Observation of the selective staining of chromosomal DNA in dividing cells using a luminescent terbium(iii) complex

Law, Ga Lai; Man, Cornelia; Parker, David; Walton, James W.

doi:10.1039/b924679g

Cited by 55 publications

(36 citation statements)

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“…Barton and Gilles also found that Ru(II) polypyridyl complexes not only exerted biological effects but also could be the luminescence cellular imaging probes, specially for RNA mismatches [18,28e30]. Meanwhile, Che et al found that Au(III) complexes containing N-heterocyclic carbene ligands could be the thiol "switch-on" fluorescent probes and anti-cancer agents [31], while Parker et al observed the selective staining of chromosomal DNA in dividing cells using a luminescent Tb(III) complex [32]. Importantly, Vicente et al found that the intracellular localization of the Zn(II)-phthalocyanines determined their photodynamic activity [33].…”

Section: Introductionmentioning

confidence: 98%

Cellular localization of iron(II) polypyridyl complexes determines their anticancer action mechanisms

et al. 2015

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Section: Introductionmentioning

confidence: 98%

Cellular localization of iron(II) polypyridyl complexes determines their anticancer action mechanisms

et al. 2015

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“…was synthesised according to previously described methods. 137 Complexation was confirmed by mass spectrometry prior to working up the reaction. The complex was then purified by preparative HPLC and isolated as an off-white solid.…”

Section: Studies Using Circular Dichroismmentioning

confidence: 99%

“…This was an unexpected result, as the q' value given in the literature for the racemic parent complex [Ln.L 22 ] + was ~ 0 for both the europium and terbium analogues. 137 The reproducibility of this literature result was subsequently investigated; using the methodology outlined for the complexes of L…”

Section: Estimation Of the Complex Hydration State Q'mentioning

confidence: 99%

Lanthanide complexes as chiral probes exploiting circularly polarized luminescence

2012

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The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source• a link is made to the metadata record in Durham E-Theses• the full-text is not changed in any wayThe full-text must not be sold in any format or medium without the formal permission of the copyright holders. Statement of CopyrightThe copyright of this thesis rests with the author. No quotations should be published without prior consent and information derived from it must be acknowledged. ABSTRACT -ii - ABSTRACTA series of studies has been undertaken to facilitate the identification and development of chiral lanthanide complexes that are able to report on changes in their local environment through modulation of the circular polarization of their emission. Reports of such systems remain relatively rare in the literature, notwithstanding the prevalence and importance of chirality in biological systems.The work described herein is separated into five chapters, the first of which comprises a discussion of relevant background information, along with a comprehensive review of responsive lanthanide-based CPL probes reported to date. A classification of these probes is built up, which informs the content of the following three chapters.Chapter 2 describes work undertaken in the pursuit of a novel lanthanide-based system for use as a CPL probe for the detection of proteins. The synthesis of an enantiopure lanthanide complex was undertaken and characterisation of this system carried out with reference to a structurally related racemic complex. A series of comparative investigations designed to probe the relative protein binding capability of these complexes was subsequently performed, which revealed that the observation of induced CPL from racemic lanthanide systems may be brought about by a change in complex constitution. This is the first example of such an effect from a well-defined racemic lanthanide complex in solution. Chapter 3 goes on to detail studies undertaken to demonstrate the utility of this racemic lanthanide system as a probe for chiral detection.Chapter 4 describes investigations carried out in an attempt to identify new systems exhibiting chiral quenching effects in solution. Initially, two pairs of enantiomeric electron-rich quenching species were assessed for their ability to quench the emission from an enantiopure DOTA-derived lanthanide complex differentially. Subsequently, investigations were focussed on examining the quenching of emission from novel enantiopure lanthanide complexes based on a 1,4,7-triazacyclononane framework, using cobalt complexes as the quenching species.Finally, Chapter 5 contains experimental procedures for each compound synthesised, as well as general experimental procedures.

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“…Based on this intercalative mechanism, many cationic planar, polycyclic dyes derived from quinolizinium, benzooxazolinium, benzothiazolinium, and acridizinium have been developed as powerful optical probes for DNA detection [9,10]. These DNA-selective probes demonstrate a variety of potential applications in pharmaceutical studies and molecular biology, and can be used as probes for electron and/or energy transfer and gene modulators [11][12][13][14][15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

A Novel Styryldehydropyridocolinium Homodimer: Synthesis and Fluorescence Properties Upon Interaction with DNA

et al. 2015

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A novel homodimer of the styryldehydropyridocolinium dye (TPTP) has been synthesized and characterized. Free TPTP exhibited low fluorescence quantum yield and large Stokes shift (over 160 nm) in water. However, it showed a significant fluorescence turn-on effect upon intercalation into DNA base pairs. Meanwhile, the fluorescence intensity of the intercalated structures formed by TPTP and DNA decreased quickly upon addition of deoxyribonuclease I, indicating that the dye can be used to monitor deoxyribonuclease I activity and DNA hydrolysis. Electrophoresis analysis revealed that the dye had intercalative binding to DNA and can potentially be used for DNA staining in electrophoresis. Thus, the innate nature of large Stokes shift and excellent fluorescence turn on effect upon interaction with DNA endue the dye with a wide range of applications.

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Observation of the selective staining of chromosomal DNA in dividing cells using a luminescent terbium(iii) complex

Abstract: The emission intensity of a monocationic Tb or Eu(III) complex of a bis(1-azaxanthone) ligand is enhanced in the presence of serum albumin; the lanthanide complex stains dividing cells, allowing visualisation of mitotic chromosomes.

Cited by 55 publications

References 27 publications

Cellular localization of iron(II) polypyridyl complexes determines their anticancer action mechanisms

Cellular localization of iron(II) polypyridyl complexes determines their anticancer action mechanisms

Lanthanide complexes as chiral probes exploiting circularly polarized luminescence

A Novel Styryldehydropyridocolinium Homodimer: Synthesis and Fluorescence Properties Upon Interaction with DNA

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