Objective Colorimetric Evaluation of Aging-related Articular Cartilage Degeneration

Satake, Yoshihiko; Takahashi, Kenji; Saito, Mitsuru; Shinro, Takai

doi:10.1272/jnms.jnms.2018_85-23

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“…Injury or overload of articular cartilage, particularly in elderly people, can induce OA (Bolduc et al 2018) and when it developes, a progressive degradation of ECM may lead to a complete destruction of cartilage (Jimenez et al 2018; Mora et al 2018). Aging or OA-related matrix degradation of articular cartilage is hardly treatable; only few methods are used in the clinical practice such as advanced glycation end products (AGE) treatment (Satake et al 2018) or corticosteroid injections (Mora et al 2018). With these interventions, decrease of joint inflammation and conservation of the status of articular cartilage can be the main goal.…”

Section: Discussionmentioning

confidence: 99%

Age-related alterations of articular cartilage in pituitary adenylate cyclase–activating polypeptide (PACAP) gene–deficient mice

et al. 2019

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Pituitary adenylate cyclase activating polypeptide (PACAP) is an evolutionarly conserved neuropeptide which is produced by various neuronal and non-neuronal cells, including cartilage and bone cells. PACAP has trophic functions in tissue development, and it also plays a role in cellular and tissue aging. PACAP takes part in the regulation of chondrogenesis, which prevents insufficient cartilage formation caused by oxidative and mechanical stress. PACAP knockout (KO) mice have been shown to display early aging signs affecting several organs. In the present work, we investigated articular cartilage of knee joints in young and aged wild-type (WT) and PACAP KO mice. A significant increase in the thickness of articular cartilage was detected in aged PACAP gene–deficient mice. Amongst PACAP receptors, dominantly PAC1 receptor was expressed in WT knee joints and a remarkable decrease was found in aged PACAP KO mice. Expression of PKA-regulated transcription factors, Sox5, Sox9 and CREB, decreased both in young and aged gene deficient mice, while Sox6, collagen type II and aggrecan expressions were elevated in young but were reduced in aged PACAP KO animals. Increased expression of hyaluronan (HA) synthases and HA-binding proteins was detected parallel with an elevated presence of HA in aged PACAP KO mice. Expression of bone related collagens (I and X) was augmented in young and aged animals. These results suggest that loss of PACAP signaling results in dysregulation of cartilage matrix composition and may transform articular cartilage in a way that it becomes more prone to degenerate.

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Section: Discussionmentioning

confidence: 99%

Age-related alterations of articular cartilage in pituitary adenylate cyclase–activating polypeptide (PACAP) gene–deficient mice

et al. 2019

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Objective Colorimetric Evaluation of Aging-related Articular Cartilage Degeneration

Abstract: This study developed an original colorimetric device with high validity. Browning of articular cartilage increased with age, but this study did not detect pentosidine-caused browning. Further study is needed to clarify the factors associated with browning of cartilage.

Cited by 1 publication

References 26 publications

Age-related alterations of articular cartilage in pituitary adenylate cyclase–activating polypeptide (PACAP) gene–deficient mice

Age-related alterations of articular cartilage in pituitary adenylate cyclase–activating polypeptide (PACAP) gene–deficient mice

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