OA01.47. Phytochemical study and evaluation of antileukemic activity of ripe fruit of Opuntia elatior Mill

Abstract

Purpose:Opuntia elatior Mill. (Family: Cactacea) is a xerophytic perennial bushy shrub, 1-3 m tall. It is native of Caribbean, Central America and northern South America, but actually it is fully naturalized in many part of the world like Mediterranean, Australia, Africa and India. Ripe fruits of this plant contain red pigment i.e. betanin which is betacyanin pigment. It is recently documented that natural food colors such as betanin can inhibit the cell proliferation of a variety of human tumor cells. In view of its traditional use and chemical composition, Opuntia elatior Mill. fruit was selected for its in vitro antileukemic and antioxidant activity.Method:The fruits of Opuntia elatior after collection, authentication and drying was extracted with hydroalcoholic solvent using maceration method. The present study aimed to determine the preliminary phytochemical evaluation, quantitative estimation of Betanin pigment, total phenolic, flavonoid and flavonone content with spectrophotometric methods, evaluation of antioxidant and cytotoxicity study of the hydroalcholic extract of Opuntia elatior on K-562 cell line (Human chronic myelocytic leukemia) by MTT assay method.Result:The preliminary phytochemical evaluation showed the presence of carbohydrates, flavonoids, betanin pigment, tannins, sterols, proteins, pectin, citric acid and vitamin C. The total phenolic, flavonoid, flavonone and betanin content in extract were found to be 52.76 mg/g, 39.22 mg/g, 9.60 mg/g and 48.10 mg/100ml of extract respectively. Evaluation of antioxidant activity was carried out by DPPH free radical scavenging method. It showed significant activity compared with the standard. IC50 values for extract of O.elatior and Vitamin C was found to be 88.16 g/ml and 62.83 g/ml respectively.Conclusion:Its cytotoxic evalution showed a potent action against to K-562 (Human chronic myelocytic leukemia) cell line. It was found that hydroalcoholic extract of the fruits of Opuntia elatior Mill. have persuasive antioxidant activity and promising antileukemic activity.

Effect of Opuntia elatior Mill. (Nagaphani) in the management of Pandu Roga w. s. r to iron deficiency anemia; an open labeled randomized standard controlled clinical trial

“…The presence of iron and ascorbic acid (Vitamin C) in trial drug[ 20 ] which reduces ferric iron to ferrous iron and remains soluble even at neutral pH and is better absorbed. Even when the diet is poor in iron, Vitamin C supplements with each meal enhances iron absorption.…”

Opuntia Elatior Mill.–its Phytochemistry and Pharmacological Properties-a Review

“…The cytotoxic evaluation showed a potent action against K562 (Human chronic myelocytic leukemia) cell line. Hydroalcoholic extract of the fruits of OE have persuasive antioxidant activity and promising antileukemic activity [17]. LC50 value of OE in aqueous fraction showed more cytotoxic activity than Tamoxifen.…”

“…Ethanolic extract of OE contains alkaloids, carbohydrates, [5,6,[15][16][17] fats, [5,6,[15][16][17] oils, flavonoids, phenolics, tannins, steroids, saponins, [6,[15][16][17] proteins, [5,6] betalains, [5,6,17] and Vitamin C. [5] Major constituents of OE are carbohydrates (glucose, sucrose, reducing hexose monosaccharide ie. Glucose and galactose), polyphenols (vannilic, syringic and protocatechuric phenolic acids), flavonoids (flavonoids and flavonone) and betalains (betacyanins type of color pigments).…”

“…Methanolic extract exhibited the highest antioxidant activity at 54.10% and the lowest antioxidant activity is exhibited by the hexanoic extract at 45.66% and the distilled water at 50.40% of antioxidant activity through Dimethyl sulfoxide (DPPH) assay [5]. IC50 values for extract of OE and Vitamin C was found to be 88.16 g/ml and 62.83 g/ml respectively [17]. Antioxidant property in aqueous extract of OE was determined by Phenolic content assay, Flavonoid content assay and Reducing power assay.…”