Obesity modulates cell-cell interactions during ovarian folliculogenesis

Abstract

HighlightsSingle-cell transcriptome of folliculogenesis in regular diet-fed and obese mice ETCs represent higher activity in androgen synthesis under obese conditions Obesity contributes to the disproportion of GC subtypes under excessive androgen Enzalutamide alleviates defects in granulosa cells from obese mice

Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture

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Figure 5 UMAP (Uniform Manifold Approximation and Projection) plots colored by cell type markers The UMAP projection validates that oocytes, granulosa cells, and theca cells are highly enriched with our method. Adapted with permission from ( Long et al., 2022 ).

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“… The UMAP projection validates that oocytes, granulosa cells, and theca cells are highly enriched with our method. Adapted with permission from ( Long et al., 2022 ). …”

“…Within ovarian follicle, reciprocal dialog between somatic cells and oocyte is a prerequisite for oocyte development. In obese mouse models, we recently discovered that deregulated signaling in follicular somatic cells could compromise oocyte quality, explaining impacts of maternal obesity on offspring health ( Long et al., 2022 ).…”

“…This protocol provides an experiment framework and detailed methods combing single-cell RNA-seq and in vitro culture to test function of candidate factors identified by single-cell analyses. For further complete information, please refer to our recent work ( Gu et al., 2019 ; Long et al., 2022 ).…”

“…For complete details on the use and execution of this protocol, please refer to Gu et al. (2019) and Long et al. (2022) .…”