Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism

Śmieszek, Agnieszka; Marcinkowska, Klaudia; Pielok, Ariadna; Sikora, Mateusz; Valihrach, Lukás; Carnevale, E. M.; Marycz, Krzysztof

doi:10.3390/cells11091437

Cited by 6 publications

(15 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deteriorated oxidative status of Eca EPCs significantly affects their pro-regenerative function related to proliferation and multilineage differentiation properties. 8 Oxidative stress of stromal cells accompanied by tissue inflammation also underlie the pathophysiology of endometriosis. 24,25 Different agents were applied to target endometrial cell oxidative stress, improve endometrium regeneration and treat endometriosis.…”

Section: Discussionmentioning

confidence: 99%

“…Cells were isolated accordingly to the protocol published previously. 8 The cultures were maintained in a complete growth medium (CGM) composed of medium (DMEM/F12, Sigma-Aldrich/ Merck, Poznan, Poland) supplemented by 15% of foetal bovine serum (Sigma-Aldrich/Merck, Poznan, Poland) and 1% of antibiotics (Pen Strep, Sigma-Aldrich/Merck, Poznan, Poland). The cells were cultured in aseptic conditions at 37°C with humidity established at 95% and CO 2 at 5%.…”

Section: Experimental Culture Conditionsmentioning

confidence: 99%

“…The staining procedure was described in detail previously. 8 Obtained images were analysed with Fiji software (ImageJ…”

Section: Evaluation Of β -Lg Influence On Epcs Cytophysiology-oxidati...mentioning

confidence: 99%

“…Previously, we have shown that obesity-induced oxidative stress affects the metabolism of equine endometrial progenitor cells (Eca EPCs), decreasing the dynamics of the mitochondrial network and deteriorating the regenerative potential of cells related to proliferative activity and multipotency maintenance. 8 Moreover, oxidative stress impairs the decidualization of endometrial stromal cells (ECSs), inducing their apoptosis and declining mitochondrial membrane potential. 11 On the contrary, mounting evidence indicates that resistance to oxidative stress and intracellular reactive oxygen species (ROS) levels increase during the decidualization of ESCs.…”

mentioning

confidence: 99%

See 3 more Smart Citations

β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations

Data

Marcinkowska

Buś

et al. 2023

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

β-Lactoglobulin (β-LG) is a small globular protein consisting of 162 amino acid residues that belong to lipocalins, which are potential regulators of cellular communication. [1][2][3][4] The β-LG has an antioxidative activity, but its potential does not exceed that of vitamin E and probucol. 2 Moreover, β-LG is an efficient transporter of molecules with antioxidant and anticancer properties, including phenolic compounds, vitamins and many others. 1,[5][6][7][8] The β-LG is a major component of ruminant milk of most species, except for humans, guinea pigs, rabbits and rodents. Thus, the biological role of β-LG is considered regarding its nutritional profile and/

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Experimental Culture Conditionsmentioning

confidence: 99%

“…The staining procedure was described in detail previously. 8 Obtained images were analysed with Fiji software (ImageJ…”

Section: Evaluation Of β -Lg Influence On Epcs Cytophysiology-oxidati...mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations

Data

Marcinkowska

Buś

et al. 2023

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

show abstract

“…It should be noted this was a SNP identified in mares not abortus material, so likely acts on the embryonic environment via the endometrium or immune function although this remains to be explored. Altered expression of p53 has been demonstrated in equine endometrial progenitor cells in obsese mares who in turn present with suboptimal endometrial function (Smieszek et al 2022).…”

Section: Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 99%

Lethal variants of equine pregnancy: is it the placenta or foetus leading the conceptus in the wrong direction?

Shilton

Kahler²,

Roach³

et al. 2022

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Embryonic and foetal loss remain one of the greatest challenges in equine reproductive health with 5-10% of established day 15 pregnancies and a further 5-10% of day 70 pregnancies failing to produce a viable foal. The underlying reason for these losses is variable but ultimately most cases will be attributed to pathologies of the environment of the developing embryo and later foetus, or a defect intrinsic to the embryo itself that leads to lethality at any stage of gestation right up to birth. Historically, much research has focused on the maternal endometrium, endocrine and immune responses in pregnancy and pregnancy loss, as well as infectious agents such as pathogens, and until recently very little was known about the both small and large genetic variants associated with reduced foetal viability in the horse. In this review, we first introduce key aspects of equine placental and foetal development. We then discuss incidence, risk factors and causes of pregnancy loss, with the latter focusing on genetic variants described to date that can impact equine foetal viability.

show abstract

Identification and characterization of stromal-like cells with CD207+/low CD1a+/low phenotype derived from histiocytic lesions – a perspective in vitro model for drug testing

Śmieszek,

Marcinkowska,

Małas

et al. 2024

BMC Cancer

View full text Add to dashboard Cite

Background Histiocytoses are rare disorders manifested by increased proliferation of pathogenic myeloid cells sharing histological features with macrophages or dendritic cells and accumulating in various organs, i.a., bone and skin. Pre-clinical in vitro models that could be used to determine molecular pathways of the disease are limited, hence research on histiocytoses is challenging. The current study compares cytophysiological features of progenitor, stromal-like cells derived from histiocytic lesions (sl-pHCs) of three pediatric patients with different histiocytoses types and outcomes. The characterized cells may find potential applications in drug testing. Methods Molecular phenotype of the cells, i.e. expression of CD1a and CD207 (langerin), was determined using flow cytometry. Cytogenetic analysis included GTG-banded metaphases and microarray (aCGH) evaluation. Furthermore, the morphology and ultrastructure of cells were evaluated using a confocal and scanning electron microscope. The microphotographs from the confocal imaging were used to reconstruct the mitochondrial network and its morphology. Basic cytophysiological parameters, such as viability, mitochondrial activity, and proliferation, were analyzed using multiple cellular assays, including Annexin V/7-AAD staining, mitopotential analysis, BrdU test, clonogenicity analysis, and distribution of cells within the cell cycle. Biomarkers potentially associated with histiocytoses progression were determined using RT-qPCR at mRNA, miRNA and lncRNA levels. Intracellular accumulation of histiocytosis-specific proteins was detected with Western blot. Cytotoxicyty and IC50 of vemurafenib and trametinib were determined with MTS assay. Results Obtained cellular models, i.e. RAB-1, HAN-1, and CHR-1, are heterogenic in terms of molecular phenotype and morphology. The cells express CD1a/CD207 markers characteristic for dendritic cells, but also show intracellular accumulation of markers characteristic for cells of mesenchymal origin, i.e. vimentin (VIM) and osteopontin (OPN). In subsequent cultures, cells remain viable and metabolically active, and the mitochondrial network is well developed, with some distinctive morphotypes noted in each cell line. Cell-specific transcriptome profile was noted, providing information on potential new biomarkers (non-coding RNAs) with diagnostic and prognostic features. The cells showed different sensitivity to vemurafenib and trametinib. Conclusion Obtained and characterized cellular models of stromal-like cells derived from histiocytic lesions can be used for studies on histiocytosis biology and drug testing.

show abstract

Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism

Cited by 6 publications

References 87 publications

β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations

β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations

Lethal variants of equine pregnancy: is it the placenta or foetus leading the conceptus in the wrong direction?

Identification and characterization of stromal-like cells with CD207+/low CD1a+/low phenotype derived from histiocytic lesions – a perspective in vitro model for drug testing

Contact Info

Product

Resources

About