Observations on Pregnancy in Intact Rabbits and in Ovariectomized Rabbits Treated With 17 hydroxyprogesterone Caproate

Pickworth, Stella; Lamming, G. E.

doi:10.1530/jrf.0.0130457

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…Consistent with observations in mouse [36], sheep [18,45], and human [14], OPN mRNA levels are highest during the secretory phase, corresponding to the time of epithelial differentiation under the influence of progesterone. As demonstrated in the human [46], progesterone is essential for successful pregnancy in the rabbit [47]. Further, the rabbit uterus has been shown to be receptive to embryo attachment 5-7 d postovulation [48].…”

Section: Discussionmentioning

confidence: 94%

Regulated Expression of Osteopontin in the Peri-Implantation Rabbit Uterus1

Apparao

Illera

Beyler

et al. 2003

Biology of Reproduction

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Blastocyst attachment to the lining of the mammalian uterus during early implantation involves the initial apposition of the trophoblast to the uterine epithelial surface. Osteopontin (OPN) is a glycoprotein component of the extracellular matrix that is secreted by the glandular epithelium of mammalian uteri at the time of implantation. This protein is recognized by several members of the integrin family and promotes cell-cell attachment and adhesion. In the present study, rabbit uteri were examined using Northern and in situ hybridization to evaluate the temporal and spatial distribution of OPN mRNA during early pregnancy. Northern blot analysis demonstrated a dramatic increase in OPN expression on Days 4-7 of pregnancy, corresponding to the rise in circulating progesterone and the time of initial embryo attachment in this species. In situ hybridization analysis revealed OPN mRNA expression on Day 6.75 of pregnancy, which was most prominent on endometrial epithelium. Using immunofluorescence, OPN protein was present on the glandular epithelium on Day 6.75 of pregnancy, but was absent on blastocysts. Further, no expression of OPN mRNA or protein was found in the nonpregnant endometrium. Induction of endometrial OPN expression was observed in unmated rabbits treated with progesterone alone and was prevented by cotreatment with the antiprogestin ZK137.316. Estradiol-17beta had no effect on OPN expression by itself, and estrogen priming was not necessary to demonstrate the stimulatory effect of progesterone. In The rabbit uterus, as in other mammalian species studied, OPN is expressed in a stage-specific manner by the endometrial glands during the peri-implantation period and is regulated by progesterone.

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Section: Discussionmentioning

confidence: 94%

Regulated Expression of Osteopontin in the Peri-Implantation Rabbit Uterus1

Apparao

Illera

Beyler

et al. 2003

Biology of Reproduction

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“…In the rat indomethacin can completely inhibit implant¬ ation (Kennedy, 1977), but in rabbits there is only a reduction in implantation sites and live fetuses (Hoffman, 1978). However implantation in the rabbit can occur in the absence of oestrogen (Wu & Allen, 1959;Pickworth & Lamming, 1967), and oestrone and oestradiol levels do not change between ovulation and implantation but remain at low levels (Challis et al 1973;Spilman, Shaikh & Harper, 1978;Harper et al 1979). This is not true for rats (Canivenc et al 1956;Psychoyos, 1960;Shaikh, 1971), and hence an action of indomethacin on implantation mediated by the steroid hormone receptors in this latter species may be possible.…”

Section: Discussionmentioning

confidence: 99%

Progesterone and oestradiol receptor concentration and translocation in uterine tissue of rabbits treated with indomethacin

Cao¹,

Jones²,

Harper³

1985

Journal of Endocrinology

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The purpose of this study was to determine whether endogenous prostaglandins are involved in the regulation of sexual steroid receptor concentration and cytosol to nucleus translocation in the rabbit uterus. The animals were injected s.c. with indomethacin (10 mg/kg) twice daily on days 5 and 6 and on the morning of day 7 of pregnancy or pseudopregnancy and killed 2 h after the last injection. The indomethacin treatment did not change progesterone and oestradiol serum concentrations compared with animals injected with vehicle only. No differences were observed either in progesterone receptor or oestradiol (pregnant animals only) receptor concentration and intracellular distribution in endometria of indomethacin-treated or control animals. In an extended experiment indomethacin given to oestrogen-pretreated ovariectomized rabbits did not inhibit the nuclear progesterone receptor accumulation induced by a single progesterone injection. These results suggest that there is no direct relationship between prostaglandins and sexual steroid receptors in the rabbit uterus.

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“…The ovary is the main secretory source of progestin and estrogen and is indispensable to maintenance of pregnancy throughout gestation in many mammals, including cows (Estergreen et al 1967), goats (Meites et al 1951), dogs (Verstegen-Onclin & Verstegen 2008), and/or rabbits (Pickworth & Lamming 1967). On the other hand, an ovariectomy at mid-pregnancy or the later trimester of pregnancy does not disrupt the process of pregnancy in several mammals, including humans (Csapo et al 1972), monkeys (Albrecht et al 2000), guinea pigs (Bland & Donovan 1969), and/or mares (Hinrichs et al 1987), whose progestin and estrogen are supplied by other tissues such as the placenta.…”

Section: Introductionmentioning

confidence: 99%

The complete control of murine pregnancy from embryo implantation to parturition

Terakawa¹,

Watanabe²,

Obara³

et al. 2012

Reproduction

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The ovary is the main secretory source of progestin and estrogen and is indispensable to the maintenance of all events of pregnancy in mice. The purpose of this study was to control all processes of pregnancy in mice, from embryo implantation to parturition, without ovaries. The ovaries were removed before embryo implantation, and a single injection of medroxyprogesterone acetate (MPA) was given. Embryo implantation was induced by leukemia inhibitory factor, which can substitute 17b-estradiol (E 2 ). Continuous exposure to E 2 was necessary at mid-pregnancy, when placentation was completed. All mice sustained pregnancy without ovaries before parturition, which was initiated by the removal of E 2 and MPA. Murine pregnancy is a complicated process involving embryo implantation, placentation, and parturition. Complete control of pregnancy was achieved with the simple treatment of MPA and E 2 after induction of embryo implantation. Here, time-dependent events in the uterus during pregnancy could be realized without the ovaries, because the initiation of each event could be stringently controlled by hormonal treatments.

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Observations on Pregnancy in Intact Rabbits and in Ovariectomized Rabbits Treated With 17 hydroxyprogesterone Caproate

Abstract: Summary. Rabbits

Cited by 9 publications

References 8 publications

Regulated Expression of Osteopontin in the Peri-Implantation Rabbit Uterus1

Regulated Expression of Osteopontin in the Peri-Implantation Rabbit Uterus1

Progesterone and oestradiol receptor concentration and translocation in uterine tissue of rabbits treated with indomethacin

The complete control of murine pregnancy from embryo implantation to parturition

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