Observation of Red Blood Cell Membrane Charge Using Magnetic Beads Under Pulsed Magnetic Field

Choi, Yukyoung; Bang, Seunghwan; Lee, Hyun Sook

doi:10.1109/tmag.2021.3129887

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Biomedical phage use will be assisted by a more efficient (in time and cost) way to rapidly identify and screen out low-persistence phages. We suspect that useful "screening-out" criteria include either a positive σ or a negative σ relatively low in magnitude, as found for the agglutination of red blood cells [38][39][40].…”

Section: Native Agarose Gel Electrophoresis and Murine Persistencementioning

confidence: 97%

Siphophage 0105phi7-2 of Bacillus thuringiensis: Novel Propagation, DNA, and Genome-Implied Assembly

Roberts,

Aldis,

Wright

et al. 2023

IJMS

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Diversity of phage propagation, physical properties, and assembly promotes the use of phages in ecological studies and biomedicine. However, observed phage diversity is incomplete. Bacillus thuringiensis siphophage, 0105phi-7-2, first described here, significantly expands known phage diversity, as seen via in-plaque propagation, electron microscopy, whole genome sequencing/annotation, protein mass spectrometry, and native gel electrophoresis (AGE). Average plaque diameter vs. plaque-supporting agarose gel concentration plots reveal unusually steep conversion to large plaques as agarose concentration decreases below 0.2%. These large plaques sometimes have small satellites and are made larger by orthovanadate, an ATPase inhibitor. Phage head–host-cell binding is observed by electron microscopy. We hypothesize that this binding causes plaque size-increase via biofilm evolved, ATP stimulated ride-hitching on motile host cells by temporarily inactive phages. Phage 0105phi7-2 does not propagate in liquid culture. Genomic sequencing/annotation reveals history as temperate phage and distant similarity, in a virion-assembly gene cluster, to prototypical siphophage SPP1 of Bacillus subtilis. Phage 0105phi7-2 is distinct in (1) absence of head-assembly scaffolding via either separate protein or classically sized, head protein-embedded peptide, (2) producing partially condensed, head-expelled DNA, and (3) having a surface relatively poor in AGE-detected net negative charges, which is possibly correlated with observed low murine blood persistence.

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Section: Native Agarose Gel Electrophoresis and Murine Persistencementioning

confidence: 97%

Siphophage 0105phi7-2 of Bacillus thuringiensis: Novel Propagation, DNA, and Genome-Implied Assembly

Roberts,

Aldis,

Wright

et al. 2023

IJMS

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“…The more oxygen available, the more ATP may be produced and available for energy expenditure [19]. The energy pulse generated by the cm 2 PEMF device appears to affect erythrocyte zeta potential [20], improving separation of the users' erythrocytes and, consequently, improving oxygen delivery and increased ATP production.…”

Section: Introductionmentioning

confidence: 99%

Impact of Novel Pulsed Electromagnetic Field Device on Competitive Athlete Performance

Gledhill,

Huntsman,

Anderson

2023

OJBIPHY

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The interaction of pulsed electromagnetic fields (PEMF) with the human body may result in a variety of positive outcomes including analgesia, enhanced healing, chondroprotection, cognitive improvement and better quality of life. Previous human studies have also revealed the potential of PEMF to enhance muscle function and athletic performance. To further evaluate this potential, an open label pilot study was conducted with 19 competitive cyclists who repeatedly participated in 63 training routes. Cyclist performance was tracked before and during use of a novel and portable PEMF device that is worn as a wristband. Comparison of performance before and during use of the wristband revealed a significant association with improved muscle power. The odds ratio was 3.02 (P < 0.01) for experiencing increased muscle power while wearing the PEMF device. Among the cycling routes in which an increase was observed, the average increase in power was about 9.8%. The data suggests the novel PEMF technology may be a safe and effective therapeutic approach for improved physical performance and likely involves improved oxygen delivery due to reduced rouleaux (erythrocyte aggregation). These results warrant further investigation comprising larger studies and additional outcomes.

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Effect of pulsed magnetic field in murine T lymphoma EL4 cells

Lee

Kim

et al. 2023

AIP Advances

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Maintenance of homoeostasis in human body is a very important indicator in all cell activities. When exposed to a disease, various immune cells are activated due to the inflammatory response, and particularly T cells play a role in inducing apoptosis of mutated cells such as tumor cells. When the activity of T cells is very low, infection by external invasion is easy, and on the contrary, excessive activation leads to chronic inflammation caused by autoimmune diseases. Many clinical studies related to pulsed magnetic field (PMF) demonstrated its efficacy in reducing pain, improving blood circulation, as well as blood’s acid-base balance. Therefore, our study has tried to investigate the influence of PMF on the regulation of acid-base homeostasis in EL4 T lymphoma cell. In addition, we have tried to explain the role of PMF on immune cell activity by measuring the level of pro-inflammatory cytokine, TNF-α in culture supernatants. EL4 cells were cultured in a DMEM medium supplemented with 10% FBS and 1% penicillin in an incubator at 37 °C and 5% CO2 condition. Our PMF stimulator has the maximum strength of 4700 G at a transition time of 222 μs with pulse intervals of 1 Hz. The homoeostasis in pH was improved as PMF strength increases. Cell viability decreased by 32% after PMF stimulation of 4700 G. It was observed that the concentration of TNF-α, a cytokine related to inflammation, also decreased as the strength of PMF increased. These results suggest that PMF stimulation improves the anti-inflammatory effect, therefore, it is thought to affect the immune system by balancing the activation and suppression of immune cells. For clinical use, our study might suggest non-invasive PMF can be developed as a medical devices modulating immune system, although it is necessary to optimize the PMF conditions such as pulse shape, duration, or repetition rate.

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Observation of Red Blood Cell Membrane Charge Using Magnetic Beads Under Pulsed Magnetic Field

Cited by 4 publications

References 13 publications

Siphophage 0105phi7-2 of Bacillus thuringiensis: Novel Propagation, DNA, and Genome-Implied Assembly

Siphophage 0105phi7-2 of Bacillus thuringiensis: Novel Propagation, DNA, and Genome-Implied Assembly

Impact of Novel Pulsed Electromagnetic Field Device on Competitive Athlete Performance

Effect of pulsed magnetic field in murine T lymphoma EL4 cells

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