Observation of chondrocyte aggregate formation and internal structure on micropatterned fibroin-coated surface

Kachi, Naoyoshi; Otaka, Akira; Sim, Seungwoo; Kuwana, Yoshihiko; Tamada, Yasushi; Sunaga, Junko; Adachi, Taiji; Tomita, Naohide

doi:10.3233/bme-2010-0615

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…In vitro cultures porous silk scaffolds showed the best results; the GAG (glycosaminoglycan) content was also higher. Based on previous experiences on the ability of silk fibroin scaffolds to behave in the same way as ECM (extracellular matrix) towards chondrocytes [103], some researchers have fabricated curcumin and silk scaffolds with saline leaching method [104]. The scaffolds obtained were porous and rough, thus favoring cell adhesion [105].…”

Section: E) Cartilagementioning

confidence: 99%

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

Bibbò¹,

Khan²,

Tareen³

2021

GJRE

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Silk, seen as a material, is a fiber made from silkworm cocoons and spiders. They have standard structural components and hierarchical structures. Different manufacturing techniques allow obtaining silk in films, fibers, hydrogels, microspheres, and sponges. We can tune the properties through the structure of secondary proteins. The paper explores the application in biomedical, optics, and electronic fields by analyzing the technological trend.

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Section: E) Cartilagementioning

confidence: 99%

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

Bibbò¹,

Khan²,

Tareen³

2021

GJRE

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“…Cell migration is one of the most important processes involved in the regeneration of damaged tissue [35,36], and tissue-specific chemo-attractant treatment can induce the migration of relevant cells that promote tissue formation at defect sites [37]. There are several reports indicating that uncrosslinked/degraded ECM molecules entrapped in hydrogels may be released over time to form a gradient, and direct migration of cells near ECM hydrogels [30][31][32]38].…”

Section: Migration Of Cells Near Ecm Hydrogelsmentioning

confidence: 99%

A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting

et al. 2014

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Bioprinting is a promising technique for engineering composite tissues, such as osteochondral tissues. In this study, as a first step toward bioprinting-based osteochondral tissue regeneration, we systematically examined the behavior of chondrocytes and osteoblasts to hyaluronic acid (HA) and type I collagen (Col-1) hydrogels. First, we demonstrated that cells on hydrogels that were comprised of major native tissue extracellular matrix (ECM) components (i.e. chondrocytes on HA hydrogels and osteoblasts on Col-1 hydrogels) exhibited better proliferation and cell function than cells on non-native ECM hydrogels (i.e., chondrocytes on Col-1 hydrogels and osteoblasts on HA hydrogels). In addition, cells located near their native ECM hydrogels migrated towards them. Finally, we bioprinted three-dimensional (3D) osteochondral tissue-mimetic structures composed of two compartments, osteoblast-encapsulated Col-1 hydrogels and chondrocyte-encapsulated HA hydrogels, and found viability and functions of each cell type were well maintained within the 3D structures up to 14 days in vitro. These results suggest that with proper choice of hydrogel materials, bioprinting-based approaches can be successfully applied for osteochondral tissue regeneration.

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A Hybrid Model of Cartilage Regeneration Capturing the Interactions Between Cellular Dynamics and Porosity

Cassani

Olson

2020

Bull Math Biol

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To accelerate the development of strategies for cartilage tissue engineering, models are necessary to investigate the interactions between cellular dynamics and scaffold porosity. In experiments, cells are seeded in a porous scaffold or hydrogel where over the time course of a month, the scaffold slowly degrades while cells divide and synthesize extracellular matrix constituents. We use an off-lattice cellular automaton framework to model the individual behavior of cells within the scaffold. The movement of cells and the ability to reproduce is determined by the nutrient profile in the construct and/or the local porosity, defined as the volume fraction of fluid in the surrounding region. A phenomenological approach is used to capture a continuous profile for the degrading scaffold and accumulating matrix, which will then change the local porosity throughout the construct. We parameterize the model by first matching total cell counts in the construct to chondrocytes seeded in a polyglycolic acid scaffold (Freed et al., 1994). We investigate the total cell count and location of different cell populations within the construct for different initial scaffold porosities. Similar to experiments, we observe cell counts that level off around day 15 with higher cell counts in scaffolds of higher initial solid volume fraction (lower porosity). Cell clustering is observed and characterized in regions at the edge of the construct that are close to the nutrient-rich medium in the fluid bath. Model results show that a bias in motion due to a sensitivity to porosity allows cells to move in a smarter or more optimal arrangement. We investigate the spatiotemporal distribution of cells as the cell reproduction rate, cell movement distance, and sensitivity to porosity is varied. We observe non monotonic changes in total cell counts within different regions of the construct due to the interplay between porosity and cellular movement. We also analyze the emergent average cell speed for different initial scaffold porosities, observing higher average cell speed over the course of 30 days for the lowest initial scaffold porosity. This model provides a framework to further investigate how changes in biological parameters such as cell division and movement can change the cellular count and distribution in scaffolds of different initial porosity.

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Observation of chondrocyte aggregate formation and internal structure on micropatterned fibroin-coated surface

Cited by 4 publications

References 9 publications

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

The Silk, Versatile Material for Biological, Optical, and Electronic Fields: Review

A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting

A Hybrid Model of Cartilage Regeneration Capturing the Interactions Between Cellular Dynamics and Porosity

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