O'nyong-nyong fever: An epidemic virus disease in East Africa VIII. Virus isolations from anopheles mosquitoes

Williams, M. C.; Woodall, John; Corbet, Philip S.; Gillett, J. D.

doi:10.1016/0035-9203(65)90012-x

Cited by 98 publications

(64 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3][4] These viruses are an emerging threat because of their ability to initiate explosive epidemics, 3,5 including a 1979 -1980 epidemic of Ross River fever in the South Pacific, which involved more than 60,000 patients, 3 and a 1959 -1962 epidemic of O'nyong-nyong fever in Africa, which involved at least 2 million patients. 6 In 2004, reemergence of CHIKV resulted in an epidemic that caused millions of cases in multiple countries in the Indian Ocean region, including 270,000 cases on Reunion Island and an estimated 1.4 million to 6.5 million cases in India. 7,8 The ability of these viruses to spread in a human to mosquito to human transmission cycle in the absence of animal reservoirs is particularly concerning because this increases the likelihood of establishing epidemics in new areas, including Europe and the Western Hemisphere.…”

mentioning

confidence: 99%

A Mouse Model of Chikungunya Virus–Induced Musculoskeletal Inflammatory Disease

Morrison

Oko

Montgomery

et al. 2011

The American Journal of Pathology

241

224

View full text Add to dashboard Cite

Chikungunya virus (CHIKV), an emerging mosquitoborne Alphavirus, causes debilitating rheumatic disease in humans that can last for weeks to months. Starting in 2004, a CHIKV outbreak in the Indian Ocean region affected millions of people, and infected travelers introduced CHIKV to new regions. The pathogenesis of CHIKV is poorly understood, and no approved vaccines or specific therapies exist. A major challenge to the study of CHIKV disease is the lack of a small animal model that recapitulates the major outcomes of human infection. In this study, the pathogenesis of CHIKV in C57BL/6J mice was investigated using biological and molecular clones of CHIKV isolated from human serum (CHIKV SL15649). After 14-day-old mice were inoculated with CHIKV SL15649 in the footpad, they displayed reduced weight gain and swelling of the inoculated limb. Histologic analysis of hind limb sections revealed severe necrotizing myositis, mixed inflammatory cell arthritis, chronic active tenosynovitis, and multifocal vasculitis. Interestingly, these disease signs and viral RNA persisted in musculoskeletal tissues for at least 3 weeks after inoculation. This work demonstrates the development of a mouse model of CHIKV infection with clinical manifestations and histopathologic findings that are consistent with the disease signs of CHIKV-infected humans, providing a useful tool for studying viral and host factors that drive CHIKV pathogenesis and for evaluating potential therapeutics against this emerging viral disease.

show abstract

mentioning

confidence: 99%

A Mouse Model of Chikungunya Virus–Induced Musculoskeletal Inflammatory Disease

Morrison

Oko

Montgomery

et al. 2011

The American Journal of Pathology

241

224

View full text Add to dashboard Cite

show abstract

“…In D. melanogaster a number of miRNAs have been implicated in regulating immune responses (reviewed by [5,122] ), such as miR-8 [21] and the let-7 miRNA [35] which are involved in regulation of antimicrobial peptides. Recently, miRNAs involved in controlling immune responses such as melanisation have also been predicted by a computational approach in the mosquito species A. gambiae [118], the vector of O'nyong-nyong virus (ONNV) [127]. In A. aegypti mosquitoes, miRNAs are responsible for gene expression changes after a bloodmeal, and one of these (aae-miR-375) regulates immunerelated genes such as cactus and REL1 [54].…”

Section: Mirnasmentioning

confidence: 99%

Antiviral responses of arthropod vectors: an update on recent advances

et al. 2014

View full text Add to dashboard Cite

Arthropod vectors, such as mosquitoes, ticks, biting midges and sand flies, transmit many viruses that can cause outbreaks of disease in humans and animals around the world. Arthropod vector species are invading new areas due to globalisation and environmental changes, and contact between exotic animal species, humans and arthropod vectors is increasing, bringing with it the regular emergence of new arboviruses. For future strategies to control arbovirus transmission, it is important to improve our understanding of virus-vector interactions. In the last decade knowledge of arthropod antiviral immunity has increased rapidly. RNAi has been proposed as the most important antiviral response in mosquitoes and it is likely to be the most important antiviral response in all arthropods. However, other newly-discovered antiviral strategies such as melanisation and the link between RNAi and the JAK/STAT pathway via the cytokine Vago have been characterised in the last few years. This review aims to summarise the most important and most recent advances made in arthropod antiviral immunity.

show abstract

“…However, several large-scale epidemics have occurred, including one during 1959-1962 in northern Uganda that involved more than two million cases. 10,11 In 1996, ONNV reappeared after a 35-year hiatus and was responsible for causing another epidemic in Uganda. 12,13 The most recent outbreak of ONNV was documented in the fall of 2003 and involved refugees from Western Côte d'Ivoire, an area not normally associated with ONNV transmission.…”

Section: Introductionmentioning

confidence: 99%

“…funestus and, as observed for Asian CHIKV, it may be maintained in nature by human-to-human transmission by its mosquito vector. 10,11,15 Currently, the diagnosis of CHIK and ONN is determined by either virus isolation, detection of virus-specific antibodies by enzyme-linked immunosorbent assay, or by genomic detection using reverse transcription-polymerase chain reaction (RT-PCR). Several groups have developed real-time RT-PCR assays for the molecular diagnosis of CHIK involving human samples.…”

Section: Introductionmentioning

confidence: 99%

Development of Field-Based Real-Time Reverse Transcription–Polymerase Chain Reaction Assays for Detection of Chikungunya and O’nyong-nyong Viruses in Mosquitoes

Smith

Lee

Jahrling

et al. 2009

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Chikungunya (CHIK) and O'nyong-nyong (ONN) are important emerging arthropod-borne diseases. Molecular diagnosis of these two viruses in mosquitoes has not been evaluated, and the effects of extraneous mosquito tissue on assay performance have not been tested. Additionally, no real-time reverse transcription-polymerase chain reaction (RT-PCR) assay exists for detecting ONN virus (ONNV) RNA. We describe the development of sensitive and specific real-time RT-PCR assays for detecting CHIK and ONN viral RNA in mosquitoes, which have application for field use. In addition, we compared three methods for primer/probe design for assay development by evaluating their sensitivity and specificity. This comparison resulted in development of virus-specific assays that could detect less than one plaqueforming unit equivalent of each of the viruses in mosquitoes. The use of these assays will aid in arthropod-borne disease surveillance and in the control of the associated diseases.

show abstract

O'nyong-nyong fever: An epidemic virus disease in East Africa VIII. Virus isolations from anopheles mosquitoes

Cited by 98 publications

References 5 publications

A Mouse Model of Chikungunya Virus–Induced Musculoskeletal Inflammatory Disease

A Mouse Model of Chikungunya Virus–Induced Musculoskeletal Inflammatory Disease

Antiviral responses of arthropod vectors: an update on recent advances

Development of Field-Based Real-Time Reverse Transcription–Polymerase Chain Reaction Assays for Detection of Chikungunya and O’nyong-nyong Viruses in Mosquitoes

Contact Info

Product

Resources

About