O passado esquecido e o presente trágico

Barreiro, José Carlos; Ferreira, Ricardo Alexandre

doi:10.1590/1980-436920170000000036

Cited by 2 publications

(2 citation statements)

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“…As CD9 and CD29 also act as components of the same protein complex-proven by immunoprecipitation-reduction of both proteins in the plasma membrane might synergistically result in an impaired adhesion of the cells, associated with a lower cell index. Considering the effect on CD9, it is of particular interest that this tetraspanin plays a role in the interaction of EC with leucocytes in inflammatory processes, and one could therefore speculate that its depletion from the plasma membrane of retinal endothelial cells induced by sitagliptin might be at least in part also beneficial [39,40]. Whether or not prevention of inflammation in the retina of type 1 diabetic rats by sitagliptin is due to this process remains unresolved, although it definitely is an exciting speculation [11].…”

Section: Discussionmentioning

confidence: 99%

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Jäckle

Ziemssen

Kuhn

et al. 2020

Journal of Diabetes Research

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Inhibitors of dipeptidyl peptidase-4 (DPP-4) are widely used to treat diabetes mellitus, but data concerning their effects on the barrier stability of retinal endothelial cells (REC) in vivo and in vitro are inconsistent. Therefore, we studied whether the barrier properties of immortalized endothelial cells of the bovine retina (iBREC) were affected by the inhibitors of DPP-4 sitagliptin (10-1000 nM) and diprotin A (1-25 μM). Their effects were also investigated in the presence of VEGF-A165 because diabetic patients often develop macular edema caused by VEGF-A-induced permeability of REC. To detect even transient or subtle changes of paracellular and transcellular flow as well as adhesion of the cells to the extracellular matrix, we continuously monitored the cell index (CI) of confluent iBREC grown on gold electrodes. Initially, the CI remained stable but started to decline significantly and persistently at 40 h or 55 h after addition of sitagliptin or diprotin A, respectively. Both inhibitors did not modulate, prevent, or revert the persistent VEGF-A165-induced reduction of the CI. Interestingly, sitagliptin and diprotin A increased the expression of the tight-junction protein claudin-1 which is an important component of a functional barrier formed by iBREC. In contrast, expressions of CD29—a subunit of the fibronectin receptor—or of the tetraspanin CD9 were lower after extended treatment with the DPP-4 inhibitors; less of the CD9 was seen at the plasma membrane after prolonged exposure to sitagliptin. Because both associated proteins are important for adhesion of iBREC to the extracellular matrix, the observed low CI might be caused by weakened attachment of the cells. From our results, we conclude that extended inhibition of DPP-4 destabilizes the barrier formed by microvascular REC and that DPP-4 inhibitors like sitagliptin do not counteract or enhance a VEGF-A165-induced barrier dysfunction as frequently observed in DME.

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Section: Discussionmentioning

confidence: 99%

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Jäckle

Ziemssen

Kuhn

et al. 2020

Journal of Diabetes Research

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“…The results of our study show that for surgical personnel negligence is the error with the greatest penal repercussion. However, other studies such as those presented by (Ruiz, 2011;Marcela, Helena, & Eduardo, 2011;Bernate-Ochoa, 2010;López Ordoñez et al, 2004;Lascariz Jiménez, 2000;Kunsemuller, 1986;Guzmán, Franco, & Morales, 1996) mention that the most frequent medical lawsuits according to the legal system information are caused by human factors that lead to malpractice, carelessness or recklessness with a criminal liability of a malicious or negligent nature (Marcela et al, 2011;Barreiro Ramos, Barreiro Peñaranda, Quesada Soto, Fernández Viera, & Marrero Martín, 2005;Guzmán et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Legal Implications for Actions Considered Iatrogenic: Knowledge and Practices of the Surgical Team, Valledupar, Cesar/Colombia

Anderson¹,

Peralta²,

Montero³

et al. 2020

GJHS

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Introduction:The errors considered iatrogenic are caused by different causes that are mostly considered foreseeable, including inaccuracy, recklessness and ignorance, all these actions with ethical-legal repercussions. In Colombia, despite not having studies that position iatrogeny as one of the main causes of death. It is estimated that in Colombia 180,000 people die each year for this reason. Iatrogeny is described in the USA as the third leading cause of death. Objective: To evaluate the knowledge and aspects related to negligence, inexperience, recklessness, that the surgical team has in regard to its ethical-legal responsibilities. Methodology:Descriptive cross-sectional study. A total of 93 professionals were surveyed who are part of the surgical team of two health-care institutions in the department of Cesar, Colombia during 2019. Results:Surveyed professionals showed a 100% ignorance about the process to follow during an adverse event. It was identified that 32 % of the professionals and technicians surveyed do not have insurance to back them up legally. In addition, 31% said that errors such as negligence, recklessness and inexperience can occur in parallel with criminal and civil repercussions. The surgical team considers that the most frequent error are elements and instruments left in the cavity (oblites) in 64%. Conclusions:For surgical personnel, negligence is the error with the greatest penal repercussion if it is related to the death of the patient, surgery on the wrong patient and wrong side of the body, in addition to oblites or foreign bodies left in the cavity.

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O passado esquecido e o presente trágico

Abstract: O presente número da revista História (São Paulo) está constituído por dois dossiês, vários artigos livres e resenhas de importância inequívoca para o enriquecimento da discussão historiográfica do Brasil e também no âmbito internacional.

Cited by 2 publications

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Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure

Legal Implications for Actions Considered Iatrogenic: Knowledge and Practices of the Surgical Team, Valledupar, Cesar/Colombia

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