Observations on house fly larvae midgut ultrastructure after Brevibacillus laterosporus ingestion

Ruiu, Luca; Satta, Alberto; Floris, Ignazio

doi:10.1016/j.jip.2012.08.005

Cited by 35 publications

(24 citation statements)

References 35 publications

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“…Since this protein has a significantly higher molecular mass compared to the other three, we cannot exclude that further differences may arise in assays employing these proteins in a similar molar concentration. Previous studies documented the ultrastructural changes caused by B. laterosporus UNISS 18 spores to the midgut epithelium of flies18, thus supporting a toxin mediated process reminiscent of the disruptive action induced by diverse bacterial insecticidal proteins67. Accordingly, SC-CSPB proteins might be directly involved in the interaction with epithelial cells.…”

Section: Discussionmentioning

confidence: 78%

“…According to previous studies indicating the spore as the main active ingredient of B. laterosporus- based insecticidal preparations against the house fly17, our results imply that the spore holds specific toxic factors against this insect species. In fact, the death of flies ingesting spores, normally occurs before spore germination18. It is well documented that the variations in outer spore envelope composition of different bacterial species reflect adaptations to specific ecological niches1.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the spore surface layers, including the canoe-shaped parasporal body, are supposed to provide both mechanical and chemical resistance to the spore core, thus hindering lysozyme action and the other immune-related defence mechanisms encountered during the passage through the house fly’s intestinal tract18, and enabling the spore to maintain its pathogenic potential22. According to this scenario, an abundant expression of the spore coat genes would be critical for a proper formation of this envelope35.…”

Section: Discussionmentioning

confidence: 99%

“…Pathogenicity against dipterans, including mosquitoes and flies, has been associated with the ingestion of sporulated cultures with or without parasporal bodies13141516. Despite the possible implication of spore surface proteins in the toxic interaction with the midgut of house flies, the virulence factors acting against these insects have not been identified1718.…”

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confidence: 99%

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Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

Marche

Mura

Falchi

et al. 2017

Sci Rep

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Outer spore envelope proteins of pathogenic bacteria often present specific virulence factors and tools to evade the defence system of their hosts. Brevibacillus laterosporus, a pathogen of invertebrates and an antimicrobial-producing species, is characterised by a unique spore coat and canoe-shaped parasporal body (SC-CSPB) complex surrounding the core spore. In the present study, we identified and characterised major proteins of the SC-CSPB complex of B. laterosporus, and we investigated their entomopathogenic role. Employing a proteomic approach and a B. laterosporus-house fly study model, we found four highly conserved proteins (ExsC, CHRD, CpbA and CpbB) that function as insect virulence factors. CpbA was associated with a significantly higher mortality of flies and greater relative gene expression levels during sporulation, compared to the other SC-CSPB proteins. Taken together, we suggest that spore surface proteins are a part of a complex set of toxins and virulence factors that B. laterosporus employs in its pathogenicity against flies.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

Marche

Mura

Falchi

et al. 2017

Sci Rep

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“…The search was performed against all genomes available in the NCBI microbial genome database except for the B. cereus group and Clostridia (2139 genomes on 01/02/2013). A cluster of spsRKABC orthologous genes was identified in two other organisms: Brevibacillus laterosporus LMG 15441 and Paenibacillus dendritiformis C454 (E-value 0.0) which are sporeforming bacteria found associated with invertebrate guts 23 . In many organisms, including Gram-negative bacteria such as the gut bacteria Vibrio cholerae , spsC -orthologs were found.…”

Section: Discussionmentioning

confidence: 99%

An insect gut environment reveals the induction of a new sugar-phosphate sensor system inBacillus cereus

et al. 2013

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Bacteria survive under various conditions by sensing stimuli triggering specific adaptive physiological responses, which are often based on membrane-integrated sensors connected to a cytoplasmic regulator. Recent studies reveal that mucus glycans may act as signal molecules for two-component systems involved in intestinal colonization. Bacillus cereus, a human and insect opportunistic pathogen was used to identify bacterial factors expressed in an insect gut infection model. The screen revealed a promoter involved in the expression of a gene with so far unknown functions. A search for gut-related compounds, inducing its transcription, identified glucose-6-phosphate as an activation signal. The gene is part of a five-gene cluster, including a two-component system. Interestingly such five gene loci are conserved in the pathogenic Bacillus group as well as in various Clostridia bacteria and are with analogy to other multi-component sensor systems in enteropathogenic bacteria, such as E. coli. Thus our results provide insights into the function of two-component and auxiliary sensor systems in host-microbe interactions and opens up possible investigations of such systems in other gut associated bacteria.

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Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

Pereira¹,

Queiroz

Faria

et al. 2021

Microscopy Res & Technique

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Brevibacillus laterosporus has entomopathogenic potential against several orders of insects and its wide bioactivity is associated with a variety of strain-specific molecules.In order to avoid the use of synthetic insecticides, along with the need to control insect pests, microbial control has been widely used. Muscoid dipterans are known for their medical-veterinary and sanitary importance, and synanthropy. The enormous biotechnological potential of B. laterosporus has been demonstrated, but there are still few studies with muscoid dipterans. The aim of the study was to verify the mortality of B. laterosporus NRS590 on synanthropic flies and to characterize its different cell stages ultrastructurally. The flies were collected from garbage bins and the colonies were adapted to the laboratory conditions. Bioassays with neo larvae were carried out from the bacterial growth in the phases: vegetative (6 hr), sporangium (20 hr), and free spores (44 hr). An aliquot of each phase was collected for Transmission (TEM) and Scanning Electron Microscopy (SEM). The effectiveness of NRS590 was observed in the sporulation phase, where the corrected mortality was 83.3, 85.1, and 99% for Chrysomya megacephala, Chrysomya putoria, and Musca domestica, respectively. The parasporal body was observed in detail on the entire spore surface. Although our knowledge of this bacterium is growing, it remains to be determined the real virulence factors responsible for the wide entomopathogenic activity observed on muscoid dipterans. Therefore, this study can provide subsidies for the improvement of efficient and safe microbial control techniques for the environment and living beings.

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Observations on house fly larvae midgut ultrastructure after Brevibacillus laterosporus ingestion

Cited by 35 publications

References 35 publications

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

An insect gut environment reveals the induction of a new sugar-phosphate sensor system inBacillus cereus

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

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