Observations of Variable Ammonia Oxidation and Nitrous Oxide Flux in a Eutrophic Estuary

Laperriere, Sarah M.; Nidzieko, Nicholas J.; Fox, Rebecca J.; Fisher, Alexander W.; Santoro, Alyson E.

doi:10.1007/s12237-018-0441-4

Cited by 25 publications

(24 citation statements)

References 80 publications

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“…In the estuary, nitrification rates in 2016 ranged from undetectable to 79.4 ± 0.02 nmol L −1 d −1 and increased along the salinity gradient ( Figure 2B). These rates are similar to those measured in other coastal marine systems (Horak et al, 2013;Damashek et al, 2016;Heiss and Fulweiler, 2016;Laperriere et al, 2018). Undetectable and low nitrification rates in the upstream estuary are likely due to the presence of cyanoHABs during sampling and increased competition for NH 4 + .…”

Section: Discussion Nitrification During Cyanobacterial Bloomssupporting

confidence: 82%

“…A comprehensive review of nitrification in estuaries reported a trend of elevated nitrification rates at Estuary Turbidity Maxima (ETM; Damashek and Francis, 2018), some of which were also associated with turbid waters and strong wind events (Hsiao et al, 2014;Happel et al, 2018;Laperriere et al, 2018;Sanders et al, 2018). NH 4 + released from resuspended sediments can thus lead to nitrification "hot spots" (Percuoco et al, 2015;Damashek et al, 2016).…”

Section: Nitrification Following Hurricane Irmamentioning

confidence: 99%

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Hurricane Disturbance Stimulated Nitrification and Altered Ammonia Oxidizer Community Structure in Lake Okeechobee and St. Lucie Estuary (Florida)

et al. 2020

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Nitrification is an important biological link between oxidized and reduced forms of nitrogen (N). The efficiency of nitrification plays a key role in mitigating excess N in eutrophic systems, including those with cyanobacterial harmful algal blooms (cyanoHABs), since it can be closely coupled with denitrification and removal of excess N. Recent work suggests that competition for ammonium (NH 4 +) between ammonia oxidizers and cyanoHABs can help determine microbial community structure. Nitrification rates and ammonia-oxidizing archaeal (AOA) and bacterial (AOB) community composition and gene abundances were quantified in Lake Okeechobee and St. Lucie Estuary in southern Florida (United States). We sampled during cyanobacterial (Microcystis) blooms in July 2016 and August 2017 (2 weeks before Hurricane Irma) and 10 days after Hurricane Irma made landfall. Nitrification rates were low during cyanobacterial blooms in Lake Okeechobee and St. Lucie Estuary, while low bloom conditions in St. Lucie Estuary coincided with greater nitrification rates. Nitrification rates in the lake were correlated (R 2 = 0.94; p = 0.006) with AOA amoA abundance. Following the hurricane, nitrification rates increased by an order of magnitude, suggesting that nitrifiers outcompeted cyanobacteria for NH 4 + under turbid, poor light conditions. After Irma, AOA and AOB abundances increased in St. Lucie Estuary, while only AOB increased in Lake Okeechobee. AOA sequences clustered into three major lineages: Nitrosopumilales (NP), Nitrososphaerales (NS), and Nitrosotaleales (NT). Many of the lake OTUs placed within the uncultured and uncharacterized NS δ and NT β clades, suggesting that these taxa are ecologically important along this eutrophic, lacustrine to estuarine continuum. After the hurricane, the AOA community shifted toward dominance by freshwater clades in St. Lucie Estuary and terrestrial genera in Lake Okeechobee, likely due to high rainfall and subsequent increased turbidity and freshwater loading from the lake into the estuary. AOB community structure was not affected by the disturbance. AOA communities were consistently more diverse than AOB, despite fewer

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Section: Discussion Nitrification During Cyanobacterial Bloomssupporting

confidence: 82%

Section: Nitrification Following Hurricane Irmamentioning

confidence: 99%

Hurricane Disturbance Stimulated Nitrification and Altered Ammonia Oxidizer Community Structure in Lake Okeechobee and St. Lucie Estuary (Florida)

et al. 2020

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“…The estuary sediments also act as a source of N 2 O, which is released into the overlying waters through denitrification (Tan et al, 2019); however, in estuarine waters, nitrification apparently is the main source of N 2 O production. Previous studies also proposed that nitrification may be the major source of N 2 O production in the water column in estuarine systems, such as the Guadalquivir (Huertas et al, 2018), Scheldt (De Wilde and De Bie, 2000), and Chesapeake Bay (Laperriere et al, 2019). However, in the estuarine sediments, N 2 O production was attributed to both nitrification and denitrification, such as in the Tama River (Japan) (Usui et al, 2001) and Yangtze (China) estuaries (Liu et al, 2019;Wang et al, 2019), where denitrification is the major nitrogen removal pathway with N 2 O production and consumption.…”

