Observations of the spore, gametophyte and young sporophyte of Pteridium caudatum (L.) Maxon using scanning electron microscopy

Ramírez-Trejo, María del Rosario; Pérez-García, Blanca; Mendoza-Ruíz, Aniceto; Valdez-Avila, Ricardo; Espinosa‐Matías, Silvia

doi:10.1016/j.micron.2012.10.011

Cited by 8 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Graf-Meier (1985) studied Pteridium arachnoideum spores (under P. aquilinum) with LM. Furthermore, South American species of Pteridium were studied with SEM in some local palynological fl ora (Lorscheitter et al 2002, Contreras-Duarte et al 2006, Giudice et al 2006 as well as in morphologic studies on gametophyte (Ramírez-Trejo et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Palynological analysis of Dennstaedtiaceae taxa from the Paranaense Phytogeographic Province that produce Trilete spores II: Microlepia speluncae and Pteridium arachnoideum

Yañez

Márquez

Morbelli

2016

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The spore morphology and wall ultrastructure of Microlepia speluncae and Pteridium arachnoideum from the Paranaense Province were analyzed with LM, SEM and TEM and a comparative analysis was carried out. In both species the spores are covered by a three-dimensional network of threads branched and fused, tangentially arranged to the surface, and some free-end threads are also seen. The species were differentiated by morphology and the frequency of threads fusion and the networks distribution on the surface of the spores. In both species the exospore is two-layered in section, both layers are traversed by single or branched channels. The perispore is three-layered in section: the inner layer is adhered to the exospore, the middle layer is formed of a three-dimensional network of threads and the outer layer is discontinuous. The perispore ultrastructure of Microlepia speluncae was interpreted as formed of helical subunits displayed around a central channel. The spore morphology and perispore ultrastructure allow differentiating Microlepia from Pteridium but also to the other Dennstaedtiaceae genera that grow in the Paranaense Province. The results obtained allow establishing relationships that let us recognize different groups and gave a new reference to get a better knowledge of the family.

show abstract

Section: Introductionmentioning

confidence: 99%

Palynological analysis of Dennstaedtiaceae taxa from the Paranaense Phytogeographic Province that produce Trilete spores II: Microlepia speluncae and Pteridium arachnoideum

Yañez

Márquez

Morbelli

2016

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…El tiempo de germinación es semejante al reportado para otras especies de la familia, ya que en todas ocurre durante la primera semana después de la siembra (Campbell, 1936;Kaur, 1972;Zhang, Liu, & Tan, 2008;Ramírez-Trejo, Pérez-García, Mendoza-Ruiz, Valdéz-Ávila, & Espinosa-Matías, 2013;Martínez, Chambi, & Avilés, 2014). Sin embargo, en P. aquilinum el tiempo de germinación es menor con respecto a las especies que conforman la familia, a excepción de P. caudatum en el que se reporta a los dos días posteriores a la siembra (Ramírez-Trejo et al, 2013). La germinación de P. aquilinum es del tipo Vittaria al igual que en las especies ya antes estudiadas como: Dennstaedtia scabra (Wall.…”

Section: Discussionunclassified

“…(Zhang et al, 2008), M. strigosa (Thunb.) C. Presl (Zhang et al, 2008) y Pteridium caudatum (L.) Maxon (Ramírez-Trejo et al, 2013), todas incluidas en la familia Dennstaedtiaceae de acuerdo a la Smith et al (2006).…”

Section: Discussionunclassified

“…El desarrollo protálico es del tipo Adiantum al igual que el resto de las especies en la familia Dennstaedtiaceae (Campbell, 1936;Kaur, 1972;Zhang et al, 2008;Ramírez-Trejo et al, 2013;Martínez et al, 2014). Sin embargo, la morfogénesis de la fase sexual en esta especie, es más rápida que en el resto de los miembros de la familia, ya que a los 15 días en condiciones in vitro se observan gametofitos con anteridios y arquegonios.…”

Section: Discussionunclassified

See 1 more Smart Citation

Morfo-anatomía del ciclo de vida del helecho Pteridium aquilinum (Dennstaedtiaceae) en cultivo in vitro

