O xidative stress differentially impacts male and female bovine embryos depending on the culture medium and the stress condition

Dallemagne, Matthew; Ghys, Emmanuelle; Schrevel, Catalina De; Mwema, Ariane; Troy, Delphine De; Rasse, Catherine; Donnay, Isabelle

doi:10.1016/j.theriogenology.2018.05.020

Cited by 12 publications

(11 citation statements)

References 52 publications

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“…The negative effect of high oxygen level in terms of cell number was more pronounced in female embryos than male counterparts. This fact is in agreement with Dallemagne et al (), who showed that exposing bovine embryos to OS from Days 5 to 7 in presence of FCS but not bovine serum albumin supplemented with insulin, transferrin and selenium (BSA‐ITS) resulted in a reduction of total cell number and increasing apoptosis in female embryos compared with male counterparts. Moreover, higher cell number was observed in male embryos than female cultured under 5% oxygen level in presence FCS or BSA‐ITS, which was not in agreement with the current study (Dallemagne et al, ).…”

Section: Discussionsupporting

confidence: 91%

“…This fact is in agreement with Dallemagne et al (), who showed that exposing bovine embryos to OS from Days 5 to 7 in presence of FCS but not bovine serum albumin supplemented with insulin, transferrin and selenium (BSA‐ITS) resulted in a reduction of total cell number and increasing apoptosis in female embryos compared with male counterparts. Moreover, higher cell number was observed in male embryos than female cultured under 5% oxygen level in presence FCS or BSA‐ITS, which was not in agreement with the current study (Dallemagne et al, ). Supporting to our results, no significant difference in total cell numbers was observed between male and female bovine embryos cultured under low oxygen tension (Siqueira & Hansen, ), which indicates the contribution of culture media in the variation between male and female embryos under either normal or stress conditions.…”

Section: Discussionsupporting

confidence: 91%

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Sexual dimorphic expression and release of transcription factors in bovine embryos exposed to oxidative stress

Taqi

Saeed‐Zidane

Gebremedhn

et al. 2019

Molecular Reproduction Devel

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Sexually dimorphic differences in genome activity, which is orchestrated by transcription factors (TFs), could explain the differential response of male and female embryos to environmental stressors. To proof this hypothesis, the expression of cellular and extracellular TFs was investigated in male and female bovine embryos in vitro cultured either under low (5%) or high (20%) oxygen levels. The intracellular reactive oxygen species (ROS), total cell number, expression of nuclear factor (erythroid‐derived 2) factor 2 (NFE2L2), Krüppel‐like factor 4 (KLF4), notch receptor 1 (NOTCH1), E2F transcription factor 1 (E2F1), and SREBF2 along with extracellular vesicles (EVs) biogenesis genes were assessed at the blastocyst stage and their released EVs. Low blastocyst rate in both sexes due to oxidative stress (OS) was accompanied by increased ROS accumulation and reduced cell number in female embryos. The messenger RNA and protein levels of NFE2L2, as well as KLF4 expression, were higher in male embryos exposed to OS compared with female embryos. However, the expression of NOTCH1 and E2F1 was higher in female embryos cultured in high oxygen level. Male embryos exposed to OS released more EVs enriched with NFE2L2, superoxide dismutase 1, and NOTCH1 accompanied by elevated expression of EVs biogenesis genes. Accordingly, differential expression of TFs and their release into spent media could partially explain the sexual dimorphic response of bovine embryos to environmental stresses.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Sexual dimorphic expression and release of transcription factors in bovine embryos exposed to oxidative stress

Taqi

Saeed‐Zidane

Gebremedhn

et al. 2019

Molecular Reproduction Devel

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“…Many supplements have been tested to support embryo development. FCS is one of the most common, though its use is controversial, and it has been shown to increase hatching rates, provide more consistent results (Wydooghe et al, ), or protect male embryos against oxidative stress (Dallemagne et al, ). Moreover, low FCS concentrations (0.1%) combined with bovine serum albumin (BSA) have been found to increase blastocyst rates without altering embryo quality in terms of apoptosis, lipid accumulation, and pregnancy (Murillo et al, ).…”

Section: Embryo Responses To Art‐induced Stress In Livestockmentioning

confidence: 99%

Embryo responses to stress induced by assisted reproductive technologies

Ramos‐Ibeas

Heras

Gómez-Redondo

et al. 2019

Molecular Reproduction Devel

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Assisted reproductive technology (ART) has led to the birth of millions of babies. In cattle, thousands of embryos are produced annually. However, since the introduction and widespread use of ART, negative effects on embryos and offspring are starting to emerge. Knowledge so far, mostly provided by animal models, indicates that suboptimal conditions during ART can affect embryo viability and quality, and may induce embryonic stress responses. These stress responses take the form of severe gene expression alterations or modifications in critical epigenetic marks established during early developmental stages that can persist after birth. Unfortunately, while developmental plasticity allows the embryo to survive these stressful conditions, such insult may lead to adult health problems and to long-term effects on offspring that could be transmitted to subsequent generations. In this review, we describe how in mice, livestock, and humans, besides affecting the development of the embryo itself, ART stressors may also have significant repercussions on offspring health and physiology. Finally, we argue the case that better control of stressors during ART will help improve embryo quality and offspring health. K E Y W O R D S assisted reproductive technologies, bovine, embryo, human, mouse, stress

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“…Pro-oxidants have extensive sources and can be generated by the products of cellular respiration and steroid metabolism. The oxidative stress during embryo culture has been associated with lower developmental potential [ 23 ], changes in gene expression [ 24 ], histone remodeling [ 25 ], and increased apoptotic rates [ 26 ]. Oxidative stress is a cellular condition that may play a role in early embryo development, especially when oocytes are collected from females in different reproductive phases (estrous/anestrous/pregnancy), however, currently, there is a lack of direct supporting evidence.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor

Korzekwa

Kotlarczyk

Szczepańska

et al. 2020

Animals

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The aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups, including the ovaries collected post mortem on the 4th and 13th days of the estrous cycle and during pregnancy (n = 18). After oocyte maturation, frozen-thawed epididymal semen was used for IVF. Blastocyst quality, apoptotic potential by determining the mRNA expression of BAX, BCL-2, OCT4, SOX2, and placenta-specific 8 gene (PLAC8), and antioxidative potential of blastocysts were evaluated by determining the mRNA expression of CuSOD, MnSOD, and GPX as well as the enzymatic activity of superoxide dismutase and reduced glutathione. The highest development rate of expanded blastocyst, mRNA expression of BCL-2, OCT4, SOX2, and PLAC8 and mRNA expression and enzymatic activity of the antioxidative factors increased (p < 0.05) in blastocysts developed from the oocytes collected on the 4th day, compared to those developed from the oocytes collected on the 13th day of the cycle and during pregnancy. Our study indicates that the 4th day of the estrous cycle is the most effective period for oocyte collection for IVF and embryo development in hinds, considering quality parameters and antioxidative potential of the blastocysts.

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O xidative stress differentially impacts male and female bovine embryos depending on the culture medium and the stress condition

Cited by 12 publications

References 52 publications

Sexual dimorphic expression and release of transcription factors in bovine embryos exposed to oxidative stress

Sexual dimorphic expression and release of transcription factors in bovine embryos exposed to oxidative stress

Embryo responses to stress induced by assisted reproductive technologies

Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor

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