Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

Dawood, Kaiser E.; Morgan, Jess A. T.; Busfield, Frances; Srivastava, Mukesh; Fletcher, Taryn; Sambono, Jacqueline; Jackson, Louise A.; Venus, B.; Philbey, Adrian; Lew-Tabor, A.E.

doi:10.1016/j.ijppaw.2012.12.001

Cited by 19 publications

(22 citation statements)

References 48 publications

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“…Phylogenetic results obtained in this study were consistent with previous observations (Criado-Fornelio et al 2003, Zobba et al 2011, Schnittger et al 2012, Dawood et al 2013. Maximum likelihood trees generated with MEGA5 allowed the identification of six major Babesiadae clades (Fig.…”

Section: Fig 1 Detection Limit and Specificity Of The 18s Rrnasupporting

confidence: 91%

“…To identify nucleotide signatures of porcine isolates, the 18S rRNA sequence types obtained in this study were aligned with two sequences (accession nos. AY726557 and EU376017) representing the 18S rRNA gene variability of Ungulibabesids (Criado-Fornelio et al 2003, Dawood et al 2013.…”

Section: Sequencing 18s Rrna Variability and Phylogenetic Analysesmentioning

confidence: 99%

“…In particular, sequence type Swinetick1, found in symptomatic pigs and in ticks, showed 100% identity with HQ437690, whereas the other sequence types (Swine1-6, Tick1-3), found in asymptomatic subjects and in ticks were 99% similar to the same sequence. Alignments of the 10 18S sequence types together with sequences representative of Ungulibabesids (Criado-Fornelio et al 2003, Dawood et al 2013) allowed identification of 14 variable positions conserved among porcine isolates and therefore representing nucleotide signatures of this group of sequences (Table 3).…”

Section: Fig 1 Detection Limit and Specificity Of The 18s Rrnamentioning

confidence: 99%

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Molecular Epizootiology and Diagnosis of Porcine Babesiosis in Sardinia, Italy

Zobba

Nuvoli²,

Sotgiu³

et al. 2014

Vector-Borne and Zoonotic Diseases

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The recent characterization of the 18S ribosomal RNA (rRNA) of a pathogenic Babesia species in a domestic sow paved the way for establishing diagnostic and epidemiological tools for porcine babesiosis. Here, we developed the first specific Babesia sp. Suis PCR, and we applied this test to a panel of samples collected from animals living in a typical Mediterranean environment (Sardinia, Italy), including domestic pigs, wild boars, and ticks. In domestic pigs, PCR coupled with sequencing revealed an estimated Babesia infection frequency of 26.2% and the presence of distinct 18S sequence types. The different distribution of sequence types in symptomatic and asymptomatic subjects might suggest the existence of phylogenetically closely related strains with variable pathogenicity in pigs. Moreover, molecular identification of tick species indicated Rhipicephalus sanguineus and Rhipicephalus bursa as candidate vectors potentially involved in the transmission of this pathogen. Collectively, the data reveal the suitability of 18S rRNA PCR/sequencing for molecular diagnosis of porcine babesiosis and for large-scale investigations on the presence and geographical distribution of Babesia sp. Suis genetic variants.

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Section: Fig 1 Detection Limit and Specificity Of The 18s Rrnasupporting

confidence: 91%

Section: Sequencing 18s Rrna Variability and Phylogenetic Analysesmentioning

confidence: 99%

Section: Fig 1 Detection Limit and Specificity Of The 18s Rrnamentioning

confidence: 99%

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Molecular Epizootiology and Diagnosis of Porcine Babesiosis in Sardinia, Italy

Zobba

Nuvoli²,

Sotgiu³

et al. 2014

Vector-Borne and Zoonotic Diseases

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“…complex. For example, the recent description of a new species of Babesia in kangaroos (Dawood et al, 2013) may only be the 'tip of the iceberg' in describing new species as more molecular-based surveillance of wildlife parasites is undertaken.…”

Section: 'New' Parasite Zoonosesmentioning

confidence: 99%

Parasite zoonoses and wildlife: One health, spillover and human activity

Thompson

2013

International Journal for Parasitology

229

201

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This review examines parasite zoonoses and wildlife in the context of the One Health triad that encompasses humans, domestic animals, wildlife and the changing ecosystems in which they live. Human (anthropogenic) activities influence the flow of all parasite infections within the One Health triad and the nature and impact of resulting spillover events are examined. Examples of spillover from wildlife to humans and/or domestic animals, and vice versa, are discussed, as well as emerging issues, particularly the need for parasite surveillance of wildlife populations. Emphasis is given to Trypanosoma cruzi and related species in Australian wildlife, Trichinella, Echinococcus, Giardia, Baylisascaris, Toxoplasma and Leishmania.

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“…Species of Babesia occur in a wide range of domestic and wild mammals, B.bigemina and B. bovis in cattle representing the most economically important and pathogenic species in Australia (Dawood et al 2013). Although several species of Babesia have been described or identified in dasyurid, peramelid, potoroid and macropodid marsupials as well as the echidna, reports of clinical babesiosis in wildlife have been rare (Backhouse and Bolliger 1959;Mackerras 1959;Barker et al 1978;O'Donoghue and Adlard 2000;Paparini et al 2012;Dawood et al 2013;Donahoe et al 2015b).…”

Section: Piroplasmida Babesiamentioning

confidence: 99%

Wildlife parasitology in Australia: past, present and future

Spratt

Beveridge

2018

Aust. J. Zool.

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Wildlife parasitology is a highly diverse area of research encompassing many fields including taxonomy, ecology, pathology and epidemiology, and with participants from extremely disparate scientific fields. In addition, the organisms studied are highly dissimilar, ranging from platyhelminths, nematodes and acanthocephalans to insects, arachnids, crustaceans and protists. This review of the parasites of wildlife in Australia highlights the advances made to date, focussing on the work, interests and major findings of researchers over the years and identifies current significant gaps that exist in our understanding. The review is divided into three sections covering protist, helminth and arthropod parasites. The challenge to document the diversity of parasites in Australia continues at a traditional level but the advent of molecular methods has heightened the significance of this issue. Modern methods are providing an avenue for major advances in documenting and restructuring the phylogeny of protistan parasites in particular, while facilitating the recognition of species complexes in helminth taxa previously defined by traditional morphological methods. The life cycles, ecology and general biology of most parasites of wildlife in Australia are extremely poorly understood. While the phylogenetic origins of the Australian vertebrate fauna are complex, so too are the likely origins of their parasites, which do not necessarily mirror those of their hosts. This aspect of parasite evolution is a continuing area for research in the case of helminths, but remains to be addressed for many other parasitic groups.

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Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

Abstract: Graphical abstractHighlights► New Babesia is identified in kangaroo. ► The origin is unknown. ► Caused severe anaemia and death in the infected kangaroos.

Cited by 19 publications

References 48 publications

Molecular Epizootiology and Diagnosis of Porcine Babesiosis in Sardinia, Italy

Molecular Epizootiology and Diagnosis of Porcine Babesiosis in Sardinia, Italy

Parasite zoonoses and wildlife: One health, spillover and human activity

Wildlife parasitology in Australia: past, present and future

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