Observations by Light Microscopy on the Cytopathogenicity of Naegleria Fowleri in Mouse Embryo-Cell Cultures

Brown, Tim

doi:10.1099/00222615-11-3-249

Cited by 20 publications

(18 citation statements)

References 21 publications

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“…This was not thought to be due to increased resistance of the ME cells, although they were clearly affected by the drug and showed changes in morphology similar to those observed in mouse L-cells by Carter (1967). If, as previously suggested (Brown, 1978), the cytoplasmic processes of fibroblast-like cells are prone to attack by Naegleria trophozoites, the exaggerated protuberances of ME cells induced by cytochalasin B should have been especially susceptible to damage. Instead, the drug appeared to inhibit the cytopathogenicity of Naegleria by influencing trophozoite behaviour.…”

Section: Discussionmentioning

confidence: 99%

“…In cell cultures too, amoebae exhibit a pronounced cytopathic activity that is thought to correlate with pathogenicity in vivo (Culbertson, 197 1;Newton, 1972;Chang, 1973). Secondary mouse-embryo (ME) cells were recently shown to provide a sensitive in-vitro host system for the study of mechanisms of naegleria-induced mammalian-cell damage by techniques that are precluded in the intact animal (Brown, 1978). Observations of trophozoite behaviour in these cultures suggested that amoebae destroy mammalian cells only while in direct contact with them, rather than by secreting cytotoxic or cytolytic substances into the culture medium.…”

Section: Plate XXIIImentioning

confidence: 99%

“…processing of trophozoites for inoculation on to mammalian-cell monolayers and the preparation of secondary ME-cell cultures and associated media were as reported previously (Brown, 1978). Monolayers were established in 100 x 12-mm tissue-culture tubes and, for photomicrography, in disposable tissue-culture chambers (Sterilin Ltd, Richmond, Surrey; see Brown, 1978).…”

Section: Plate XXIIImentioning

confidence: 99%

“…Monolayers were established in 100 x 12-mm tissue-culture tubes and, for photomicrography, in disposable tissue-culture chambers (Sterilin Ltd, Richmond, Surrey; see Brown, 1978).…”

Section: Plate XXIIImentioning

confidence: 99%

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Inhibition by Amoeba-Specific Antiserum and by Cytochalasin B of the Cytopathogenicity of Naegleria Fowleri in Mouse Embryo-Cell Cultures

Brown

1979

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 99%

Section: Plate XXIIImentioning

confidence: 99%

Section: Plate XXIIImentioning

confidence: 99%

“…Monolayers were established in 100 x 12-mm tissue-culture tubes and, for photomicrography, in disposable tissue-culture chambers (Sterilin Ltd, Richmond, Surrey; see Brown, 1978).…”

Section: Plate XXIIImentioning

confidence: 99%

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Inhibition by Amoeba-Specific Antiserum and by Cytochalasin B of the Cytopathogenicity of Naegleria Fowleri in Mouse Embryo-Cell Cultures

Brown

1979

Journal of Medical Microbiology

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“…In the central nervous system, the amoebae multiply rapidly resulting in widespread hemorrhagic and nectrotizing encephalitis. The mechanisms underlying this cytopathogenicity of N. fowleri include trogocytosis (Brown 1978) and cytolytic substances released by the amoeba (Chang 1974). In addition to in vivo studies, trophozoites and lysates of amoebae were found to exert remarkable tissue-destructive activity in assays including cell-line cultures .…”

Section: Introductionmentioning

confidence: 99%

The involvement of an integrin-like protein and protein kinase C in amoebic adhesion to fibronectin and amoebic cytotoxicity

et al. 2004

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Adherence of a pathogen to the host cell is one of the critical steps in microbial infections. Naegleria fowleri, a causative agent of primary amoebic meningoencephalitis in humans, is expected to interact with extracellular components of the host, such as fibronectin, in a receptor-mediated mode. In this study, we investigated the interaction between N. fowleri and fibronectin to understand its cytopathology. In binding assays using immobilized fibronectin, the number of amoebae bound to fibronectin was increased compared to the controls, and was dependent on the amount of coated fibronectin present. A fibronectin binding protein of 60 kDa was found in extracts of N. fowleri. Western blot and immunolocalization assays using integrin a 5 /FnR antibodies showed that a 60 kDa protein reacted with the antibodies in extracts of N. fowleri, which was localized on the surface of N. fowleri. Preincubation of N. fowleri with the integrin antibodies significantly inhibited amoebic binding to fibronectin and cytotoxicity to the CHO cells. Additionally, protein kinase C activity was detected in the extract of N. fowleri. When N. fowleri was pretreated with protein kinase C activator or inhibitor, the abilities of amoebic adhesion to fibronectin and cytotoxicity to the host cells were markedly affected compared to untreated amoebae. These results suggest that an amoebic integrin-like receptor and protein kinase C play important roles in amoebic cellular processes in response to fibronectin.

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Cytopathogenicity ofNaegleria fowleri in mammalian cell cultures

John

1989

Parasitol Res

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A total of 13 strains of Naegleria fowleri were cytopathogenic for lung, kidney, foreskin, ovary, connective tissue, neuroblastoma, laryngeal carcinoma, and cervical carcinoma mammalian cell lines. The strains of N. fowleri varied considerably in their ability to produce a cytopathic effect (CPE). Likewise, the different mammalian cell lines exhibited varying degrees of susceptability to the cytopathogenicity of the amebae. The African green-monkey kidney (Vero) cell line proved to be useful for assessing the cytopathogenic potential of N. fowleri strains. Although one strain failed to produce CPE in Vero-cell cultures, it did so in the two neuroblastoma cell lines. Other factors affecting the extent of CPE produced were incubation temperature, ameba: mammalian cell ratio, and the length of time during which amebae were maintained in cell culture.

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Observations by Light Microscopy on the Cytopathogenicity of Naegleria Fowleri in Mouse Embryo-Cell Cultures

Cited by 20 publications

References 21 publications

Inhibition by Amoeba-Specific Antiserum and by Cytochalasin B of the Cytopathogenicity of Naegleria Fowleri in Mouse Embryo-Cell Cultures

Inhibition by Amoeba-Specific Antiserum and by Cytochalasin B of the Cytopathogenicity of Naegleria Fowleri in Mouse Embryo-Cell Cultures

The involvement of an integrin-like protein and protein kinase C in amoebic adhesion to fibronectin and amoebic cytotoxicity

Cytopathogenicity ofNaegleria fowleri in mammalian cell cultures

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