Oat-based postbiotics ameliorate high-sucrose induced liver injury and colitis susceptibility by modulating fatty acids metabolism and gut microbiota

Song, Wei; Wen, Ruixue; Liu, Tianqi; Zhou, Lanqi; Wang, Guoze; Dai, Xiaoshuang; Shi, Lin

doi:10.1016/j.jnutbio.2023.109553

Cited by 6 publications

(5 citation statements)

References 77 publications

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“…Afterward, 1 mL of supernatant was collected and dried using nitrogen and was resolubilized for analysis using a gas chromatography–mass spectrometer (GC-MS, Agilent Technologies, Santa Clara, California, USA) by Shanghai Applied Protein Technology Biotechnology Co., China. Detailed analytical protocols have been published elsewhere …”

Section: Methodsmentioning

confidence: 99%

“…Detailed analytical protocols have been published elsewhere. 12 2.9. 16s rRNA Sequencing and Short-Chain Fatty Acid Assessments.…”

Section: 3mentioning

confidence: 99%

“…7−9 As an alternative to live probiotics, inactivated bacteria and microbialderived metabolites have been demonstrated to be comparably effective 10 and offer great advantages over live probiotics in light of improved safety and processability. 11,12 For instance, heatinactivated L. reuteri has shown to alleviate high-fat-diet (HFD)induced hepatic steatosis in rats 13 and was recently found to protect against cardiac damage associated with diabetes. 14 Singh et al found that the cell surface and extracellular proteins of L. reuteri could improve the intestinal epithelial barrier function.…”

Section: Introductionmentioning

confidence: 99%

“…reuteri) is a commensal intestinal species that resides in the gastrointestinal tracts of humans and animals, which is recognized as a probiotic showing multiple health effects, e.g., anti-inflammation, improvement on immunity function, lipid metabolism, and liver health. , The oral use of probiotics including L. reuteri in dietary supplements, infant formula formulations, and medical devices is widespread, but the safety profile with the use of live probiotics is still a matter of debate, particularly in vulnerable populations. − As an alternative to live probiotics, inactivated bacteria and microbial-derived metabolites have been demonstrated to be comparably effective and offer great advantages over live probiotics in light of improved safety and processability. , For instance, heat-inactivated L. reuteri has shown to alleviate high-fat-diet (HFD)-induced hepatic steatosis in rats and was recently found to protect against cardiac damage associated with diabetes .…”

Section: Introductionmentioning

confidence: 99%

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Polygonatum kingianum Polysaccharides Enhance the Preventive Efficacy of Heat-Inactivated Limosilactobacillus reuteri WX-94 against High-Fat-High-Sucrose-Induced Liver Injury and Gut Dysbacteriosis

Liu,

Zhou,

et al. 2024

J. Agric. Food Chem.

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Limosilactobacillus reuteri (L. reuteri) is an efficacious probiotic that could reduce inflammation and prevent metabolic disorders. Here, we innovatively found that Polygonatum kingianum polysaccharides (PKP) promoted proliferation and increased stability of L. reuteri WX-94 (a probiotic strain showing anti-inflammation potentials) in simulated digestive fluids in vitro. PKP was composed of galactose, glucose, mannose, and arabinose. The cell-free supernatant extracted from L. reuteri cultured with PKP increased ABTS•+, DPPH•, and FRAP scavenging capacities compared with the supernatant of the medium without PKP and increased metabolites with health-promoting activities, e.g., 3-phenyllactic acid, indole-3-lactic acid, indole-3-carbinol, and propionic acid. Moreover, PKP enhanced alleviating effects of heat-inactivated L. reuteri on high-fat-high-sucrose-induced liver injury in rats via reducing inflammation and regulating expressions of protein and genes involved in fatty acid metabolism (such as HIF1-α, FAβO, CPT1, and AMPK) and fatty acid profiles in liver. Such benefits correlated with its prominent effects on enriching Lactobacillus and short-chain fatty acids while reducing Dubosiella, Fusicatenilacter, Helicobacter, and Oscillospira. Our work provides novel insights into the probiotic property of PKP and emphasizes the great potential of the inactivated L. reuteri cultured with PKP in contracting unhealthy diet-induced liver dysfunctions and gut dysbacteriosis.

