Observations on the Biological Roles of Sulphatases

Dodgson, K. S.; Rose, F. A.

doi:10.1002/9780470720554.ch10

Cited by 4 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high concentration of sulfated quercetin in F. pringlei indicates that it may serve as a storage of sulfate. The sulfate might be remobilized by enzymatic reaction catalyzed by sulfatase, which is present in bacteria as well as humans and animals (Dodgson and Rose, 1979). However, to date sulfatase has not been detected in plants.…”

Section: Discussionmentioning

confidence: 99%

A Novel Method for Identification and Quantification of Sulfated Flavonoids in Plants by Neutral Loss Scan Mass Spectrometry

et al. 2019

View full text Add to dashboard Cite

Sulfur is present in plants in a large range of essential primary metabolites, as well as in numerous natural products. Many of these secondary metabolites contain sulfur in the oxidized form of organic sulfate. However, except of glucosinolates, very little is known about other classes of such sulfated metabolites, mainly because of lack of specific and quantitative analytical methods. We developed an LC-MS method to analyze sulfated flavonoids, a group of sulfated secondary metabolites prominent, e.g., in plants of the genus Flaveria . The method uses a linear gradient of methanol/formic acid in water on a Restek Raptor C 18 Core-Shell column for separation of the compounds. The sulfated flavonoids are detected by mass spectrometry (MS) in a negative mode, using a neutral loss of 80 Da after a collision induced dissociation. With this method we were also able to quantify the sulfated flavonoids. We could detect all (mono)sulfated flavonoids described before in Flaveria plus a number of new ones, such as isorhamnetin-sulfate-glycoside. In addition, we showed that sulfated flavonoids represent a substantial sulfur pool in Flaveria , larger than the thiols glutathione and cysteine. The individual species possess different sulfated flavonoids, but there is no correlation between the qualitative pattern and type of photosynthesis. Similar to other sulfur-containing secondary compounds, the concentration of sulfated flavonoids in leaves is reduced by sulfur starvation. The new LC-MS method will enable qualitative and quantitative detection of these secondary metabolites in plants as a pre-requisite to addressing their functions.

show abstract

Section: Discussionmentioning

confidence: 99%

A Novel Method for Identification and Quantification of Sulfated Flavonoids in Plants by Neutral Loss Scan Mass Spectrometry

et al. 2019

View full text Add to dashboard Cite

show abstract

Sulfates

Huxtable¹

1986

Biochemistry of Sulfur

View full text Add to dashboard Cite

Sulfur regulation of heparinase and sulfatases in Flavobacterium heparinum

Cerbelaud¹,

Conway²,

Galliher³

et al. 1986

Appl Environ Microbiol

View full text Add to dashboard Cite

Sulfur regulation of heparinase synthesis and sulfatase synthesis was studied in Flavobacterium heparinum. Heparinase synthesis was strongly repressed by sulfate and L-cysteine, while the activity of this enzyme showed little or no inhibition by these compounds. Heparinase was synthesized in the absence of heparin when L-methionine was used as the sole sulfur source. The sulfatases produced by F. heparinum, which include the sulfatases involved in heparin catabolism, were also studied. At least some of the sulfatase activity was regulated by sulfur compounds in a manner similar to heparinase regulation. L-Cysteic acid and taurine were not suitable sulfur sources to support the growth of F. heparinum.

show abstract

Observations on the Biological Roles of Sulphatases

Cited by 4 publications

References 27 publications

A Novel Method for Identification and Quantification of Sulfated Flavonoids in Plants by Neutral Loss Scan Mass Spectrometry

A Novel Method for Identification and Quantification of Sulfated Flavonoids in Plants by Neutral Loss Scan Mass Spectrometry

Sulfates

Sulfur regulation of heparinase and sulfatases in Flavobacterium heparinum

Contact Info

Product

Resources

About