Observation of inflammation-induced mitophagy during stroke by a mitochondria-targeting two-photon ratiometric probe

Abstract

This study reports the development of a new, pH-sensitive, mitochondria-targeting two-photon ratiometric probe (Mito-BNO) for real-time tracking of mitophagy, a process that accelerates in brain tissue during stroke. Mito-BNO demonstrated...

Mitochondrial Quality Control: A Pathophysiological Mechanism and Therapeutic Target for Stroke

“…It subsequently enhanced the translocation of the mitochondria to lysosomes for autophagy. Through a mitochondria-targeting two-photon ratiometric probe, enhanced mitophagy was demonstrated at 0, 3, 6, and 12 h after ODG/R in PC12 cells (Cheng F. et al, 2021). Increased mitophagy may be related to oxidative stress.…”

“…Increased mitophagy may be related to oxidative stress. Antiinflammatory treatment can partially inhibit mitophagy (Cheng F. et al, 2021). Under physiological conditions, the autophagy adaptors BNIP3 and Beclin1 bind with Bcl-2 at the BH3 domain, which decreases mitophagy activity.…”

Simultaneous tracking of autophagy and oxidative stress during stroke with an ICT-TBET integrated ratiometric two-photon platform

“…11,12 In particular, two-photon confocal technology using focused excitation can signicantly improve the imaging resolution and visualization time. 13,14 Single emission probes are affected by many factors, such as light source intensity, solvent scattering, color and viscosity, probe distribution, instrumental performance and so on, which results in inaccuracy, especially in the determination of the starting point of metabolism. 15 As such there is a need to develop improved multi emission probes (ratiometric probes) with reduced crosstalk.…”

Observation of macrophage autophagy in the healing of diabetic ulcers via a lysosome-targeting polarity-specific two-photon probe

“… 12 Secondly, its near-infrared excitation has less damage to the biological samples, making it a suitable method for long-term imaging and observation of the biological samples. 13 Thirdly, photobleaching outside the focus is avoided due to its focus excitation property, which improves the spatial resolution. Finally, the deep penetration of near-infrared excitation makes it a good tool for deep tissue imaging.…”