Observations on an enzymic method for the estimation of pyruvate in blood

Gloster, J; Harris, Peter

doi:10.1016/0009-8981(62)90011-6

Cited by 74 publications

(29 citation statements)

References 10 publications

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“…The explanation of the high ratio in the present study is to be found in the low resting concentration of pyruvate. Gloster and Harris (15) (34,35), but in both cases the subjects were postabsorptive and ambulatory, and the results of this study indicate a prolonged elevation of pyruvate levels after even mild degrees of exercise. Krasnow, Neill, Messer, and Gorlin (36) found a wide range of lactate-pyruvate ratios in arterial blood among a group of six resting normal subjects; the ratio varied from 3: 1 to 13: 1.…”

Section: Methodsmentioning

confidence: 57%

Lactate and pyruvate metabolism in the exercising ischemic limb.

Pentecost¹,

Reid²,

Reid³

1966

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 57%

Lactate and pyruvate metabolism in the exercising ischemic limb.

Pentecost¹,

Reid²,

Reid³

1966

J. Clin. Invest.

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“…Ferricyanide was used as an electron acceptor to avoid measurement of the later oxidative steps in the pyruvate dehydrogenase complex (24). The concentration of pyruvic acid, 0.2 mmoles/liter, was about twice that in normal blood under basal conditions (12).…”

Section: Metabolic Studiesmentioning

confidence: 80%

A defect in pyruvate decarboxylase in a child with an intermittent movement disorder

Blass¹,

Avigan²,

Uhlendorf³

1970

J. Clin. Invest.

246

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“…Filtrates were subsequently analyzed for ATP (6), adenosine diphosphate (ADP) (7), lactate (8), glucose (9), 2,3-DPG (10), glyceraldehyde-3-phosphate (G3P) (11), dihydroxyacetone phosphate (DHAP) (11), fructose diphosphate (FDP) (11), and 3-phosphoglycerate (3PG) (11). Pyruvate cannot be recovered adequately from red cells (12). Therefore, cell suspensions were rapidly centrifuged at 4°C and filtrates prepared from the buffer, as described above.…”

Section: Methodsmentioning

confidence: 99%

Energy metabolism in human erythrocytes

Feig¹,

Segel²,

Shohet³

et al. 1972

J. Clin. Invest.

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A B S T R A C T Normal red cells were incubated in the absence of glucose to develop a system in which total adenosine triphosphate (ATP) turnover could be assessed. After 1 hr, the triose pool had been completely consumed. Thereafter, the metabolism of 2,3-diphosphoglycerate (DPG) to pyruvate and lactate was the sole significant source of ATP synthesis.1-'M CuC12, which did not enter the cells, diminished ATP utilization by more than 50%. This could be only partially attributed to the inhibition by copper of residual acylation and cation pumping, which were already reduced by glucose depletion. Other membrane enzymes, which presumably function in the maintenance of membrane integrity, must, therefore, use a significant portion of erythrocyte ATP.The behavior of glucose-depleted red cells with respect to cation transport was complex. The addition of ouabain did not decrease ATP utilization in these red cells. Ouabain inhibitable potassium influx was nearly normal after triose depletion, but total potassium influx was decreased. In contrast, the ouabain inhibitable sodium efflux was markedly reduced after triose depletion, although the concentration of ATP was 70% of normal. The dissociation of monovalent cation pumping suggests that the energy for active sodium transport is derived from a specific source (such as the ATP produced by the phosphoglycerate kinase reaction) distinct from that for potassium transport.

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Observations on an enzymic method for the estimation of pyruvate in blood

Cited by 74 publications

References 10 publications

Lactate and pyruvate metabolism in the exercising ischemic limb.

Lactate and pyruvate metabolism in the exercising ischemic limb.

A defect in pyruvate decarboxylase in a child with an intermittent movement disorder

Energy metabolism in human erythrocytes

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