Observation of Growth of Human Fibroblasts on Silver Nanoparticles

Abstract

Silver nanoparticles have drawn extensive attention as biomaterial components. Human fibroblasts were grown on various concentrations of silver nanoparticles during the period observation. Normal viability (0% silver particles) was increased from 6 to 72 hours, increasing the amount of human fibroblasts (1.5x10 4 to 7x10 6 cells/well) normally. Nevertheless, at higher concentrations of silver nanoparticles (50%) 1.11x10 5 cells/well remained after 72 hours. Results indicated that the increase in the concentration of silver nanoparticles reduced the number of fibroblasts and affected their fission. Silver nanoparticles were found under the membranes of fibroblast following dry treatment. The number of tissues declined because the silver nanoparticles interrupted the fission mechanism during their development in vivo.

A review on the application of inorganic nano-structured materials in the modification of textiles: Focus on anti-microbial properties

“…Similar mechanism has been quoted for silver ions and nano particles [125]. Silver nano-particles are a non-toxic and non-tolerant disinfectant [128][129][130]. Using silver nano-particles leads to increase in number of particles per unit area and, thus, anti-bacterial effects can be maximized [131].…”

“…Some examples can be pointed out as follows: Skin-innoxiousness of nano-silver colloidal solution especially in the case of smaller nano-particles has been demonstrated via the skin irritation test performed on the rabbits [140]. Nontoxicity of the interaction of nano-silver and the membrane surface has been proved by growing human fibroblasts on various concentrations of silver nano-particles [128]. Silver is mentioned as an almost non-toxic to mammalian systems [317] Incubation of astrocyte primary cultures with magnetic iron oxide nano-particles has also demonstrated that the particles do not induce any acute damage to these brain cells [316].…”

Antibacterial Potential of Jatropha curcas Synthesized Silver Nanoparticles against Food Borne Pathogens

“…Although the actual mechanism of AgNps functioning is not yet clear, researchers believe that AgNps interact with thiol group of bacterial proteins (Lok et al, 2006) and phosphorous moieties of DNA (Rai et al, 2009) to inactivate the bacterial cell system. It was also reported that nano-silver is non-toxic to humans at low doses (Wen et al, 2007; Tankhiwale and Bajpai, 2009). Thereafter, few attempts were made to develop AgNps coated systems, especially for food preservation against lethal food borne pathogens.…”

Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity

“…36 Interaction of AgNPs with human fibroblasts affected their fission, but it was not toxic. 37 Though AgNPs are reported to cause no big health problems when administered into the body, argyria is always a problem, irrespective of the nanosize of silver. 38 In daily life, AgNPs-impregnated clothing may find a good applicability in underwear and socks.…”