We report the first direct laboratory measurement of the spontaneous emission due to the hyperfine splitting of the ground state of a highly charged hydrogenlike ion excited by electron collisions. The transition between the F 4 and F 3 levels of the 1s 2 S 1͞2 configuration of hydrogenlike 165 Ho 651 was observed and its wavelength was determined to 5726.4 6 1.5 Å. After taking into account relativistic, nuclear charge distribution, Bohr-Weisskopf, and QED corrections, we observe a significant deviation from commonly tabulated values of the nuclear dipole magnetic moment of this nucleus. [S0031-9007(96)00712-0] PACS numbers: 32.10. Fn, 12.20.Fv, 21.10.Ky, 32.30.Jc Measurements of the hyperfine splitting of the ground state of hydrogen provide a very sensitive tool to explore QED and nuclear contributions to the electron energy. Hydrogenlike ions allow an extension into a region in which these contributions scale to larger values. The 1s electron of a highly charged ion probes the structure of the nucleus more deeply, due to its Coulomb field, enabling a very sensitive test of theory in strong fields. This method complements measurements of muonic atoms, where the muon probes the magnetic field distribution of the nucleus (Bohr-Weisskopf effect). While the spontaneous 1s hyperfine transition in H, D, and He 1 has been observed in radio astronomy, laboratory measurements in hydrogen and low-Z hydrogenlike ions have to rely on stimulated emission using a maser setup due to the extremely long lifetime of the upper hyperfine level (1.1 3 10 7 yr for H) [1,2]. This technique was successful in measuring the splitting in H, D, T, and He 1 . Laser pumping has recently been applied to H-like Bi 821 circulating at nearly 60% the speed of light in a heavy-ion storage ring [3] resulting in the first measurement of the hyperfine splitting in multiply charged ions. Here laser fluorescence was detected as the laser frequency was tuned in and out of resonance with the Doppler-shifted transition. Accurate a priori knowledge of the hyperfine transition is required, lest a prohibitively large scan in the laser frequency be performed.We measure the F 4 to F 3 hyperfine transition of the 1s ground level of H-like 165 Ho 661 using passive emission spectroscopy. The measured wavelength of 5726.4 Å differs 89 Å from a recent calculation [4]. The fact that the transition is excited by electron collisions and the relative ease of the present technique, in principle, opens the upper half of the periodic table to scientific scrutiny.Hydrogenlike holmium ions are produced and stored in a high energy electron beam ion trap (SuperEBIT) [15] by an electron beam of variable energy axially compressed by a high magnetic field. The SuperEBIT has been used to perform very accurate spectroscopic measurements of highly charged ions in the x-ray regime [6], while no such measurements have been made in the optical. The possibility of performing spectroscopy of highly charged ions in the visible on a low-energy EBIT was demonstrated only recently by M...