Section: Contribution Of Nitrification Versus Denitrificationmentioning

confidence: 96%

“…In particular, eutrophic estuaries with extensive oxygen-deficient zones have been considered hotspot regions for N 2 O production (Abril et al, 2000;De Wilde and De Bie, 2000;Garnier et al, 2006;Lin et al, 2016), with oversaturated N 2 O and high N 2 O concentrations and flux (De Wilde and De Bie, 2000;De Bie et al, 2002;Garnier et al, 2006;Rajkumar et al, 2008;Barnes and Upstill-Goddard, 2011;Lin et al, 2016). The dynamics of N 2 O emissions in these ecosystems are regulated by complex physical and biogeochemical processes; for example, mixing between fresh water and oceanic waters influences the biogeochemistry of estuarine waters as well as microbial activity (Huertas et al, 2018;Laperriere et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Nitrification is often credited as the dominant N 2 O production pathway in estuaries (De Bie et al, 2002;Barnes and Upstill-Goddard, 2011;Kim et al, 2013;Lin et al, 2016;Huertas et al, 2018;Laperriere et al, 2019). Although AOA frequently outnumber AOB and dominate microbial communities, their contribution to nitrification remains controversial in estuarine and coastal waters (Bernhard et al, 2010;Zhang et al, 2014;Hou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Major role of ammonia-oxidizing bacteria in N<sub>2</sub>O production in the Pearl River estuary

Lin

Xie

et al. 2019

Biogeosciences

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Abstract. Nitrous oxide (N2O) has significant global warming potential as a greenhouse gas. Estuarine and coastal regimes are the major zones of N2O production in the marine system. However, knowledge on biological sources of N2O in estuarine ecosystems remains controversial but is of great importance for understanding global N2O emission patterns. Here, we measured concentrations and isotopic compositions of N2O as well as distributions of ammonia-oxidizing bacterial and archaeal amoA and denitrifier nirS genes by quantitative polymerase chain reaction along a salinity gradient in the Pearl River estuary, and we performed in situ incubation experiments to estimate N2O yields. Our results indicated that nitrification predominantly occurred, with significant N2O production during ammonia oxidation. In the hypoxic waters of the upper estuary, strong nitrification resulted in the observed maximum N2O and ΔN2Oexcess concentrations, although minor denitrification might be concurrent at the site with the lowest dissolved oxygen. Ammonia-oxidizing β-proteobacteria (AOB) were significantly positively correlated with all N2O-related parameters, although their amoA gene abundances were distinctly lower than ammonia-oxidizing archaea (AOA) throughout the estuary. Furthermore, the N2O production rate and the N2O yield normalized to amoA gene copies or transcripts estimated a higher relative contribution of AOB to the N2O production in the upper estuary. Taken together, the in situ incubation experiments, N2O isotopic composition and concentrations, and gene datasets suggested that the high concentration of N2O (oversaturated) is mainly produced from strong nitrification by the relatively high abundance of AOB in the upper reaches and is the major source of N2O emitted to the atmosphere in the Pearl River estuary.

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Ecological Role of Microbes

Turk

Malkin

Celussi

et al. 2020

Coastal Ecosystems in Transition

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Observations of Variable Ammonia Oxidation and Nitrous Oxide Flux in a Eutrophic Estuary

Cited by 25 publications

References 80 publications

Hurricane Disturbance Stimulated Nitrification and Altered Ammonia Oxidizer Community Structure in Lake Okeechobee and St. Lucie Estuary (Florida)

Hurricane Disturbance Stimulated Nitrification and Altered Ammonia Oxidizer Community Structure in Lake Okeechobee and St. Lucie Estuary (Florida)

Major role of ammonia-oxidizing bacteria in N<sub>2</sub>O production in the Pearl River estuary

Ecological Role of Microbes

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Observations of Variable Ammonia Oxidation and Nitrous Oxide Flux in a Eutrophic Estuary

Cited by 25 publications

References 80 publications

Hurricane Disturbance Stimulated Nitrification and Altered Ammonia Oxidizer Community Structure in Lake Okeechobee and St. Lucie Estuary (Florida)

Hurricane Disturbance Stimulated Nitrification and Altered Ammonia Oxidizer Community Structure in Lake Okeechobee and St. Lucie Estuary (Florida)

Major role of ammonia-oxidizing bacteria in N&lt;sub&gt;2&lt;/sub&gt;O production in the Pearl River estuary

Ecological Role of Microbes

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Major role of ammonia-oxidizing bacteria in N<sub>2</sub>O production in the Pearl River estuary