Observation of trichomonads infection in a child with periodontitis by direct microscopy at the dental office

Marty, Mathieu; Bonner, Mark; Vaysse, Frédéric

doi:10.1017/s0031182015000876

Cited by 5 publications

(5 citation statements)

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“…There is a growing body of evidence indicating the association of human oral protists and their potential contribution to periodontal disease (Athari et al 2007;Decarneri and Giannone 1964;Dudko and Kurnatowska 2007;Linke et al 1989;Marty et al 2015;Ribeiro et al 2015). In this study, trichomonads were found to be widely distributed in canine dental plaque.…”

Section: %supporting

confidence: 46%

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

Patel

Colyer²,

Harris³

et al. 2016

J Eukaryotic Microbiology

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Periodontal disease is one of the most important health concerns for companion animals. Research into canine forms of periodontitis has focused on the identification and characterization of the bacterial communities present. However, other microorganisms are known to inhabit the oral cavity and could also influence the disease process. A novel, broad spectrum 18S PCR was developed and used, in conjunction with next-generation sequencing analyses to target the identification of protists. Trichomonas sp. and Entamoeba sp. were identified from 92 samples of canine plaque. The overall prevalence of trichomonads was 56.52% (52/92) and entamoebae was 4.34% (4/92). Next-generation sequencing of pooled healthy, gingivitis, earlystage periodontitis, and severe periodontitis samples revealed the proportion of trichomonad sequences to be 3.51% (health), 2.84% (gingivitis), 6.07% (early periodontitis), and 35.04% (severe periodontitis), respectively, and entamoebae to be 0.01% (health), 0.01% (gingivitis), 0.80% (early-stage periodontitis), and 7.91% (severe periodontitis) respectively. Both genera of protists were statistically associated with plaque from dogs with periodontal disease. These findings provide the first conclusive evidence for the presence of oral protozoa in dog plaque and suggest a possible role for protozoa in the periodontal disease process.

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Section: %supporting

confidence: 46%

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

Patel

Colyer²,

Harris³

et al. 2016

J Eukaryotic Microbiology

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Section: Discussionmentioning

confidence: 99%

“…Dental practitioners should take time to analyse the intra-sulcular dental plaque of patients with periodontitis by direct microscopy. The discovery of T. tenax may be a marker of a specific clinical type of periodontitis and modify the therapeutic management and prognosis (Marty et al 2015). F I N A N C I A L S U P P O R T This study was supported by funding from the Midi-Pyrenees region, France, Paul Sabatier University and the research platform of Toulouse Dental Faculty (PLTRO).…”

Section: Discussionmentioning

confidence: 99%

“…Giemsa or trichrome staining) or culturing to identify T. tenax in dental plaque samples. Only four studies used the polymerase chain reaction (PCR) assay to highlight this pathogen (Athari et al 2007;Kurnatowska et al 2004a;Turkowicz et al 2004;Mehr et al 2015).…”

Section: R E S U L T Smentioning

confidence: 99%

“…Nevertheless, such a method is used at chair-side for diagnosis and treatment management ( Fig. 1) but it could give rise to more false negative results (Marty et al 2015). In vitro culture is also a method for detecting the presence of the protozoa (Table 1), but its ability to detect T. tenax was found to be similar to that of direct observation (El Sibaei et al 2012).…”

Section: Main Objectivementioning

confidence: 99%

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Trichomonas tenax and periodontal diseases: a concise review

et al. 2017

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Periodontal diseases (gingivitis and periodontitis), result from a disruption of the host-oral microbiome homoeostasis. Whereas the pathological role of some specific bacterial strains during periodontal diseases is well documented, the impact of parasites in periodontium pathophysiology is still under debate. This review aims to collect data about the prevalence and the potential role of Trichomonas tenax during periodontal diseases. Data from 47 studies revealed that T. tenax prevalence in diseased periodontium ranged from 0 to 94·1%. The prevalence of oral protozoan infections was found to be largely greater in patients with periodontal diseases than with healthy periodontium. The parasite detection was mainly performed by direct microscopy. Trichomonas tenax presence was clearly correlated with periodontal disease. The high heterogeneity of its periodontal prevalence may be correlated with the diversity of the population screened (age, sex, systemic diseases), and the methods used for diagnosis. This protozoan seems to have the capacity to be involved in the inflammatory process of gum disease. Animal experimentation, using relevant physiopathological models of periodontitis, needs to be performed to investigate the ability of T. tenax to cause and/or worsen the disease. Further investigations using standardized experimental designs of epidemiologic studies are also needed.

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Direct Microscopy: A Useful Tool to Diagnose Oral Candidiasis in Children and Adolescents

et al. 2015

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Observation of trichomonads infection in a child with periodontitis by direct microscopy at the dental office

Cited by 5 publications

References 11 publications

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

Trichomonas tenax and periodontal diseases: a concise review

Direct Microscopy: A Useful Tool to Diagnose Oral Candidiasis in Children and Adolescents

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