequently associated in a normal fashion with the mitotic spindle. Intracellular differentiation commences after mitosis ceases. The nucleus moves to one side of the cell; a flat band of manchette microtubules is soon formed near it. The microtubules, which increase in nwnber and elongate considerably, are inserted at one end into (and possibly extruded from) a densely .staining, homogeneous inclusion, termed the manchette adjunct, which appears close to the centrioles. The centrioles, connected together by a spindle-shaped ciliary rootlet structure, move to the edge of the cell and start extruding flagella, which are covered in scales; another organelle, termed the vesicular adjunct and of unknown significance, appears near these centrioles. The manchette grows in length, and so asswnes a spiral course in the cell; plastids then line up along the microtubules next to the nucleus. While still interconnecting cells, the cytoplasm shrinks steadily. With further elongation of the manchette, the flagella apparatus moves away from the nucleus, and mitochondria also line up along the manchette tubules between them. Lipid (?) bodies move near the plastids, which steadily accumulate starch. Golgi bodies show marked structural changes during differentiation; they are initially associated with a profusion of various vesicles, and later lose their identity, as does the endoplasmic reticulwn which earlier interconnected cells through plasmadesmata. The nucleolus disappears, and later chromatin condensation gives the elongating nucleus an increasingly lamellate structure; finally these lamellae fuse to form a dense homogeneous nucleus. The cytoplasm continues to shrink, eliminating almost all cell organelles. The mature spermatozoid, tightly coiled in the cell, finally contains plastids and (lipoid) inclusions at one end, next to the dense, elongate nucleus, with linearly arranged mitochondria at the other end, and flagella inserted above the mitochondria. Manchette microtubules run the length of the organism as a flat band opposed to the nucleus and plastids, and finally as a tubular sheath partly enclosing the mitochondria. Four other tubules, possibly derived from a ciliary root structure, are also close to the mitochondria. The flagella are quite long by this stage. The observations are discussed in terms of the functions of cell organelles. In particular, it is suggested that centriolar movement in mitosis may be only one example of several morphogenic movements associated with microtubule organization; their function is in flagella formation, and not in synthesis of spindle, manchette, or other cytoplasmic microtubules.• Part III, AUBt.

show abstract

Section: Introductionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrastructure and Differentiation in Chara (Fibrosa) IV. Spermatogenesis

Pickett‐Heaps¹

1968

Aust. Jnl. Of Bio. Sci.

103

View full text Add to dashboard Cite

show abstract

“…Despite these and other excellent studies (Tralau 1969;Ferrarini et al 1986;Large andBraggins 1989, 1991;Stafford 1995), spore character variation within Marsileaceae has not been well documented, in part because important layers that can be viewed only with light microscopy, such as the gelatinous perine layer, were neglected. A gelatinous "spore envelope" has been reported in Marsilea (Shattuck 1910;Feller 1953;Machlis and Rawitscher-Kunkel 1967;Rice and Laetsch 1967;Pettitt 1971;Bilderback 1978;Pettitt 1979aPettitt , 1979b, Pilularia (Meunier 1888) and Regnellidium (Lindman 1904;Chrysler and Johnson 1939;Higinbotham 1941), but it has never been examined in detail or compared among genera. A second interesting but underexamined feature of marsileaceous spores is the modified perine above the aperture, which has been variously referred to either as an acrolamella, hilum, or papilla.…”

Section: Introductionmentioning

confidence: 99%

Structure and Function of Spores in the Aquatic Heterosporous Fern Family Marsileaceae

Schneider

Pryer

2002

International Journal of Plant Sciences

View full text Add to dashboard Cite

Spores of the aquatic heterosporous fern family Marsileaceae differ markedly from spores of Salviniaceae, the only other family of heterosporous ferns and sister group to Marsileaceae, and from spores of all homosporous ferns. The marsileaceous outer spore wall (perine) is modified above the aperture into a structure, the acrolamella, and the perine and acrolamella are further modified into a remarkable gelatinous layer that envelops the spore. Observations with light and scanning electron microscopy indicate that the three living marsileaceous fern genera (Marsilea, Pilularia, and Regnellidium) each have distinctive spores, particularly with regard to the perine and acrolamella. Several spore characters support a division of Marsilea into two groups. Spore character evolution is discussed in the context of developmental and possible functional aspects. The gelatinous perine layer acts as a flexible, floating organ that envelops the spores only for a short time and appears to be an adaptation of marsileaceous ferns to amphibious habitats. The gelatinous nature of the perine layer is likely the result of acidic polysaccharide components in the spore wall that have hydrogel (swelling and shrinking) properties. Megaspores floating at the water/air interface form a concave meniscus, at the center of which is the gelatinous acrolamella that encloses a "sperm lake." This meniscus creates a vortex-like effect that serves as a trap for free-swimming sperm cells, propelling them into the sperm lake.

