O-serogroups of multi-drug resistant cervicovaginal Escherichia coli harboring a battery of virulence genes

Paniagua-Contreras, Gloria Luz; Monroy-Pérez, Eric; Solis, Rogelio Reyes; Cerón, Areli Bautista; Cortés, Luis Rey García; Nolasco-Alonso, Nancy; Camarillo, Daniela Hernández; Arreygue, Leopoldo Santillán; Domínguez-Trejo, Pablo; Velásquez, Clara Díaz; Vaca-Paniagua, Felipe; Uribe-García, Alina; Vaca, Sergio

doi:10.1016/j.jiac.2019.02.004

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…This result agrees with the high rates of resistance to ampicillin (94.6%), tetracycline (92.4%), nalidixic acid (88.6%), gentamicin (77.2%), amikacin (68.9%), and trimethoprim-sulfamethoxazole (67.4%) previously identified in CVEC strains [5]. The frequency of the genotype of resistance to beta-lactams, trimethoprim, sulfamethoxazole, aminoglycosides and quinolone coincides with the resistance phenotype, evaluated by the Kirby-Bauer method, recently published by our working group for these same CVEC strains [29]. The frequencies of the genes aac(3)II, tetA, and dfrA1 in the evaluated E. coli strains were higher than those in uropathogenic strains of E. coli in Iran, whereas the frequencies of the genes qnrA and sul1 were similar to those in these strains [15].…”

Section: Discussionmentioning

confidence: 99%

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

et al. 2020

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The pathogenicity of Escherichia coli strains that cause cervico-vaginal infections (CVI) is due to the presence of several virulence genes. The objective of this study was to define the variability regarding the genotype of antibiotic resistance, the transcription profiles of virulence genes after in vitro infection of the vaginal cell line A431 and the phylogroup composition of a group of cervico-vaginal E. coli strains (CVEC). A total of 200 E. coli strains isolated from Mexican women with CVI from two medical units of the Mexican Institute of Social Security were analysed. E. coli strains and antibiotic resistance genes were identified using conventional polymerase chain reaction (PCR), and phylogroups were identified using multiplex PCR. Virulence gene transcription was measured through reverse-transcriptase realtime PCR after infection of the vaginal cell line A431. The most common antibiotic resistance genes among the CVEC strains were aac(3)II, TEM, dfrA1, sul1, and qnrA. The predominant phylogroup was B2. The genes most frequently transcribed in these strains were fimH, papC, irp2, iroN, kpsMTII, cnf1, and ompT, mainly in CVEC strains isolated from chronic and occasional vaginal infections. The strains showed a large diversity of transcription of the virulence genes phenotype and antibiotic resistance genotype, especially in the strains of phylogroups, B2, A, and D. The strains formed 2 large clusters, which contained several subclusters. The genetic diversity of CVEC strains was high. These strains have a large number of transcription patterns of virulence genes, and one-third of them carry three to seven antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 99%

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

et al. 2020

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“…In addition, ESBL-producing organisms exhibit co-resistance to many other classes of antibiotics resulting in limitation of therapeutic options. Vaginal E. coli represents a real threat especially to neonates; however, little information is available regarding its antibiotic resistance [89,90]. The resistance rate of Iraqi local cervicovaginal E. coli to different antibiotic classes is summarized in Table 6.…”

