Observations on Spermatogenesis in Rats

Cutuly, Eugene; Cutuly, Elizabeth C.

doi:10.1210/endo-26-3-503

Cited by 24 publications

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“…In hypophysectomized animals spermatogenic activity may be maintained by injections of androgen [Cutuly & Cutuly, 1940;Chu, 1940; and earlier reports by others] or when the testes have atrophied spermatogenesis may be partially restored in the same manner [Selye & Friedman, 1941]. The present report describes similar results in male pigeons and ovarian stimulation following androgen injection in female pigeons.…”

supporting

confidence: 85%

Gonad Stimulation by Androgens in Hypophysectomized Pigeons

Chu¹,

You²

1944

Journal of Endocrinology

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There have been many contradictory reports of the effects of androgens on the testes. In the rat the most frequently noted effect has been an adverse one (see review by Moore [1939]), and in the pigeon, too, atrophy of the testes has been produced by testosterone injections [Chu, 1940]. This adverse effect is presumably due to an inhibition of gonadotrophin production or secretion.Pigeons obtained from a local dealer were kept in cages (ten per cage) and given wheat and corn twice daily with occasional vegetables. The birds were sexed by laparotomy and hypophysectomy was performed trans-buccally [Chu, 1940]. The birds were not injected until at least 30 days after operation, and as many died meanwhile few were available for investigation.In the male birds the left testis was removed before the injections were started to serve as a control to the right testis which was removed at the end of the experi¬ ment. The testes were weighed after fixation and transference to 70 % alcohol.In the females ovarian conditions were determined by examination at laparotomy. RESULTSEffects of testosterone on the atrophie testis Testosterone propionate was injected daily (2 mg. in 0-5 ml. of sesame oil) into eight birds for 7-21 days starting 30 days after hypophysectomy. The right testis was removed on the day after the last injection and compared with the left testis which had been removed on the day preceding the first injection. Four birds survived the full course of twenty-one injections, the others dying after seven, eight and twelve injections. Three hypophysectomized birds had their testes removed at similar times though receiving no injections.The results given in Table 1 show that there was no significant difference between the weights of the right and left testes of the control birds, while in all cases but one

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confidence: 85%

Gonad Stimulation by Androgens in Hypophysectomized Pigeons

Chu¹,

You²

1944

Journal of Endocrinology

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“…The quantitative data obtained in the present series of hypoph} r sectomized rats demonstrated that young spermatids are always present, although in small number, in some cross sections of seminiferous tubules, i.e., those which are at stages I-VII of the cycle. Cutuly and Cutuly (1940) studying the endocrine control of the seminiferous epithelium divided spermatogenesis into three phases, a premeiotic, a reduction division phase, and a post-meiotic phase. According to these authors "the first phase seems to occur independently of any known hormonal stimulation, the second seems to be controlled by hypophyseal gonadotropin and the third is either independent of any recognized hormonal stimulation or else it progresses under the influence of testicular hormone."…”

Section: Degeneration Taking Place During Spermiogenesismentioning

confidence: 99%

Quantitative Study of Spermatogenesis in the Hypophysectomized Rat

1955

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“…Steinberger and Nelson (1955), using very large doses of oestradiol (222/kg/day) or stilboestrol (1 mg/day), which would completely suppress pituitary gonadotropins (Steinbergcr and Duckett 1967), observed quantitative spermatogenic arrest similar to that observed after hypophysectomy. Steinberger and Duckett (1967) have proposed that the qualitative progress of spermatogenesis is relatively independent of hormonal control up to the late pachytene stage of the primary spermatocytes, but that the reduction division requires testosterone rather than gonadotropic hormones as suggested by Cutuly and Cutuly (1940). Previous work (Elkington and Blackshaw 1970) has shown that testosterone and pregnant mare serum gonadotropin prevent the decline in enzymatic activity and qualitative histology of the oestrogen-treated rat testis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Oestradiol-3,17ß Dipropionate on Spermatogenesis and Lysosomal Enzymes in the Rat Tests

Elkington¹,

Blackshaw²

1971

Aust. Jnl. Of Bio. Sci.

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Daily intramuscular injections (20 f-Lgjday) of oestradiol-3, 17,8 dipropionate in adult male rats depressed body growth and reduced the weight of the testes and seminal vesicles between 4 and 8 days of treatment.The proportion of soluble acid phosphatase (E.C. 3.1.3.2), acid proteinase (E.C. 3.4.4.23), and ,8-glucuronidase (E.C. 3.2.1.31), increased during oestradiol treatment. There was also an increase in the total amount of acid proteinase and ,8.glucuronidase but no significant change in total acid phosphatase activity with oestrogen treatment. These changes in hydrolase activity coincided with a decrease in the yield of late primary spermatocytes from early primary spermatocytes, and long spermatidsfromroundspermatids. Histochemical evidence supported the biochemical and histochemical findings, with increasing tubular staining of acid phosphatase and lipid in the testis during treatment.It is concluded that lysosomal enzymes are important in testicular degeneration and probably in normal spermatogenesis.

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Observations on Spermatogenesis in Rats

Cited by 24 publications

References 0 publications

Gonad Stimulation by Androgens in Hypophysectomized Pigeons

Gonad Stimulation by Androgens in Hypophysectomized Pigeons

Quantitative Study of Spermatogenesis in the Hypophysectomized Rat

Effect of Oestradiol-3,17ß Dipropionate on Spermatogenesis and Lysosomal Enzymes in the Rat Tests

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