O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity

Florman, Harvey M.; Wassarman, Paul M.

doi:10.1016/0092-8674(85)90084-4

Cited by 564 publications

(347 citation statements)

References 79 publications

Supporting

Mentioning

330

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, in a diverse range of organisms (from echinoderms to mammals), the recognition of carbohydrate epitopes by complementary protein receptors has been proposed to be a critical factor in gamete interaction [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…In mammals such as mice and pigs, it is generally accepted that carbohydrate epitopes displayed by zona pellucida glycoprotein 3 (ZP3) play a pivotal role in spermatozoa-ZP3 interaction [2,7]. However, although both native human zona pellucida and purified, glycosylated recombinant human ZP3 (rhuZP3) secreted by Chinese Hamster Ovary (CHO) cells have been shown to stimulate acrosomal exocytosis in human spermatozoa [8][9][10][11], it has yet to be firmly established whether the ability Abbreviations used : IPTG, isopropyl-β-D-thiogalactopyranoside ; OD 595 , optical density at 595 nm ; rhuZP3, recombinant human ZP3 ; GST-HuZP3, glutathione S-transferase-recombinant human ZP3 fusion protein ; TTB, Towbin Transfer Buffer.…”

Section: Introductionmentioning

confidence: 99%

“…1 Present address : Department of Biochemistry, Wellcome Trust Building, University of Dundee, Dundee, DD1 4HN, Scotland. 2 To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

“…One method to examine the role of the protein backbone in gamete interaction has been to deglycosylate zona proteins either enzymatically or chemically [2,7]. Although both approaches successfully remove carbohydrate from the polypeptide, there are a number of limitations in such experiments.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

Chapman

Kessopoulou

Andrews

et al. 1998

Biochemical Journal

View full text Add to dashboard Cite

Human gamete interaction is of fundamental biological importance, yet the molecular interactions between spermatozoa and the zona pellucida are poorly understood. Surprisingly, the role of the polypeptide backbone of zona pellucida glycoprotein 3 (ZP3), the putative ligand for spermatozoa activation, has been largely overlooked. Purified recombinant human ZP3 was expressed in Escherichia coli as a C-terminal fusion to the dimeric glutathione S-transferase (GST) from Schistosoma japonicum and was shown to induce acrosomal exocytosis in live, capacitated human spermatozoa. The level of exocytosis is comparable with that obtained using purified, glycosylated, recombinant human ZP3 [van Duin, M., Polman, J. E. M., DeBreet, I. T. M., Van Ginneken, K., Bunschoten, H., Grootenhuis, A., Brindle, J. and Aitken, R. J. (1994). Biol Reprod. 51, 607-617]. These data imply that the polypeptide chain of human ZP3 contributes to recognition of spermatozoa during acrosomal exocytosis in vitro.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…1 Present address : Department of Biochemistry, Wellcome Trust Building, University of Dundee, Dundee, DD1 4HN, Scotland. 2 To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

Chapman

Kessopoulou

Andrews

et al. 1998

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…However, selective removal of N-linked oligosaccharides from mouse ZP3 by endo-b-N-acetyl-D-glucosamine treatment has no effect on the induction of the AR, whereas removal of O-linked oligosaccharides by alkaline hydrolysis abrogates its ability to induce the AR. 90 Initial studies implicated galactose in a-or b-linkages at the non-reducing terminus of O-linked oligosaccharides and N-acetylglucosamine (GlcNAc) in b-linkages as the sugar determinants on mouse ZP3 that are responsible for the binding of sperm to the ZP. 91 However, mice deficient in glycosyl transferase, which amends terminal galactose in an a-linkage, are fully fertile 92,93 implicating galactose in b-linkages or GlcNAc or both as critical residues.…”

Section: Zp4mentioning

confidence: 99%

Acrosome reaction: relevance of zona pellucida glycoproteins

Gupta

Bhandari²

2010

Asian J Androl

View full text Add to dashboard Cite

During mammalian fertilisation, the zona pellucida (ZP) matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction (AR) in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four (ZP1, ZP2, ZP3 and ZP4). ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans (in addition to ZP3), ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the G i -coupled receptor pathway, whereas ZP1-and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels (VOCCs), whereas ZP1-and ZP4-induced ARs involve both T-and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans (in addition to ZP3), ZP1 and ZP4 also participate in these stages of fertilisation. Keywords: acrosome reaction; fertilisation; oocyte; signalling pathways; spermatozoa; zona pellucida glycoproteins INTRODUCTION Mammalian fertilisation is a highly synchronized process that involves a complex series of interactions between the spermatozoon and the egg, culminating in their unison. The initial steps in fertilisation involves the binding of the spermatozoon to the zona pellucida (ZP) matrix surrounding the egg, followed by induction of the acrosome reaction (AR) in the zona-bound spermatozoon, a pre-requisite for penetration of the ZP matrix by the spermatozoon. The spermatozoon acrosome is a Golgi-derived organelle that forms a cap over the anterior two-thirds of its nucleus. The AR involves the fusion of the sperm membrane with the outer acrosomal membrane, resulting in release of the acrosomal contents and exposure of the inner acrosomal membrane on the anterior head of the spermatozoon. Various physiological agents, such as progesterone, serum albumin, follicular fluid, hormones (including biogenic amines), hydrolytic enzymes (particularly proteases), hyaluronic acid and ZP glycoproteins, have been implicated in the induction of the AR. [1][2][3] In the present paper, we review the role of the ZP matrix and its constituents in AR induction. Various downstream signalling pathways involved in the ZP glycoprotein-induced AR will also be discussed. On the basis of the current literature and studies from our group, the salient differences in the ZP glycoprotein-mediated induction of the AR in mouse versus human will be highlighted.

show abstract

Structure and function of the mammalian egg zona pellucida

et al. 1999

View full text Add to dashboard Cite

The zona pellucida is a thick extracellular coat that surrounds all mammalian eggs and preimplantation embryos. The zona pellucida supports communication between oocytes and follicle cells during oogenesis; protects oocytes, eggs, and embryos during development, and regulates interactions between ovulated eggs and free‐swimming sperm during and following fertilization. Mutant females that produce eggs that lack a zona pellucida are infertile. The functions of the zona pellucida during fertilization now can be ascribed to certain of its glycoproteins. Here we describe some aspects of zona pellucida structure and function as they relate to mammalian fertilization. J. Exp. Zool. (Mol. Dev. Evol.) 285:251–258, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity

Cited by 564 publications

References 79 publications

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro

Acrosome reaction: relevance of zona pellucida glycoproteins

Structure and function of the mammalian egg zona pellucida

Contact Info

Product

Resources

About