Obese <i>Neotomodon alstoni</i> mice exhibit sexual dimorphism in the daily profile of circulating melatonin and clock proteins PER1 and BMAL1 in the hypothalamus and peripheral oscillators

Arellanes-Licea, Elvira del Carmen; Pérez-Mendoza, Moisés; Carmona-Castro, Agustín; Dı́az-Muñoz, Mauricio; Miranda‐Anaya, Manuel

doi:10.1080/07420528.2020.1860999

Cited by 5 publications

(4 citation statements)

References 56 publications

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“…Energy expenditure (EE) in kCal/min −1 ( Figure 1D ) was clearly reduced in obese mice. In previous studies, we observed that obese N. alstoni mice show half the amount of locomotor activity during the night and sometimes more activity during noon than lean mice ( 27 , 37 ), results that are consistent with a reduced EE observed during the night in the present work. Nevertheless, during the rest phase, EE in obese mice is still reduced compared to lean mice, indicating that metabolic use of energy during rest is also different, as observed in body temperature results ( Figure 3A ) as well as it may be related with the enhanced food intake observed during the day in obese mice ( Figure 2A ).…”

Section: Discussionsupporting

confidence: 93%

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Obesity in male volcano mice Neotomodon alstoni affects the daily rhythm of metabolism and thermoregulation

Herrera-García

Pérez-Mendoza²,

Arellanes-Licea³

et al. 2022

Front. Nutr.

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The mouse N. alstoni spontaneously develops the condition of obesity in captivity when fed regular chow. We aim to study the differences in metabolic performance and thermoregulation between adult lean and obese male mice. The experimental approach included indirect calorimetry using metabolic cages for VO2 intake and VCO2 production. In contrast, the body temperature was measured and analyzed using intraperitoneal data loggers. It was correlated with the relative presence of UCP1 protein and its gene expression from interscapular adipose tissue (iBAT). We also explored in this tissue the relative presence of Tyrosine Hydroxylase (TH) protein, the rate-limiting enzyme for catecholamine biosynthesis present in iBAT. Results indicate that obese mice show a daily rhythm persists in estimated parameters but with differences in amplitude and profile. Obese mice presented lower body temperature, and a low caloric expenditure, together with lower VO2 intake and VCO2 than lean mice. Also, obese mice present a reduced thermoregulatory response after a cold pulse. Results are correlated with a low relative presence of TH and UCP1 protein. However, qPCR analysis of Ucp1 presents an increase in gene expression in iBAT. Histology showed a reduced amount of brown adipocytes in BAT. The aforementioned indicates that the daily rhythm in aerobic metabolism, thermoregulation, and body temperature control have reduced amplitude in obese mice Neotomodon alstoni.

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Section: Discussionsupporting

confidence: 93%

“…Total RNA was isolated from Neotomodon alstoni from iBAT, quickly dissected and frozen in dry ice and kept at −80°C until further processing ( 37 ). We use the TRIzol™ Reagent (Invitrogen Life Technologies, Carlsbad, CA) following the manufacturer's instructions, with minor modifications ( 38 , 39 ).…”

Section: Methodsmentioning

confidence: 99%

Obesity in male volcano mice Neotomodon alstoni affects the daily rhythm of metabolism and thermoregulation

Herrera-García

Pérez-Mendoza²,

Arellanes-Licea³

et al. 2022

Front. Nutr.

