Observations during in vitro growth of cells derived from rabbit kidney

Wallace, Roslyn E.; Orsi, Ernest V.; Ritter, Hilda B.; Moyer, Arden W.

doi:10.1016/0014-4827(61)90198-7

Cited by 7 publications

(1 citation statement)

References 47 publications

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“…During the past two decades, workers attempting to obtain suspensions of isolated kidney cells have employed a variety of methods for the disruption of the tissue. These methods include treatment with trypsin (Dulbecco & Vogt, 1954;Youngner, 1954;Fenje, 1960;Wallace et al, 1961;Phillips, 1967), Aspergillin-O (Sabina et al, 1963), tetraphenylboron (Rappaport & Howze, 1966) and various chelating agents (Kalant & Young, 1957;Rappaport & Howze, 1966), as well as the purely mechanical methods of forcing kidney tissue through progressively smaller needles (Zaroff et al, 1961) or a stainless-steel screen and pipette (Phillips, 1967(Phillips, , 1968. Thimmappayya et al (1970) used a modified Potter-Elvehjem homogenizer with a soft rubber pestle in their mechanical method for isolating kidney cells.…”

Section: Preparation and Some Properties Of A Suspension Of Fragmentedmentioning

confidence: 99%

Preparation and some properties of a suspension of fragmented tubules from rat kidney

Dawson

1972

Biochemical Journal

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A method is described whereby short fragments of rat kidney tubule were obtained when kidney slices were gently dispersed by exposure to collagenase and hyaluronidase. When suspended in buffered saline the fragmented tubules respired actively over a period of several hours, the rate of oxygen consumption being proportional to the amount of cell protein. Oxygen uptake was stimulated by the addition of glucose, lactate, butyrate, alpha-oxoglutarate and other substrates and was decreased by the omission of Ca(2+) from the suspending medium. With alpha-oxoglutarate as the added substrate, dinitrophenol strongly stimulated oxygen uptake. Dinitrophenol had a less-marked stimulatory effect when glucose was the added substrate, and inhibited respiration in the absence of added substrate. Oligomycin inhibited respiration and this inhibition was partially reversed by dinitrophenol. Fragmented tubules synthesized glucose from lactate at a high rate but this capacity for gluconeogenesis was abolished by dinitrophenol and by physically damaging the cells.

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Section: Preparation and Some Properties Of A Suspension Of Fragmentedmentioning

confidence: 99%

Preparation and some properties of a suspension of fragmented tubules from rat kidney

Dawson

1972

Biochemical Journal

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Feulgen-microspectrophotometric determination of different evolutionary patterns in serial cultures of cells derived from human skin tumours

Dewse¹,

Potter²

1971

European Journal of Cancer (1965)

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Changes in antigenic constitutions of embryonic chicken kidney cells during in vitro spreading culture

1965

Experimental Cell Research

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Observations during in vitro growth of cells derived from rabbit kidney

Cited by 7 publications

References 47 publications

Preparation and some properties of a suspension of fragmented tubules from rat kidney

Preparation and some properties of a suspension of fragmented tubules from rat kidney

Feulgen-microspectrophotometric determination of different evolutionary patterns in serial cultures of cells derived from human skin tumours

Changes in antigenic constitutions of embryonic chicken kidney cells during in vitro spreading culture

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