Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics

Kotobuki, Noriko; Ioku, Koji; Kawagoe, Daisuke; Fujimori, Hirotaka; Goto, Seishi; Ohgushi, Hajime

doi:10.1016/j.biomaterials.2004.03.020

Cited by 137 publications

(101 citation statements)

References 15 publications

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“…Various in vitro bone regeneration strategies have relied on the use of the osteogenic supplements (dexamethasone, ascorbic acid, and b-glycerophosphate) to induce mineralization in vitro, 12,13,49,51,[53][54][55][56][57][58][59][60][61][62] and each supplement has been shown to be imperative to induce mineralization of MSCs in vitro. 9,[11][12][13][14][15][16][17]22,29,[63][64][65][66][67] The main rationale behind the use of these supplements is to provide signaling proteins or enzymes to activate mineral production by MSCs.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic Differentiation of Mesenchymal Stem Cells by Mimicking the Cellular Niche of the Endochondral Template

Freeman

Stevens

Owens

et al. 2016

Tissue Engineering Part A

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In vitro bone regeneration strategies that prime mesenchymal stem cells (MSCs) with chondrogenic factors, to mimic aspects of the endochondral ossification process, have been shown to promote mineralization and vascularization by MSCs both in vitro and when implanted in vivo. However, these approaches required the use of osteogenic supplements, namely dexamethasone, ascorbic acid, and b-glycerophosphate, none of which are endogenous mediators of bone formation in vivo. Rather MSCs, endothelial progenitor cells, and chondrocytes all reside in proximity within the cartilage template and might paracrineally regulate osteogenic differentiation. Thus, this study tests the hypothesis that an in vitro bone regeneration approach that mimics the cellular niche existing during endochondral ossification, through coculture of MSCs, endothelial cells, and chondrocytes, will obviate the need for extraneous osteogenic supplements and provide an alternative strategy to elicit osteogenic differentiation of MSCs and mineral production. The specific objectives of this study were to (1) mimic the cellular niche existing during endochondral ossification and (2) investigate whether osteogenic differentiation could be induced without the use of any external growth factors. To test the hypothesis, we evaluated the mineralization and vessel formation potential of (a) a novel methodology involving both chondrogenic priming and the coculture of human umbilical vein endothelial cells (HUVECs) and MSCs compared with (b) chondrogenic priming of MSCs alone, (c) addition of HUVECs to chondrogenically primed MSC aggregates, (d-f) the same experimental groups cultured in the presence of osteogenic supplements and (g) a noncoculture group cultured in the presence of osteogenic growth factors alone. Biochemical (DNA, alkaline phosphatase [ALP], calcium, CD31 + , vascular endothelial growth factor [VEGF]), histological (alcian blue, alizarin red), and immunohistological (CD31 + ) analyses were conducted to investigate osteogenic differentiation and vascularization at various time points (1, 2, and 3 weeks). The coculture methodology enhanced both osteogenesis and vasculogenesis compared with osteogenic differentiation alone, whereas osteogenic supplements inhibited the osteogenesis and vascularization (ALP, calcium, and VEGF) induced through coculture alone. Taken together, these results suggest that chondrogenic and vascular priming can obviate the need for osteogenic supplements to induce osteogenesis of human MSCs in vitro, while allowing for the formation of rudimentary vessels.

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Section: Discussionmentioning

confidence: 99%

Osteogenic Differentiation of Mesenchymal Stem Cells by Mimicking the Cellular Niche of the Endochondral Template

Freeman

Stevens

Owens

et al. 2016

Tissue Engineering Part A

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“…Although the histological analysis of the scaffolds partially overcomes this issue, the development of transparent hydroxyapatites could be very useful for investigations in which fluorescence analyses are performed. Two-dimensional films of transparent hydroxyapatite (tHA) that were made by the spark plasma sintering process have been developed and used as substrate to culture rat MSCs up to 14 days (Kotobuki et al, 2005). Attachment, proliferation and osteogenic differentiation observed by light microscopy were similar to those obtained on tissue culture polystyrene dishes.…”

Section: Culturing Osteogenic Cells In Bioceramic Based Scaffoldsmentioning

confidence: 70%

Three-dimensional cultures of osteogenic and chondrogenic cells: A tissue engineering approach to mimic bone and cartilage in vitro

Tortelli

Cancedda²

2009

eCM

103

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Capturing the complexity of bone and cartilage into three-dimensional in vitro models remains one of the most important challenges in the field of the tissue engineering. Indeed, the development and the optimization of novel culture systems may be necessary to face the next questions of bone and cartilage physiology. The models should faithfully mimic these tissues, resembling their organization, their mechanical properties and their physiological response to different stimuli. Here we review the recent advances in the field of the three-dimensional cultures of both osteogenic and chondrogenic cells. In particular, we highlight the most important studies that, to our knowledge, have investigated the response of the cells to the three-dimensional environment provided by the diverse types of scaffold.

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“…More to the point, investigation of rat calvaria osteoblasts cultured on transparent HA bioceramics, as well as the analysis of osteogenic-induced human bone marrow stromal cells at different time points of culturing indicated to a good http://ccaasmag.org/BIO cytocompatibility of HA bioceramics and revealed favorable cell proliferation [368]. Positive results for other types of cells have been obtained in other studies [206,363,364,367].…”

Section: Bioactivitymentioning

confidence: 77%

“…Thus, transparent calcium orthophosphate bioceramics (Fig. 5) [361] have been prepared and investigated [204,206,289,292,[361][362][363][364][365][366][367][368][369][370]. They can exhibit an optical transmittance of ~ 66 % at a wavelength of 645 nm [370].…”

Section: Possible Transparencymentioning

confidence: 99%

Untitled

2011

BIO

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In the late 1960's, a strong interest was raised in biomedical applications of ceramic materials (named bioceramics a little bit later). Bioceramics was initially used as reasonable alternatives to metals in order to increase their biocompatibility. However, within a short period, it has grown into a diverse class of biomaterials, presently including three essential types: relatively bioinert, bioactive (or surface reactive) and bioresorbable bioceramics. This review is limited to bioceramics prepared from calcium orthophosphates only, which belong to the categories of bioactive and bioresorbable compounds. There have been a number of important advances in this field during the past 30 -40 years. The research was shifted from an initial study on the develop-ment of bioinert bioceramics towards bioactive and bioresorbable bioceramics, and these different types of calcium orthophosphate bioceramics can be tuned through the structural and compositional control. At the turn of the millennium, a new concept of calcium orthophosphate bioceramics has been developed to promote a regeneration of bones. Current biomedical applications of calcium orthophosphate bioceramics include artificial replacements for hips, knees, teeth, tendons and ligaments, as well as repair for periodontal disease, maxillofacial reconstruction, augmenttation and stabilization of the jawbone, spinal fusion and bone fillers after tumor surgery. Potential future applications of calcium orthophosphate bioceramics are demonstrated in drug delivery systems, effective carriers of growth factors, bioactive peptides and/or various types of cells for tissue engineering purposes.

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Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics

Cited by 137 publications

References 15 publications

Osteogenic Differentiation of Mesenchymal Stem Cells by Mimicking the Cellular Niche of the Endochondral Template

Osteogenic Differentiation of Mesenchymal Stem Cells by Mimicking the Cellular Niche of the Endochondral Template

Three-dimensional cultures of osteogenic and chondrogenic cells: A tissue engineering approach to mimic bone and cartilage in vitro

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