O sistema renina-angiotensina na doença periodontal induzida experimentalmente em ratos

Lima, Marta Lopes

doi:10.11606/t.25.2011.tde-18012012-105831

Cited by 5 publications

(13 citation statements)

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“…A doença periodontal experimentalmente induzida em ratos mostrou-se bem estabelecida nos grupos, ao passo que, foi capaz de promover a perda óssea alveolar no presente estudo, assim como em outros trabalhos (MACIEL, 2013;LIMA, 2011;OLIVEIRA, 2007). Na figura 15, podemos notar que ao compararmos os grupos tratados com veículo ao grupo tratado com captopril, é possível observar que o captopril não foi capaz de diminuir a perda óssea alveolar, independente do tempo de tratamento e da existência ou não de um pré-tratamento.…”

Section: Discussão Dos Resultadosunclassified

“…Outras drogas que interferem no SRA, alisquireno e losartan, demonstraram efetividade com relação à perda óssea alveolar durante a doença periodontal experimentalmente induzida em ratos (LIMA, 2011). Isto se deve ao fato da importante diminuição da interação da Ang II com os receptores AT1, uma vez que esta interação promove a ativação de osteoclastos (LI et al, 2008).…”

Section: Discussão Dos Resultadosunclassified

“…A metodologia do presente estudo foi baseada em trabalhos prévios, nos quais foi utilizada a mesma técnica de indução da doença periodontal experimental, os quais relatam que o período de maior ação de mediadores do processo inflamatório ocorre nas duas semanas após a colocação da ligadura (BEZERRA et al, 2000;RODINI et al, 2008;OLIVEIRA et al, 2008;DE ALMEIDA, 2008;GARCIA et al, 2009;LIMA et al, 2011). Por isso, a escolha dos tempos de tratamento de 14 e 21 após a indução da doença periodontal.…”

Section: Discussão Da Metodologiaunclassified

“…Com relação à indução da doença periodontal em ratos, a metodologia escolhida consiste na colocação de uma ligadura ao redor da região cervical do primeiro molar inferior (MADDEN;CATON, 1994;LIMA, 2011;MACIEL, 2013). Com a ligadura, ocorre um acúmulo progressivo de bactérias presentes no próprio ambiente bucal do animal.…”

Section: Discussão Da Metodologiaunclassified

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Efeitos do captopril sobre a doença periodontal induzida experimentalmente em ratos

Souza¹

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Effects of captopril on experimentally-induced periodontal disease induced in rats Periodontal disease (PD) consists of a group of inflammatory diseases which result in the destruction of tooth supporting structures. They are of infectious nature, with etiological factors related to gram-negative microorganisms, and may have manifestations in several ways. These comprise a variety of factors that allow the increase in its virulence and ability to multiply and persist in the periodontal tissue. Recent findings form our laboratory showed that mRNA expression exists in rat gingival tissue for all components of the Renin-Angiotensin System (RAS), the presence of renin as well as Angiotensin Converting Enzyme I (ACE) activity in rat gingival tissue, thus suggesting a possible correlation between the RAS and periodontal disease. Therefore, the aim of this study was to investigate whether captopril, an ACE inhibitor, alters the progression of experimentally-induced PD in rats. Thus, the model of PD induction by ligature placement around rat lower first molar was used. Animals were divided groups of 10 animals each, which were treated with captopril (via gavage, 30 mg/kg/day) or water (vehicle). Pre-treatment with this drug during 7 or 14 days was performed previously to PD induction, and after this period captopril was administered during 14 or 21 days. In addition, fictitious operation (SHAM group) was performed to induce PD. The techniques used in this study were: PD induction in rats, total RNA extraction, reverse transcriptionquantitative polymerase chain reaction (RT-qPCR) and alveolar bone loss. Data were analyzed by means of graphs. All the results were subjected to one-way analysis of variance (ANOVA) and represented means and respective standard errors. Differences between groups were considered statistically significant when p <0.05. Based on the results obtained in this study, it was concluded that captopril was not able to decrease bone loss in experimentally-induced PD in rats, although this drug altered the expression of mRNA for one RAS target (AT1a) and some mediators of inflammation in periodontal tissue such as, COX-2, ACE-2, IL-6, RANKL, VEGF-R1 and VEGF-R2.

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Section: Discussão Dos Resultadosunclassified

Section: Discussão Da Metodologiaunclassified

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Efeitos do captopril sobre a doença periodontal induzida experimentalmente em ratos

Souza¹

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“…O modelo de doença periodontal experimentalmente induzida em ratos utilizado em estudos anteriores (SOUZA, 2014;MACIEL, 2013;LIMA, 2011;OLIVEIRA et al, 2008, RODINI, 2005, mostrou-se efetivo e bem estabelecido nos grupos, capaz de promover a perda óssea alveolar.…”

Section: Osteoprotegerina (Opg)unclassified

Efeitos do ramipril sobre a doença periodontal induzida experimentalmente em ratos

Akashi¹

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Efeitos da inibição da enzima conversora de angiotensina sobre a doença periodontal induzida experimentalmente em ratos

Maciel¹

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Periodontal disease (PD) comprises a group of injuries affecting the periodontal tissue protection (gingivitis) and support (periodondite), involving the participation of resident cells, structural cells and inflammatory mediators. Recent research has shown the existence of a Renin Angiotensin System (RAS) site in the gingival tissue of rats and suggested that the SRA is involved in the onset of progression of PD experimentally induced in rats. Therefore, the aim of this study was to evaluate whether enalapril, angiotensin converting enzyme (ACE), bone loss and reduces the expression of RAS components in the gingival tissue. For this model was used for induction of PD placement of ligature around the mandibular first molar of rats and treatment of these animals with enalapril (20 mg / kg / day gavage) being used for micro-CT analysis of bone volume. The experimental groups were as follows (n = 5 each): Group 1-the pre-enalapril treatment for 14 days, and DP induction of post-treatment for 14 days, Group 2-the pre-enalapril treatment for 14 days, induction of DP and after treatment for 7 days and Group 3-pre-enalapril treatment for 7 days, induction of PD-and post-treatment for 14 days, and Group 4-the preenalapril treatment for 7 days, induction of post-treatment DP, and for 7 days. For comparison, all groups, and treatment with enalapril other animals received water (n = 5) and other (n = 5), sham surgery was performed to induce PD (sham). Analyses of alveolar bone loss and polymerase chain reaction quantitative (qPCR) of the following RAS components (angiotensinogen, ACE, ACE-2 receptor and AT1a, AT1b, AT2 and Mas). The data were properly analyzed by means of graphs, by using the t test for comparison of animals treated with enalapril or water with their sham, adopting a significance level of 5%. The results showed that only in the group with the shortest pre-treatment with enalapril (Group 3) showed no blocking of bone loss, but the other groups was statistically significant decrease in this parameter (Groups 1, 2 and 3). Regarding the molecular analysis of all targets tested, only the expression of angiotensinogen and Mas receptor was altered, with a statistically significant increase in the expression of mRNA for this enzyme identified in group 4. In conclusion, pre-treatment with enalapril ACE inhibitor can prevent bone loss in PD model experimentally induced in rats.

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O sistema renina-angiotensina na doença periodontal induzida experimentalmente em ratos

Cited by 5 publications

References 69 publications

Efeitos do captopril sobre a doença periodontal induzida experimentalmente em ratos

Efeitos do captopril sobre a doença periodontal induzida experimentalmente em ratos

Efeitos do ramipril sobre a doença periodontal induzida experimentalmente em ratos

Efeitos da inibição da enzima conversora de angiotensina sobre a doença periodontal induzida experimentalmente em ratos

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