Oak and chestnut wood extracts enhance the intestinal gut barrier function in a porcine in vitro cell model

Novak, Božidar; Springler, Alexandra; Stelzer, Julian E.; Mayer, Eric; Schatzmayr, Gerd

doi:10.1055/s-0036-1596334

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“…The authors suggested that the protective effect might involve its anti‐oxidative activity and/or the inhibition of phosphorylated p38 MAP activity (Chuenkitiyanon, Pengsuparp, & Jianmongkol, ; Chuenkitiyanon, Vardhanabhuti, & Jianmongkol, ). Our own preliminary experiments showed that an oak wood extract enhanced the TEER both in unchallenged cells and in cells treated with a stressor other than the calcium switch assay (Novak, Springler, Stelzer, Mayer, & Schatzmayr, ). Considering the results of the present study in light of previously published data, it might be suggested that oak bark extract and its active ingredient quercetin can positively influence the barrier function but that a calcium switch might not be the best model for testing oak bark extract or other polyphenol‐rich samples with chelating properties.…”

Section: Discussionmentioning

confidence: 97%

Influence of phytogenics on recovery of the barrier function of intestinal porcine epithelial cells after a calcium switch

Bachinger¹,

Mayer²,

Kaschubek

et al. 2018

Animal Physiology Nutrition

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Background The gut barrier is essential for animal health as it prevents the passage of potentially harmful foreign substances. The epithelial tight junctions support the intestinal barrier and can be disrupted by stress caused, for example, by pathogens or dietary or environmental factors, predisposing the host to disease. In animal husbandry, phytogenics (plant‐derived feed additives) are used to support and maintain growth, feed efficiency and health. Therefore, several phytogenics were tested in vitro for their influence on the barrier function recovery of intestinal porcine epithelial cells (IPEC‐J2) after disruption, particularly on the abundance of tight junction proteins. Results IPEC‐J2 treated with 1,000 µg/ml liquorice root extract, 80 µg/ml plant powder mix, or 80 µg/ml angelica root powder showed significantly higher trans‐epithelial electric resistance (TEER) 24 hr after tight junction disruption via a calcium switch assay than the control. In contrast, cells treated with 1,000 µg/ml oak bark extract showed a significantly lower TEER after 6 hr but not at later time points. The increased TEER caused by the liquorice root extract correlated with an increase in the abundance of the tight junction protein claudin‐4. Conclusions This study suggests potential beneficial effects of liquorice and angelica root extracts on the gut barrier function when used as feed additives for livestock. Further studies, especially in vivo, are necessary to confirm these findings.

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Section: Discussionmentioning

confidence: 97%