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“…PER1 has a role in feeding and active behavior in mammals and is controlled by circadian rhythms (Kim et al, 2020). From a non-tumor perspective, PER1 is related to conditions such as Parkinson's disease, obesity, sleep, drug resistance, and premature ovarian insufficiency (Zheng et al, 2019;Delgado-Lara et al, 2020;EmeklI et al, 2020;Mabrouk et al, 2020;Arellanes-Licea et al, 2021). PER1 enhances the activity of GPX through the interaction of PER1 and GPX1 in the cytoplasm, thereby improving the oxidative phosphorylation efficiency of mitochondria (Sun et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer

et al. 2021

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Background: Period circadian protein homolog 1 (PER1) is an important component of the biorhythm molecular oscillation system and plays an important part in the development and progression of mammalian cancer. However, the correlations of PER1 with prognosis and tumor-infiltrating lymphocytes in ovarian cancer (OV) remain unclear.Methods: The Oncomine and TIMER databases were used to examine the expression of PER1 in OV. Kaplan–Meier Plotter and PrognoScan were used to evaluate the relationship between PER1 and prognosis. Kaplan–Meier Plotter was used to analyze the relationships between PER1 and clinicopathological features of OV patients. The relationship between PER1 expression and immune infiltration in OV was investigated using the TIMER database and CIBERSORT algorithm. The STRING database was used to analyze PER1-related protein functional groups, the GeneMANIA online tool was used to analyze gene groups with similar functions to those of PER1, and Network Analyst was used to identify transcription factors that regulate PER1. The correlation between PER1 and immunoinvasion of OV was analyzed using TIMER. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect PER1 expression.Results: PER1 was differentially expressed in different cancer tissues, and its expression in various OV subtypes was lower than that in normal ovarian tissue. OV patients with low PER1 expression had a reduced overall survival rate. Decreased PER1 expression in stage 1 and stage 1+2 OV patients was related to poor prognosis, while increased PER1 expression in stage 3+4 patients and TP53 mutation were related to poor overall survival and progression-free survival. We identified eight genes whose expression was strongly correlated with that of PER1, as well as four transcription factors that regulate PER1. In OV, PER1 expression levels were positively correlated with infiltration levels of cells including neutrophils, regulatory T cells, and M2 macrophages, and closely related to a variety of immune markers. Reduced expression of PER1 was significantly associated with poor overall survival.Conclusion: These findings suggest that PER1 could be used as a prognostic biomarker to determine prognosis and immune infiltration in OV patients.

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“…Furthermore, melatonin not only enhances autophagy through BMAL1 but also improves cerebral ischemia–reperfusion in diabetic mice [ 65 ]. It can also increase the expression of clock proteins to affect endocrine status and improve obesity in mice [ 66 ]. Blocking the BMAL1 gene with siRNA reduced the effects of melatonin on rooster circadian rhythms, implying that melatonin primarily functions through BMAL1 [ 67 ], with BMAL1 at the apex of the circadian clock feedback pathway in the avian retina [ 68 ].…”

Section: Melatonin Regulates Circadian Rhythms In Cattlementioning

confidence: 99%

Role of Melatonin in Bovine Reproductive Biotechnology

Li,

Zhang,

Zhou

et al. 2023

Molecules

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Melatonin has profound antioxidant activity and numerous functions in humans as well as in livestock and poultry. Additionally, melatonin plays an important role in regulating the biological rhythms of animals. Combining melatonin with scientific breeding management has considerable potential for optimizing animal physiological functions, but this idea still faces significant challenges. In this review, we summarized the beneficial effects of melatonin supplementation on physiology and reproductive processes in cattle, including granulosa cells, oocytes, circadian rhythm, stress, inflammation, testicular function, spermatogenesis, and semen cryopreservation. There is much emerging evidence that melatonin can profoundly affect cattle. In the future, we hope that melatonin can not only be applied to cattle, but can also be used to safely and effectively improve the efficiency of animal husbandry.

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Obese Neotomodon alstoni mice exhibit sexual dimorphism in the daily profile of circulating melatonin and clock proteins PER1 and BMAL1 in the hypothalamus and peripheral oscillators

Cited by 5 publications

References 56 publications

Obesity in male volcano mice Neotomodon alstoni affects the daily rhythm of metabolism and thermoregulation

Obesity in male volcano mice Neotomodon alstoni affects the daily rhythm of metabolism and thermoregulation

PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer

Role of Melatonin in Bovine Reproductive Biotechnology

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