Observations on function, composition, and structure of cestode calcareous corpuscles

Brand, Theodor von; Mercado, Teresa I.; Nylen, Marie U.; Scott, David B.

doi:10.1016/0014-4894(60)90026-6

Cited by 68 publications

(19 citation statements)

References 8 publications

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“…They also may prove to have some relationship to the so called "calcareous corpuscles" of tapeworms which have been isolated and studied by yon Brand and his colleagues (29,30). Thus the deposition of the massive amounts of calcium phosphate by isolated mitochondria as reported in this study may be representative of a wide-spread biological capability elicited by specific physiological conditions.…”

Section: Fmnae 15 Loaded Mitochondria Containing (Lense Granules (Dg)supporting

confidence: 51%

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Greenawalt

Rossi

Lehninger

1964

The Journal of Cell Biology

356

126

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Rat liver mitochondria allowed to accumulate maximal amounts of Ca ++ and HPO4 = ions from the suspending medium in vitro during respiration have a considerably higher specific gravity than normal mitochondria and may be easily separated from the latter by isopycnic centrifugation in density gradients of sucrose or cesium chloride. When the mitochondria are allowed to accumulate less than maximal amounts of Ca++ and HPO4= from the medium, they have intermediate specific gravities which are roughly proportional to their content of calcium phosphate. Maximally "loaded" mitochondria are relatively homogeneous with respect to specific gravity. Correlated biochemical and electron microscopic studies show that Ca++-loaded mitochondria contain numerous dense granules, of which some 85 per cent are over 500 A in diameter. These granules are electron-opaque not only following fixation and staining with heavy metal reagents, but also following fixation with formaldehyde, demonstrating that the characteristic granules in Ca++-loaded mitochondria have intrinsic electron-opacity. The dense granules are almost always located within the inner compartment of the mitochondria and not in the space between the inner and outer membranes. They are frequently located at or near the cristae and they often show electrontransparent "cores." Such granules appear to be made up of clusters of smaller dense particles, but preliminary x-ray diffraction analysis and electron diffraction studies have revealed no evidence of crystallinity in the deposits. The electron-opaque granules decrease in number when the Ca++-loaded mitochondria are incubated with 2,4-dinitrophenol; simultaneously there is discharge of Ca ++ and phosphate from the mitochondria into the medium.

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Section: Fmnae 15 Loaded Mitochondria Containing (Lense Granules (Dg)supporting

confidence: 51%

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Greenawalt

Rossi

Lehninger

1964

The Journal of Cell Biology

356

126

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“…According to Martin and Bils (1964) modified mitochondria and possibly cytoplasmic membranes together with electron dense deposits constitute the centres or "nuclei" around which the concretion material becomes organised into concentric layers which were composed mainly of calcium carbonate and a trace of phosphate. Calcareous excretory concretions have also been deposited as concentric lamellae in eestodes (yon Brand et al, 1960).…”

Section: Fine Structure Observationsmentioning

confidence: 98%

Histochemical and fine structure observations of the intestinal epithelium ofTrichuris suis (Nematoda: Trichuroidea)

Jenkins¹

1973

Z. F. Parasitenkunde

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“…In particular, the proteins are regarded as important components of the structural framework or to act as a substrate for mineral deposition (Nieland and von Brand, 1969). The presence of corpuscular proteins was first demonstrated in worm sections by histochemical staining (Chowdhury et al, 1955;von Brand et al, 1960), and purified corpuscles showed protein binding activities against crude extracts of Platyhelminthes (Yang, 2000). However, no reports are available on the protein profiles of calcareous corpuscles.…”

mentioning

confidence: 99%

“…Two protein molecules, at 21 and 16 kDa, were particularly intense. It is known that the calcareous corpuscles of cestodes contain relatively little protein as compared with their inorganic and other organic components (von Brand et al, 1960;von Brand and Nylen, 1970). The amount of proteins in dried corpuscles of Echinococcus granulosus was estimated to be about 4.8-7.8% (von Brand et al, 1965).…”

mentioning

confidence: 99%

“…Several suggestions have been made on possibilities about their functions, but their exact roles remain unclear. The main components of calcareous corpuscles are inorganic in nature, e.g., calcium and magnesium, and phosphates and carbonates (von Brand et al, 1960). The organic matrix of calcareous corpuscles is composed of carbohydrates, lipids, and relatively few proteins (von Brand and Nylen, 1970).…”

mentioning

confidence: 99%

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A new method for concentration of proteins in the calcareous corpuscles separated from the spargana of Spirometra erinacei

Park

Guk

et al. 2005

Korean J Parasitol

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Calcareous corpuscles are a characteristic structure found in larval and adult stage cestodes. These corpuscles are known to contain several protein components and to possess protein-binding activity. However, the proteins bound to calcareous corpuscles in situ have not been studied. The present study was undertaken to identify the proteins on calcareous corpuscles. Calcareous corpuscles were purified from the plerocercoids (= spargana) of Spirometra erinacei, and serially dissolved using 0.1 M sulfamic acid solution. Collected supernatants were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. The results showed that only the fraction remaining after the 19th dissolved fraction contained proteins. A total of 20 protein molecules were detected in gel, with major bands at 56, 53, 46, 40, 35, 29, 28, 24.5, 21, 19, 16, 13, 10 and 8 kDa. In particular, the proteins corresponding to the 21 and 16 kDa bands were most abundant. Our results demonstrated for the first time the protein contents of the calcareous corpuscles of spargana. Further studies on the functions of these proteins are required.

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Observations on function, composition, and structure of cestode calcareous corpuscles

Cited by 68 publications

References 8 publications

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Histochemical and fine structure observations of the intestinal epithelium ofTrichuris suis (Nematoda: Trichuroidea)

A new method for concentration of proteins in the calcareous corpuscles separated from the spargana of Spirometra erinacei

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