Observations on micronuclei ultrastructure within broad bean(Vicia faba) meristem after .GAMMA. ray radiation.

Soran, Viorel; Spârchez, Constanta; Crăciun, C.; Uray, Z

doi:10.1508/cytologia.46.381

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…The lagging chromosomes also contribute to the formation of the micronuclei, in addition to the acentric pieces. The heterochromatic micronuclei, created by acentric chromosomal fragments, and the euchromatic micronuclei, formed by one or more lagging chromosomes, were the two forms of micronuclei that Soren et al (1981) observed. There have been several theories as to why micronuclei originate and appear at telophases.…”

Section: Discussionmentioning

confidence: 89%

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.

Salim,

Bekhit,

Soudy

2023

Journal of Agricultural Chemistry and Biotechnology

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One of the excellent plant models for identifying various chromosomal abnormalities is Allium cepa L. In order to create genetic diversity, chemical mutagens such as; Ethyl methanesulfonate (EMS), Ethidium bromide (EtBr) and Sodium azide (SA) were used to mutagenize the plant's germplasm. The present study was carried out to determine how these chemicals affected the Mitotic Index (MI) and phase, chromosomal abnormalities, and frequency of micronuclei in the root tip cells of A. cepa Mutagens, in general, alter the genetic structure of the organisms, which is reflected in changes to a variety of features. Further, the genome's sensitivity was discovered through cytological examination when the three mutagens were used. The chromosomal entity was impacted by a variety of clastogenic and nonclastogenic alterations, as well as mitodepressive effects. The increase in dose/concentration of all the mutagens was shown to increase the frequency of both mitotic aberrant cells and mitotic chromosomal abnormalities in the treated root tip cells. Both chemical mutagens were shown to be less effective than EtBr at disrupting mitotic cell activity and primarily causing clastogenic chromosomal abnormalities. Numerous chromosomal aberrations, including stickiness and clumping of chromosomes with varying frequencies, fragments, laggards, bridges, precocious movements, micronuclei formation at telophases, and multipolar anaphases and telophases, were reported to be present in the mitotic aberrant cells. Anaphases, telophases, disorganised metaphases, desynchronized metaphases, and persisting nucleoli during anaphase were all noted.

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Section: Discussionmentioning

confidence: 89%

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.

Salim,

Bekhit,

Soudy

2023

Journal of Agricultural Chemistry and Biotechnology

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“…Several authors (Das et al, 1968;Bennett and Jellings, 1975;El-Bayoumi et al, 1979;Hesemann and Fayed, 1982) found micronuclei in untreated controls of various plant species. AIthough the production of micronuclei in root meristemcells is well established (Soran et al 1981;Degrassi and Rizzoni, 1982;Fayed andHesemann, 1983 andTang et al (1984), similar studies in meiotic divisions are relatively few. Das et al 1968;Reddy, 1971 andMikhael, 1972 observed micronuclei in PMC's of Vicia faba originated from seeds treatment with mutagenic agent.…”

Section: Introductionmentioning

confidence: 98%

Micronuclei, as Indicator of Mutagenecity In Cultivated Allium

Soliman¹

1999

Pakistan J. of Biological Sciences

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This study was performed to determine the relationship between chromosome aberrations in PMCs and micronuclei formation as well as chiasma frequency in cultivated Allium cepa collected from two sites. One site adjacent to the high way which is characterize by heavy traffic and the plants grown exposed to the different components of ignition exhaust in air as well as in soil, The other site away from the traffic pollution as control samples. Different types of chromosomal aberrations observed in first and second meiotic cells were: bridge, stickiness, chromosome aggregates in the form of a ring, vaculated nucleus, misorientation and multinucleated cells. Micronuclei formation represented the most dominant type of mieiotic abnormalities, The results indicated that aberration percentages of meiosis I were more frequent than those of meiosis II. Also a decrease in chiasma frequency in site I over site II was observed.

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Observations on micronuclei ultrastructure within broad bean(Vicia faba) meristem after .GAMMA. ray radiation.

Cited by 2 publications

References 11 publications

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.

Micronuclei, as Indicator of Mutagenecity In Cultivated Allium

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