show abstract

“…Dans le monde végétal, le groupe des Ptéridophytes filicinées hydroptéridées constitue un petit ensemble de plantes parmi les plus intéressantes car leur reproduction, zoïdogame, se réalise grâce à des spermato-(*)Manuscrit reçu le 21 janvier 1988; accepté le 11 février 1988. zoïdes multiflagellés selon un mode assez proche de celui des animaux. Dans ce groupe, le Marsilea vestita a fait l'objet d'un certain nombre de travaux aussi bien en France (Andrès, 1964 ;Tourte et coll., 1971 ;Kuligowski-Andrès, 1975a et b ;Tourte et Kuligowski-Andrès, 1980 ;Tourte et coll., 1980 ;Faivre et coll., 1982 ;Bordonneau et coll., 1984;Kermarrec et Tourte, 1984) qu'aux U.S.A. (Rice et Laetsch, 1967;Hepler, 1972 ;Myles et Bell, 1975 ;Bilderback, 1978 a et b ;Kurth, 1981 ;Myles et Hepler, 1982), et la connaissance des mécanismes cytologiques de la reconnaissance puis de la fusion gamétique ont pu faire, grâce à cette espèce, d'importants progrès. Elle présente, en effet, de très nombreux avantages, en particulier de pouvoir être cultivée en conditions axéniques et de réaliser en un temps très court -8 à 9 heures -la totalité de la prothallogenèse et de la gamétogenèse.…”

Section: Introductionunclassified

Deux hydroptéridées indigènesPilularia globuliferaL. etMarsilea quadrifoliaL. Un nouveau matériel pour l'étude de la reproduction sexuéein vitro

Bordonneau

Tourte

1988

Bulletin de la Société Botanique de France. Lettres Botaniques

View full text Add to dashboard Cite

Résumé.-Une étude comparative de la gamétogenèse de deux espèces de Ptéridophytes aquatiques indigènes : le Pilularia globulifera L. et le Marsilea quadrifolia L, espèces qui n'avaient fait jusqu'ici l'objet que de travaux anciens, a été réalisée. Il apparait que si le Marsilea quadrifolia présente une séquence gamétogène très proche de celle du Marsilea vestita -espèce de loin la plus étudiée -par contre, le Pilularia globulifera en diffère quelque peu avec, en particulier, la présence d'un sillon sur la mégaspore et l'existence de spermatozoïdes présentant uniquement deux tours de spires nucléaires et une forme circulaire, contre 8 à 10 tours et une forme conique chez le Marsilea.Ces différences et ces similitudes nous laissent entrevoir l'intérêt qu'apporte l'utilisation de ces deux espèces indigènes dans l'étude de phénomènes aussi fondamentaux que l'hybridation et l'évolution des chromatines parentales au cours des tous premiers stades de l'embryogenèse.Summary.-A comparative study of gametogenesis in two species of native aquatic Ferns Pilularia globulifera and Marsilea quadrifolia has been undertaken. These two Ferns have not been studied for a long time. lt appears that if Marsilea quadrifolia shows gametogenic sequences very similar to that of Marsilea vestita -which has been the most widely studied species so far -on the contrary, Pilularia globulifera exhibits differences, in particular : a groove on the megaspore and spermatozoa with only 2 turns of nuclear co il and a circular form, as opposed to 8 to 10 turns and a cone shape in the case of Marsilea. These differences and similarities show that it is of great interest to use these 2 native species for the study of such fundamental processes as hybridization and evolution of parental chromatins during the early stages of embryogenesis.

show abstract

Observations on the Morphology and Physiology of Marsilea Sperm

Cited by 26 publications

References 16 publications

Ultrastructure and Differentiation in Chara (Fibrosa) IV. Spermatogenesis

Ultrastructure and Differentiation in Chara (Fibrosa) IV. Spermatogenesis

Structure and Function of Spores in the Aquatic Heterosporous Fern Family Marsileaceae

Deux hydroptéridées indigènesPilularia globuliferaL. etMarsilea quadrifoliaL. Un nouveau matériel pour l'étude de la reproduction sexuéein vitro

Contact Info

Product

Resources

About