Observation of nanosecond laser induced fluorescence of in vitro seawater phytoplankton

Bensky, Thomas J.; Clemo, L.; Gilbert, Chris; Neff, B.; Moline, Mark A.; Rohan, Dov

doi:10.1364/ao.47.003980

Cited by 13 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescent lifetime of chlorophyll can vary between 0.3 [6] and 10 ns [7] depending on various factors such as temperature, concentration and the overall condition of the Chlorophyll sample. A realistic minimum target of 1.0-2.0 ns is taken as it correlates well with the fast fluorescent lifetimes typical with chlorophyll-A in seawater samples described in [13]. In typical phase fluorometers, optimal noise immunity is obtained for excitation frequencies that are related to the fluorescent lifetime under study by the relationship (8) which, for this application, gives an optimal frequency of 79.6 MHz.…”

Section: A Custom Tia Integrated Circuitmentioning

confidence: 99%

Portable Fluorescence Lifetime Detection for Chlorophyll Analysis in Marine Environments

Kissinger

Wilson

2011

IEEE Sensors J.

View full text Add to dashboard Cite

This paper describes the proof-of-concept performance of a low-cost phase fluorometer designed to capture the fluorescence lifetime of chlorophyll in various stages of healthy marine life. The proof-of-concept experimental demonstration is completed using fluoroscein as a close simulant of chlorophyll. Results are extrapolated analytically using simulation to project performance limits (detection and lifetime) in chlorophyll rich environments. The designed system is able to compete with the fundamental performance limits of existing fluorometers designed for chlorophyll analysis while reducing power consumption by a factor of 20, power supply by an order of magnitude or more (to 12 V), and cost by a factor of ten (to a target low-volume system cost of $1000). Index Terms-Biologicalsensor, chemical sensor, fluorescence, intelligent sensors, phase fluorometry. Jeffrey Kissinger received the M.S. degree in electrical engineering from the University of Washington, Seattle, in 2009. He is currently an Electrical Engineer with BE Meyers, Redmond, WA, and has past experience working with National Semiconductor, Tucson, AZ. His research and technical interests focus on analog circuit design. Denise Wilson (M'96) received the B.S. degree in mechanical engineering from Stanford University, Stanford, CA, in 1988, and the M.S. and Ph.D. degrees in electrical engineering from the Georgia Institute of Technology, Atlanta, in 1989 and 1995, respectively. She is currently an Associate Professor with the Department of Electrical Engineering, University of Washington, Seattle. Her research interests focus on the development of signal processing architectures, array platforms, and other infrastructures for visual, auditory, and chemical-sensing platforms.

show abstract

Section: A Custom Tia Integrated Circuitmentioning

confidence: 99%

Portable Fluorescence Lifetime Detection for Chlorophyll Analysis in Marine Environments

Kissinger

Wilson

2011

IEEE Sensors J.

View full text Add to dashboard Cite

show abstract

“…A modified bioluminescence sensor described by Herren et al (2005), for example, can now sample at 60 Hz, which would improve the horizontal resolution by at least an order of magnitude. An ultra-high sampling frequency chlorophyll a fluorometer is also under development for the AUV (Bensky et al, 2008), which would have a significant effect on resolving the micro-scale. Although technically challenging, the installation of calibrated multi-frequency echosounders on AUVs, has the potential to improve the measurement of the horizontal extent of thin layers.…”

Section: Sampling and Spatial Scales Of Layersmentioning

confidence: 99%

Integrated measurements of acoustical and optical thin layers II: Horizontal length scales

Moline

Benoit‐Bird

Robbins

et al. 2010

Continental Shelf Research

Self Cite

View full text Add to dashboard Cite

a b s t r a c tThe degree of layered organization of planktonic organisms in coastal systems impacts trophic interactions, the vertical availability of nutrients, and many biological rate processes. While there is reasonable characterization of the vertical structure of these phenomena, the extent and horizontal length scale of variation has rarely been addressed. Here we extend the examination of the vertical scale in the first paper of the series to the horizontal scale with combined shipboard acoustic measurements and bio-optic measurements taken on an autonomous underwater vehicle. Measurements were made in Monterey Bay, CA from 2002 to 2008 for the bio-optical parameters and during 2006 for acoustic scattering measurements. The combined data set was used to evaluate the horizontal decorrelation length scales of the bio-optical and acoustic scattering layers themselves. Because biological layers are often decoupled from the physical structure of the water column, assessment of the variance within identified layers was appropriate. This differs from other studies in that physical parameters were not used as a basis for the layer definition. There was a significant diel pattern to the decorrelation length scale for acoustic layers with the more abundant nighttime layers showing less horizontal variability despite their smaller horizontal extent. A significant decrease in the decorrelation length scale was found in bio-optical parameters over six years of study, coinciding with a documented shift in the plankton community. Results highlight the importance of considering plankton behavior and time of day with respect to scale when studying layers, and the challenges of sampling these phenomena.

show abstract

“…yellow substance and pollutants. Bensky et al [17] observed instantaneous fluorescent emission from Chlorophyll in phyto-plankton resident in vitro seawater samples as a result of pumping with a 440 nm, 70 ns laser pulse.…”

Section: Introductionmentioning

confidence: 99%

Feasibility study for airborne fluorescence/reflectivity lidar bathymetry

et al. 2012

View full text Add to dashboard Cite

There is a demand from the authorities to have good maps of the coastal environment for their exploitation and preservation of the coastal areas. The goal for environmental mapping and monitoring is to differentiate between vegetation and non-vegetated bottoms and, if possible, to differentiate between species. Airborne lidar bathymetry is an interesting method for mapping shallow underwater habitats. In general, the maximum depth range for airborne laser exceeds the possible depth range for passive sensors. Today, operational lidar systems are able to capture the bottom (or vegetation) topography as well as estimations of the bottom reflectivity using e.g. reflected bottom pulse power. In this paper we study the possibilities and advantages for environmental mapping, if laser sensing would be further developed from single wavelength depth sounding systems to include multiple emission wavelengths and fluorescence receiver channels. Our results show that an airborne fluorescence lidar has several interesting features which might be useful in mapping underwater habitats. An example is the laser induced fluorescence giving rise to the emission spectrum which could be used for classification together with the elastic lidar signal. In the first part of our study, vegetation and substrate samples were collected and their spectral reflectance and fluorescence were subsequently measured in laboratory. A laser wavelength of 532 nm was used for excitation of the samples. The choice of 532 nm as excitation wavelength is motivated by the fact that this wavelength is commonly used in bathymetric laser scanners and that the excitation wavelengths are limited to the visual region as e.g. ultraviolet radiation is highly attenuated in water. The second part of our work consisted of theoretical performance calculations for a potential real system, and comparison of separability between species and substrate signatures using selected wavelength regions for fluorescence sensing.

show abstract

Observation of nanosecond laser induced fluorescence of in vitro seawater phytoplankton

Cited by 13 publications

References 24 publications

Portable Fluorescence Lifetime Detection for Chlorophyll Analysis in Marine Environments

Portable Fluorescence Lifetime Detection for Chlorophyll Analysis in Marine Environments

Integrated measurements of acoustical and optical thin layers II: Horizontal length scales

Feasibility study for airborne fluorescence/reflectivity lidar bathymetry

Contact Info

Product

Resources

About