Observation of two-mode binding to DNA by bipyridyl-(ethylenediamine)platinum(II): Isothermal titrational calorimetry and midinfrared absorption studies

Szabó, Attila; Flowers, Robert A.; Carter, B. J.; Lee, S. A.

doi:10.1103/physreve.58.7754

Cited by 5 publications

(1 citation statement)

References 31 publications

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“…18 These generally are planar heterocyclic aromatic molecules, such as ethidium and daunomycin, 19 which are able to insert themselves between neighbouring DNA base pairs causing distortion of the sugarphosphate backbone at the intercalation site. 20 The intercalation process requires both electrostatic interactions of the positive charges of the compounds with the negatively charged phosphate oxygens of DNA and stacking between the aromatic rings of the intercalator and the DNA nucleobases. In the presence of intercalation processes in vivo inhibition of the nucleic acid synthesis is observed and the intercalating compounds show mutagenic, antibiotic, antibacterial and antitumor activity.…”

Section: Introductionmentioning

confidence: 99%

New mononuclear and homodinuclear Pt(ii) complexes with heterocyclic nitrogen chelates: Synthesis, characterization, intercalating ability and in vitro cytotoxic activity evaluation

et al. 2008

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A series of new mononuclear and dinuclear platinum(ii) compounds based on two bipyridyl systems, linked by an alkyl chain {1,2-bis[4-(4'-methyl-2,2'-bipyridinyl)]ethane, L2, (a), and 1,6-bis[4-(4'-methyl-2,2'-bipyridinyl)]hexane, L6, (b)} have been synthesized and characterized by IR and multinuclear and multidimensional NMR spectroscopy. The coordination sphere of the complexes, designed to give intercalating and/or covalent interactions with DNA, is completed only by exchangeable (Cl(-), I(-) or H(2)O) and/or not leaving (chelate ethylenediamine, en) saturating ligands. Quenching of the DNA-ethidium fluorescence was performed in order to verify the intercalating capability of the water soluble compounds. Furthermore, the in vitro cytotoxicity of all water soluble complexes has been assessed with respect to cisplatin on platinum-sensitive human endometrium (HeLa) and platinum-resistant human breast (MCF-7) cancer cell lines.

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Section: Introductionmentioning

confidence: 99%

New mononuclear and homodinuclear Pt(ii) complexes with heterocyclic nitrogen chelates: Synthesis, characterization, intercalating ability and in vitro cytotoxic activity evaluation

et al. 2008

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Experimental studies on the nature of bonding of DNA•bipyridyl-(ethylenediamme)platmum(II) and DNA•netropsin complexes in solution and oriented wet-spun films

Szabó

et al. 2002

Journal of Biomolecular Structure and Dynamics

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The stability of complexes of NaDNA with bipyridyl- (ethylenediamine)platinum(II) (abbreviated [(bipy)Pt(en)](2+)) and with netropsin has been studied using two techniques: (i) ultraviolet (UV) melting experiments were done on NaDNA* [(bipy)Pt(en)](2+), showing that the [(bipy)Pt(en)](2+) ligand stabilizes the DNA double helix structure; and (ii) swelling measurements (via optical microscopy) as a function of relative humidity were done on wet-spun oriented films of NaDNA*[(bipy)Pt(en)](2+) and of NaDNA*netropsin. The swelling data shows that an irreversible transition of the films occurs at high relative humidity, first for the NaDNA*netropsin, then for pure NaDNA, and lastly for the NaDNA*[(bipy)Pt(en)](2+). These results are indicative that the [(bipy)Pt(en)](2+) complex stabilizes the intermolecular bonds which mediate the film swelling characteristics. A model is suggested for the binding of [(bipy)Pt(en)](2+) to DNA to explain why the swelling experiments show this ligand as increasing the intermolecular bond strength between the DNA double helices, while netropsin decreases this degree of stabilization.

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An Ultraviolet Melting Study of the Stability of the DNA Double Helix in the NaDNA-bipyridyl-(ethylenediamine)platintum(II) Complex

Szabó

Lee

2008

Journal of Biomolecular Structure and Dynamics

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Complexes of NaDNA with the bipyridyl-(ethylenediamine)platintum(II) (abbreviated [(bipy)Pt(en)]2+) molecular ion have been studied in solution via ultraviolet absorption experiments at 260 nm between 50 and 90 degrees C. These measurements, performed as a function of the molar ratio of the [(bipy)Pt(en)]2+ complex to DNA base pairs, show that the stability of the DNA double helix is increased by the formation of the DNA.[(bipy)Pt(en)]2+ complex: at a molar ratio of 0.33, the temperature at which the DNA double helix separates into two single strands is increased by about 15 degrees C.

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Observation of two-mode binding to DNA by bipyridyl-(ethylenediamine)platinum(II): Isothermal titrational calorimetry and midinfrared absorption studies

Cited by 5 publications

References 31 publications

New mononuclear and homodinuclear Pt(ii) complexes with heterocyclic nitrogen chelates: Synthesis, characterization, intercalating ability and in vitro cytotoxic activity evaluation

New mononuclear and homodinuclear Pt(ii) complexes with heterocyclic nitrogen chelates: Synthesis, characterization, intercalating ability and in vitro cytotoxic activity evaluation

Experimental studies on the nature of bonding of DNA•bipyridyl-(ethylenediamme)platmum(II) and DNA•netropsin complexes in solution and oriented wet-spun films

An Ultraviolet Melting Study of the Stability of the DNA Double Helix in the NaDNA-bipyridyl-(ethylenediamine)platintum(II) Complex

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