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Section: Methodsmentioning

confidence: 99%

“…Detailed analytical protocols have been published elsewhere. 12 2.9. 16s rRNA Sequencing and Short-Chain Fatty Acid Assessments.…”

Section: 3mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polygonatum kingianum Polysaccharides Enhance the Preventive Efficacy of Heat-Inactivated Limosilactobacillus reuteri WX-94 against High-Fat-High-Sucrose-Induced Liver Injury and Gut Dysbacteriosis

Liu,

Zhou,

et al. 2024

J. Agric. Food Chem.

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“…Detailed information for RT-qPCR and western blot analysis has been presented previously. 37 The primer sequences were synthesized by DynaPro Biotech (Beijing, China) and are presented in Table S1. The product information on the antibodies is shown in Table S2.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Eurotium cristatum Fermented Thinned Young Apple and Mechanisms Underlying Its Alleviating Impacts on Experimental Colitis

Song,

Zhou,

Liu

et al. 2024

J. Agric. Food Chem.

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A hundred million tons of young apples are thinned and discarded in the orchard per year, aiming to increase the yield and quality of apples. We fermented thinned young apples using a potential probiotic fungus, Eurotium cristatum, which notably disrupted the microstructure of raw samples, as characterized by the scanning electron microscope. Fermentation substantially altered the metabolite profiles of samples, which are predicted to alleviate colitis via regulating inflammatory response and response to lipopolysaccharide by using network pharmacology analysis. In vivo, oral gavage of water extracts of E. cristatum fermented young apples (E.YAP) effectively alleviated DSS-induced colitis, restored the histopathology damage, reduced the levels of inflammatory cytokines, and promoted colonic expressions of tight junction proteins. Moreover, E.YAP ameliorated gut dysbacteriosis by increasing abundances of Lactobacillus,Blautia, Muribaculaceae, and Prevotellaceae_UCG-001 while inhibiting Turicibacter, Alistipes, and Desulfovibrio. Importantly, E.YAP increased colonic bile acids, such as CA, TCA, DCA, TUDCA, and LCA, thereby alleviating colitis via PXR/NF-κB signaling. Furthermore, a synbiotic combination with Limosilactobacillus reuteri WX-94, a probiotic strain isolated from feces of healthy individuals with anti-inflammatory properties, augmented anticolitis capacities of E.YAP. Our findings demonstrate that E.YAP could be a novel, potent, food-based anti-inflammatory prebiotic for relieving inflammatory injuries.

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Multi-omics insights into anti-colitis benefits of the synbiotic and postbiotic derived from wheat bran arabinoxylan and Limosilactobacillus reuteri

Zhou,

Song,

Liu

et al. 2024

International Journal of Biological Macromolecules

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Oat-based postbiotics ameliorate high-sucrose induced liver injury and colitis susceptibility by modulating fatty acids metabolism and gut microbiota

Cited by 6 publications

References 77 publications

Polygonatum kingianum Polysaccharides Enhance the Preventive Efficacy of Heat-Inactivated Limosilactobacillus reuteri WX-94 against High-Fat-High-Sucrose-Induced Liver Injury and Gut Dysbacteriosis

Polygonatum kingianum Polysaccharides Enhance the Preventive Efficacy of Heat-Inactivated Limosilactobacillus reuteri WX-94 against High-Fat-High-Sucrose-Induced Liver Injury and Gut Dysbacteriosis

Characterization of Eurotium cristatum Fermented Thinned Young Apple and Mechanisms Underlying Its Alleviating Impacts on Experimental Colitis

Multi-omics insights into anti-colitis benefits of the synbiotic and postbiotic derived from wheat bran arabinoxylan and Limosilactobacillus reuteri

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