Section: Expec-associated Vaginosis and Antibiotic Resistancementioning

confidence: 99%

Antibiotic Resistance among Iraqi LocalE. coliIsolates

Al-Dahmoshi¹,

Al-Khafaji²,

Al-Allak³

2020

E. Coli Infections - Importance of Early Diagnosis and Efficient Treatment

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Escherichia coli is a famous Gram-negative bacillary bacterium that belongs to Enterobacteriaceae. It is either micro-biota innocent for human or pathogenic with arrays of diseases. The pathogenic E. coli can be assigned to intestinal (InPEC) or extraintestinal (ExPEC) with disease ranging from watery diarrhea to pulmonary distress. The most prevalent form of InPEC is enteropathogenic E. coli (EPEC), while the most prevalent ExPEC is uropathogenic E. coli (UPEC). The other InPEC includes Shiga toxin-producing (STEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), enteroinvasive (EIEC), diffusely adherent (DAEC) and adherent invasive E. coli (AIEC). ExPEC was implicated in cystitis, pyelonephritis, sepsis, respiratory tract infection, cervicovaginal infection (CVEC), meningitis (NMEC), otitis media, cholecystitis and wound infection. Antibiotic resistance is the challenging in world nowadays. Multidrug-resistant (MDR) Escherichia coli has become challenging with existing antibiotic options. E. coli pathogens have various virulence factors that determine their pathogenesis and antimicrobial resistance mechanisms. The rapid and ongoing spread of antimicrobial-resistant organisms threatens our ability to successfully treat a growing number of infectious diseases. It is well established that antibiotic use is a significant, and modifiable, driver of antibiotic resistance. The most commonly used antibiotic classes for InPEC and ExPEC were third-generation cephalosporin, carbapenem, fluoroquinolone and aminoglycosides. Actually, the most effective prescribed medication is one of the following: cefotaxime, ceftriaxone, ciprofloxacin, amikacin, gentamycin, levofloxacin and imipenem. Generally, according to our review for more than 100 local Iraqi studies among Iraqi provinces, the results revealed the resistance rate from highest to lowest as follows: cefotaxime (76.5%), ceftriaxone (75.9%), gentamycin (41.65%), ciprofloxacin (32.13%), amikacin (17.3%), levofloxacin (15%) and imipenem (5.14%). The resistance mechanisms may include genes encoding antibiotic-modifying enzymes like those of extended-spectrum beta-lactamases gene comprising: blaCTX-M, blaTEM, blaSHV, blaOXA, blaPER, blaVIM, blaIMP, blaNDM and blaAMPc. Efflux pumping includes AcrAB, while resistance to quinolone may be mediated by mutation among qnrA, qnrB, qnrD and qnrS. Resistance to aminoglycosides includes encoding to aminoglycosidemodifying enzymes like aac(6)-Ib, aph(3)-I, aph(3)-IIa, aph(3)-Ib, ant(3)-I, aac(3)-II and aac(3)-IV.

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“…High resistance to beta-lactam antibiotics, primarily AM, has also been reported in strains of E. coli isolated from patients with periodontitis and gingivitis [ 38 ]. In a recent study, we discovered comparable resistance rates to AM, CB, CF, NET, and GE in cervicovaginal strains of E. coli [ 39 ]. The high resistance rate of strains to beta-lactam antibiotics found in this study may be due to the substantial increase in E. coli -producing extended-spectrum beta-lactamase (ESBL), both within the community and in hospitals [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of these virulence markers in the strains, in addition to the presence of the malX marker of the PAIs ( Table 2 ) may increase the risk of acquiring other types of infections. This is evident by the fact that we identified these genes in strains of E. coli responsible for cervicovaginal infections [ 39 ], whereas other authors have identified them in UPEC strains causing urinary tract infections [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

High Virulence and Multidrug Resistance of Escherichia coli Isolated in Periodontal Disease

et al. 2022

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Periodontal disease is caused by different gram-negative anaerobic bacteria; however, Escherichia coli has also been isolated from periodontitis and its role in periodontitis is less known. This study aimed to determine the variability in virulence genotype, antibiotic resistance phenotype, biofilm formation, phylogroups, and serotypes in different emerging periodontal strains of Escherichia coli, isolated from patients with periodontal disease and healthy controls. E. coli, virulence genes, and phylogroups, were identified by PCR, antibiotic susceptibility by the Kirby-Bauer method, biofilm formation was quantified using polystyrene microtiter plates, and serotypes were determined by serotyping. Although E. coli was not detected in the controls (n = 70), it was isolated in 14.7% (100/678) of the patients. Most of the strains (n = 81/100) were multidrug-resistance. The most frequent adhesion genes among the strains were fimH and iha, toxin genes were usp and hlyA, iron-acquisition genes were fyuA and irp2, and protectin genes were ompT, and KpsMT. Phylogroup B2 and serotype O25:H4 were the most predominant among the strains. These findings suggest that E. coli may be involved in periodontal disease due to its high virulence, multidrug-resistance, and a wide distribution of phylogroups and serotypes.

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O-serogroups of multi-drug resistant cervicovaginal Escherichia coli harboring a battery of virulence genes

Cited by 5 publications

References 26 publications

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

Antibiotic Resistance among Iraqi LocalE. coliIsolates

High Virulence and Multidrug Resistance of Escherichia coli Isolated in Periodontal Disease

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