Obesity affects the proteome profile of periodontal ligament submitted to mechanical forces induced by orthodontic tooth movement in rats

Marcantonio, Camila Chierici; Lopes, Maria Eduarda Scordamaia; Mofatto, Luciana Souto; Salmon, Cristiane R.; Deschner, James; Nociti-Júnior, Francisco Humberto; Cirelli, Joni Augusto; Nogueira, Andressa Vilas Boas

doi:10.1016/j.jprot.2022.104616

Cited by 2 publications

(4 citation statements)

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“…Moreover, in another study, we observed the detrimental effects of obesity on the protein abundance of the PDL of rats exposed to orthodontic movement. It suggested that the chronic inflammation in obesity might interfere with the aseptic inflammation induced by the mechanical loading by the increase of vinculin, osteopontin, and cathepsin D [ 24 ]. Therefore, it is exciting to see how proteomic analysis enables us to observe that a systemic condition or disease can interfere with a microenvironment, such as PDL.…”

Section: Discussionmentioning

confidence: 99%

“…The animals received a standard laboratory diet (Labina/Purina, Ribeirão Preto, Brazil) or a high-fat diet in addition to water ad libitum. They were randomly divided into two experimental groups: control animals (n = 3) and obese animals (n = 5), which comprised animals subjected to obesity induction by a high-fat diet, as previously described [ 23 , 24 ]. This high-fat diet had about 3.82 kcal/g and consisted of a mixture of standard rat chow and milk chocolate, peanuts, and sweet biscuits in a 3:2:2:1 ratio and contained 20 g of protein, 20 g of total fat, 48 g of carbohydrates, and 4 g of fiber per 100 g of diet.…”

Section: Methodsmentioning

confidence: 99%

“…The protein abundance through proteomics enables the identification of the main proteins present in a sample and also differentially abundant proteins in different samples. Lately, proteomic analysis has been used widely in different challenged oral cells, tissues, and fluids, such as enamel [ 17 ], pulp [ 18 , 19 ], dental cementum [ 20 , 21 , 22 ], PDL [ 23 , 24 ], alveolar bone [ 22 ], periodontal ligament cells [ 25 , 26 ], gingival fibroblasts [ 27 ], gingival tissue [ 28 , 29 ], saliva [ 30 ], and gingival crevicular fluid [ 31 ], enabling researchers to better understand biological processes. Therefore, characterizing the PDL protein profile in obesity conditions becomes important in identifying possible biological markers different from those in health conditions.…”

Section: Introductionmentioning

confidence: 99%

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Obesity Modifies the Proteomic Profile of the Periodontal Ligament

Nogueira

Lopes

Marcantonio

et al. 2023

IJMS

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This study aimed to assess the obesity effects on the proteomic profile of the periodontal ligament of rats submitted to obesity induction by a high-fat diet. Eight Holtzman rats were divided into control (n = 3) and obese (n = 5) groups. The maxillae were histologically processed for laser capture microdissection of the periodontal ligament of the first maxillary molars. Peptide mixtures were analyzed by LC-MS/MS. A total of 1379 proteins were identified in all groups. Among them, 335 (24.30%) were exclusively detected in the obese group, while 129 (9.35%) proteins were uniquely found in the control group. Out of the 110 (7.98%) differentially abundant proteins, 10 were more abundant and 100 had decreased abundance in the obese group. A gene ontology analysis showed some proteins related to obesity in the “extracellular exosome” term among differentially identified proteins in the gene ontology cellular component terms Prelp, Sec13, and Sod2. These three proteins were upregulated in the obese group (p < 0.05), as shown by proteomic and immunohistochemistry analyses. In summary, our study presents novel evidence that the proteomic profile of the periodontal ligament is altered in experimental obesity induction, providing a list of differentially abundant proteins associated with obesity, which indicates that the periodontal ligament is responsive to obesity.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Obesity Modifies the Proteomic Profile of the Periodontal Ligament

Nogueira

Lopes

Marcantonio

et al. 2023

IJMS

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“…Alveolar bone remodeling occurs during bone resorption, and cancellous bone is the main site of bone remodeling [ 1 , 2 ]. Previous studies have shown that [ 3 , 4 , 5 , 6 ] the direction and speed of tooth movement are mainly related to the stress distribution in CBAB under stress. Therefore, in order to quantify the relationship between orthodontic force and tooth movement, it is necessary to further clarify the stress response state of CBAB under stress so as to help improve the clinical treatment effect [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanical Behavior of Human Cancellous Bone in Alveolar Bone under Uniaxial Compression and Creep Tests

Liu

et al. 2022

Materials

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In the process of orthodontic treatment, the remodeling of cancellous bone in alveolar bone (in this paper, cancellous bone in alveolar bone is abbreviated as CBAB) is key to promoting tooth movement. Studying the mechanical behavior of CBAB is helpful to predict the displacement of teeth and achieve the best effect of orthodontic treatment. Three CBAB samples were cut from alveolar bone around the root apex of human teeth. A uniaxial compression test was used to study the transient elastic properties of CBAB. A creep test was used to study the time-dependent viscoelastic properties of CBAB. Both tests were carried out at the loading rates of 0.02 mm/min, 0.1 mm/min and 0.5 mm/min. The results revealed that CBAB is a nonlinear viscoelastic and hyperelastic material. The stress–strain curve obtained from the uniaxial compression test could be divided into three stages: the collapse stage of the front section, the exponential stage of the middle section and the almost linear stage of the rear end. According to the strain–time curve obtained from the compression creep test, a trend of increasing strain over time was relatively obvious within the first 30 s. After 200 s, the curve gradually tended to plateau. Four hyperelastic models and three viscoelastic models were used to fit the test data. Finally, the fifth-order polynomial hyperelastic model (coefficient of determination “R2 > 0.999”) was used to describe the hyperelastic properties of CBAB, and the seven-parameter model of the generalized Kelvin modified model (“R2 > 0.98”) was used to describe the viscoelastic properties of CBAB.

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Obesity affects the proteome profile of periodontal ligament submitted to mechanical forces induced by orthodontic tooth movement in rats

Cited by 2 publications

References 84 publications

Obesity Modifies the Proteomic Profile of the Periodontal Ligament

Obesity Modifies the Proteomic Profile of the Periodontal Ligament

Mechanical Behavior of Human Cancellous Bone in Alveolar Bone under Uniaxial Compression and Creep Tests

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