Eslava-Silva

Durán

Jiménez-Estrada

et al. 2019

RBT

View full text Add to dashboard Cite

Introducción: Pteridium aquilinum es un helecho heliófilo ampliamente distribuido en México. Es una especie pionera que usualmente se encuentra en hábitats alterados y tiene gran relevancia ecológica por sus propiedades alelopáticas, su resistencia al fuego y a condiciones de sequía. Objetivo: realizar una descripción integral de la forma y ornamentación de las esporas de P. aquilinum, así como de la morfogénesis del gametofito, incluyendo el tipo de germinación y el desarrollo protálico (resaltando su morfología). Además, describir la anatomía del esporofito joven desarrollado en condiciones in vitro. Metodología: se utilizó microscopía electrónica de barrido y técnica histológica de parafina para la descripción de las fases del gametofito y esporofito. Resultados: Las esporas son globulares y el trilete tiene ornamentación granulada, mientras que su germinación fue de tipo Vittaria y el desarrollo protálico correspondió al tipo Adiantum. Los anteridios se observaron 13 días después de la siembra, mientras que los arquegonios surgieron al día 17. La primera hoja del esporofito apareció entre los días 60 y 70. Al cuarto mes, la fase esporofítica desarrollada in vitro mostró la anatomía típica de la especie a esta edad, dado que no presentó vitrificación. El análisis histológico del rizoma, la base del peciolo, el raquis de primer orden y la lámina mostraron los sistemas de tres tejidos bien diferenciados. Las modificaciones anatómicas observadas in vitro, como una dictiostela monocíclica y sólo una banda de esclerénquima en el rizoma, podrían atribuirse a la edad de los individuos. Por otra parte, los estomas están presentes en la superficie adaxial de la lámina, que corresponde al tipo anomocítico. Aunque estos estomas se formaron en condiciones in vitro, es importante resaltar que son completamente funcionales. Conclusiones: Nuestro trabajo describe por primera vez la morfo-anatomía de las fases gametofítica y esporofítica del ciclo de vida de P. aquilinum en condiciones in vitro. Nuestros resultados posiblemente permitan la exploración con mayor profundidad de las propiedades biológicas, fisiológicas y ecológicas de la especie.

show abstract

“…All previous works demonstrated excellent performance and have been conducted to obtain the shape features of the spores. Shape feature alone cannot be used for detection of rice blast and rice smut spores, due to the small size and similar structure of spores, which are easy to confuse with each other or environmental impurities at the time of detection. Therefore, texture features can be used to identify microscopic images.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of rice disease using microscopy image identification based on the synergistic judgment of texture and shape features and decision tree–confusion matrix method

et al. 2019

View full text Add to dashboard Cite

BACKGROUND Rice smut and rice blast are listed as two of the three major diseases of rice. Owing to the small size and similar structure of rice blast and rice smut spores, traditional microscopic methods are troublesome to detect them. Therefore, this paper uses microscopy image identification based on the synergistic judgment of texture and shape features and the decision tree–confusion matrix method. RESULTS The distance transformation–Gaussian filtering–watershed algorithm method was proposed to separate the adherent rice blast spores, and the accuracy was increased by about 10%. Four shape features (area, perimeter, ellipticity, complexity) and three texture features (entropy, homogeneity, contrast) were selected for decision‐tree model classification. The confusion‐matrix algorithm was used to calculate the classification accuracy, in which global accuracy is 82% and the Kappa coefficient is 0.81. At the same time, the detection accuracy is as high as 94%. CONCLUSIONS The synergistic judgment of texture and shape features and the decision tree–confusion matrix method can be used to detect rice disease quickly and precisely. The proposed method can be combined with a spore trap, which is vital to devise strategies early and to control rice disease effectively. © 2019 Society of Chemical Industry

show abstract

Observations of the spore, gametophyte and young sporophyte of Pteridium caudatum (L.) Maxon using scanning electron microscopy

Cited by 8 publications

References 41 publications

Palynological analysis of Dennstaedtiaceae taxa from the Paranaense Phytogeographic Province that produce Trilete spores II: Microlepia speluncae and Pteridium arachnoideum

Palynological analysis of Dennstaedtiaceae taxa from the Paranaense Phytogeographic Province that produce Trilete spores II: Microlepia speluncae and Pteridium arachnoideum

Morfo-anatomía del ciclo de vida del helecho Pteridium aquilinum (Dennstaedtiaceae) en cultivo in vitro

Rapid detection of rice disease using microscopy image identification based on the synergistic judgment of texture and shape features and decision tree–confusion matrix method

Contact Info

Product

Resources

About